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Effect of long-term prazosin and yohimbine administration on c-Fos expression in spinal neurons: inhibitory effect of alpha-1 and alpha-2 adrenoceptors on afferents from the lower urinary tract.
Urol Int. 2011; 87(2):230-7.UI

Abstract

INTRODUCTION

To investigate the effects of long-term administration of the α(1)-adrenoceptor antagonist prazosin and the α(2)-adrenoceptor antagonist yohimbine on afferent inputs from the lower urinary tract by evaluating c-Fos expression in the spinal cord.

MATERIALS AND METHODS

Prazosin or yohimbine was administered for 4 weeks in rats using an osmotic pump. Effects of these agents on urodynamic parameters were determined by continuous cystometry in conscious rats. After cystometry, c-Fos expression in the dorsal horn of the L6 spinal cord was measured by immunohistochemistry.

RESULTS

The administration of prazosin (0.12 mg/kg/day) or yohimbine (0.10 mg/kg/day) significantly increased micturition interval and bladder capacity, but did not affect micturition pressure and residual urine volume. The numbers of c-Fos-positive neurons in the dorsal horn were significantly lower in rats that received prazosin than in controls. Yohimbine reduced the number of c-Fos-positive neurons in part of the dorsal horn.

CONCLUSIONS

Long-term administration of prazosin and yohimbine at clinically recommended doses can exert inhibitory effects on afferent pathways from the lower urinary tract during the storage phase. These reductions of the afferent input result in the increased bladder capacity and increased micturition interval.

Authors+Show Affiliations

Department of Urology, Fukushima Medical University School of Medicine, Fukushima, Japan. pessoco@fmu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21525724

Citation

Haga, Nobuhiro, et al. "Effect of Long-term Prazosin and Yohimbine Administration On c-Fos Expression in Spinal Neurons: Inhibitory Effect of Alpha-1 and Alpha-2 Adrenoceptors On Afferents From the Lower Urinary Tract." Urologia Internationalis, vol. 87, no. 2, 2011, pp. 230-7.
Haga N, Aikawa K, Shishido K, et al. Effect of long-term prazosin and yohimbine administration on c-Fos expression in spinal neurons: inhibitory effect of alpha-1 and alpha-2 adrenoceptors on afferents from the lower urinary tract. Urol Int. 2011;87(2):230-7.
Haga, N., Aikawa, K., Shishido, K., Yanagida, T., Kushida, N., Takahashi, N., Yazaki, J., & Yamaguchi, O. (2011). Effect of long-term prazosin and yohimbine administration on c-Fos expression in spinal neurons: inhibitory effect of alpha-1 and alpha-2 adrenoceptors on afferents from the lower urinary tract. Urologia Internationalis, 87(2), 230-7. https://doi.org/10.1159/000324274
Haga N, et al. Effect of Long-term Prazosin and Yohimbine Administration On c-Fos Expression in Spinal Neurons: Inhibitory Effect of Alpha-1 and Alpha-2 Adrenoceptors On Afferents From the Lower Urinary Tract. Urol Int. 2011;87(2):230-7. PubMed PMID: 21525724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of long-term prazosin and yohimbine administration on c-Fos expression in spinal neurons: inhibitory effect of alpha-1 and alpha-2 adrenoceptors on afferents from the lower urinary tract. AU - Haga,Nobuhiro, AU - Aikawa,Ken, AU - Shishido,Keiichi, AU - Yanagida,Tomohiko, AU - Kushida,Nobuhiro, AU - Takahashi,Norio, AU - Yazaki,Junji, AU - Yamaguchi,Osamu, Y1 - 2011/04/27/ PY - 2010/10/11/received PY - 2011/01/10/accepted PY - 2011/4/29/entrez PY - 2011/4/29/pubmed PY - 2012/1/18/medline SP - 230 EP - 7 JF - Urologia internationalis JO - Urol. Int. VL - 87 IS - 2 N2 - INTRODUCTION: To investigate the effects of long-term administration of the α(1)-adrenoceptor antagonist prazosin and the α(2)-adrenoceptor antagonist yohimbine on afferent inputs from the lower urinary tract by evaluating c-Fos expression in the spinal cord. MATERIALS AND METHODS: Prazosin or yohimbine was administered for 4 weeks in rats using an osmotic pump. Effects of these agents on urodynamic parameters were determined by continuous cystometry in conscious rats. After cystometry, c-Fos expression in the dorsal horn of the L6 spinal cord was measured by immunohistochemistry. RESULTS: The administration of prazosin (0.12 mg/kg/day) or yohimbine (0.10 mg/kg/day) significantly increased micturition interval and bladder capacity, but did not affect micturition pressure and residual urine volume. The numbers of c-Fos-positive neurons in the dorsal horn were significantly lower in rats that received prazosin than in controls. Yohimbine reduced the number of c-Fos-positive neurons in part of the dorsal horn. CONCLUSIONS: Long-term administration of prazosin and yohimbine at clinically recommended doses can exert inhibitory effects on afferent pathways from the lower urinary tract during the storage phase. These reductions of the afferent input result in the increased bladder capacity and increased micturition interval. SN - 1423-0399 UR - https://www.unboundmedicine.com/medline/citation/21525724/Effect_of_long_term_prazosin_and_yohimbine_administration_on_c_Fos_expression_in_spinal_neurons:_inhibitory_effect_of_alpha_1_and_alpha_2_adrenoceptors_on_afferents_from_the_lower_urinary_tract_ L2 - https://www.karger.com?DOI=10.1159/000324274 DB - PRIME DP - Unbound Medicine ER -