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Antiprotozoal activities of traditional medicinal plants from the Garhwal region of North West Himalaya, India.
J Ethnopharmacol 2011; 136(1):123-8JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

In a search for new plant-derived biologically active compounds against protozoan parasites, an ethnopharmacological study was carried out to evaluate extracts from selected 17 traditional medicinal plants which were used by healers from the Garhwal region of North West Himalaya for the treatment of protozoal infections and fever including malaria.

MATERIALS AND METHODS

In vitro activity against erythrocytic stages of Plasmodium falciparum was determined using a modified [3H]-hypoxanthine incorporation assay with the chloroquine- and pyrimethamine-resistant K1 strain. Activity against Trypanosoma brucei rhodesiense was performed on the STIB 900 strain and activity against Trypanosoma cruzi on infected rat skeletal myoblasts (L6 cells) seeded in 96-well microtitre plates while amastigotes of Leishmania donovani strain MHOM/ET/67/L82 were used to assess activity against Leishmania donovani. Cytotoxicity assays were performed against rat skeletal myoblasts (L6-cells).

RESULTS AND CONCLUSIONS

Extracts of Artemisia roxburghiana, Roylea cinerea, Leucas cephalotes, Nepeta hindostana and Viola canescens showed good antiplasmodial activity (IC50<5 μg/ml). The chloroform extract of Artemisia roxburghiana was the most active (IC50 value of 0.42 μg/ml) and the most selective (SI=78) extract for Plasmodium falciparum among all plants extracts examined. The chloroform extract of Leucas cephalotes and the petroleum ether extract of Viola canescens exhibited substantial activities against Leishmania donovani with IC50 values of 3.61 μg/ml (SI=8) and 0.40 μg/ml (SI=30), respectively. The petroleum ether extract of Viola canescens exhibited activity against Trypanosoma cruzi with an IC50 value of 1.86 μg/ml (SI=7). Methanol and water extracts from all plants under investigation were found inactive against all parasites tested. These results support investigation of components of traditional medicines as potential new antiprotozoal agents. On the other hand since herbalism has become the main stream throughout the world, investigation demonstrates that these non-polar plant extracts of six of the plants examined in this study could play an important role in herbal formulations for the treatment of vector borne protozoal diseases.

Authors+Show Affiliations

National Institute of Malaria Research, Field Unit, Hardwar 249403, India. vkdua51@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21527328

Citation

Dua, Virendra K., et al. "Antiprotozoal Activities of Traditional Medicinal Plants From the Garhwal Region of North West Himalaya, India." Journal of Ethnopharmacology, vol. 136, no. 1, 2011, pp. 123-8.
Dua VK, Verma G, Agarwal DD, et al. Antiprotozoal activities of traditional medicinal plants from the Garhwal region of North West Himalaya, India. J Ethnopharmacol. 2011;136(1):123-8.
Dua, V. K., Verma, G., Agarwal, D. D., Kaiser, M., & Brun, R. (2011). Antiprotozoal activities of traditional medicinal plants from the Garhwal region of North West Himalaya, India. Journal of Ethnopharmacology, 136(1), pp. 123-8. doi:10.1016/j.jep.2011.04.024.
Dua VK, et al. Antiprotozoal Activities of Traditional Medicinal Plants From the Garhwal Region of North West Himalaya, India. J Ethnopharmacol. 2011 Jun 14;136(1):123-8. PubMed PMID: 21527328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiprotozoal activities of traditional medicinal plants from the Garhwal region of North West Himalaya, India. AU - Dua,Virendra K, AU - Verma,Gaurav, AU - Agarwal,Dau Dayal, AU - Kaiser,Marcel, AU - Brun,Reto, Y1 - 2011/04/16/ PY - 2010/12/20/received PY - 2011/03/29/revised PY - 2011/04/11/accepted PY - 2011/4/30/entrez PY - 2011/4/30/pubmed PY - 2011/12/31/medline SP - 123 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 136 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: In a search for new plant-derived biologically active compounds against protozoan parasites, an ethnopharmacological study was carried out to evaluate extracts from selected 17 traditional medicinal plants which were used by healers from the Garhwal region of North West Himalaya for the treatment of protozoal infections and fever including malaria. MATERIALS AND METHODS: In vitro activity against erythrocytic stages of Plasmodium falciparum was determined using a modified [3H]-hypoxanthine incorporation assay with the chloroquine- and pyrimethamine-resistant K1 strain. Activity against Trypanosoma brucei rhodesiense was performed on the STIB 900 strain and activity against Trypanosoma cruzi on infected rat skeletal myoblasts (L6 cells) seeded in 96-well microtitre plates while amastigotes of Leishmania donovani strain MHOM/ET/67/L82 were used to assess activity against Leishmania donovani. Cytotoxicity assays were performed against rat skeletal myoblasts (L6-cells). RESULTS AND CONCLUSIONS: Extracts of Artemisia roxburghiana, Roylea cinerea, Leucas cephalotes, Nepeta hindostana and Viola canescens showed good antiplasmodial activity (IC50<5 μg/ml). The chloroform extract of Artemisia roxburghiana was the most active (IC50 value of 0.42 μg/ml) and the most selective (SI=78) extract for Plasmodium falciparum among all plants extracts examined. The chloroform extract of Leucas cephalotes and the petroleum ether extract of Viola canescens exhibited substantial activities against Leishmania donovani with IC50 values of 3.61 μg/ml (SI=8) and 0.40 μg/ml (SI=30), respectively. The petroleum ether extract of Viola canescens exhibited activity against Trypanosoma cruzi with an IC50 value of 1.86 μg/ml (SI=7). Methanol and water extracts from all plants under investigation were found inactive against all parasites tested. These results support investigation of components of traditional medicines as potential new antiprotozoal agents. On the other hand since herbalism has become the main stream throughout the world, investigation demonstrates that these non-polar plant extracts of six of the plants examined in this study could play an important role in herbal formulations for the treatment of vector borne protozoal diseases. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21527328/Antiprotozoal_activities_of_traditional_medicinal_plants_from_the_Garhwal_region_of_North_West_Himalaya_India_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00264-9 DB - PRIME DP - Unbound Medicine ER -