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Mechanisms underlying the protective effect of zinc and selenium against cadmium-induced oxidative stress in zebrafish Danio rerio.
Biometals. 2011 Dec; 24(6):981-92.B

Abstract

The present study was designed to elucidate the protective effect mechanism of Zinc (Zn) and Selenium (Se) on cadmium (Cd)-induced oxidative stress in zebrafish. For this purpose we investigate the response of oxidative stress markers, metallothionein accumulation and gene expression in liver and ovary of female zebrafish exposed to 0,4 mg/l Cd in water and supplemented with Zn (5 mg kg(-1)) and/or Se (2 mg kg(-1)) for 21 days in their diet. Liver and ovary Cd uptake was evaluated after the exposure period. Cd exposure significantly inhibited the antioxidant enzyme activities termed as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxydase (GPx) and caused a pronounced malondialdehyde (MDA) accumulation in both organs. Co-administration of Zn and Se reversed the Cd-induced toxicity in liver and ovary measured as MDA accumulation. Interestingly, gene expression patterns of Cat, CuZnSod and Gpx were up-regulated when related enzymatic activities were altered. Zebrafish metallothionein transcripts (zMt) significantly decreased in tissues of fish supplemented with Zn and/or Se when compared to Cd-exposed fish. Our data would suggest that Zn and Se protective mechanism against Cd-induced oxidative stress is more depending on the correction of the proteins biological activities rather than on the transcriptional level of related genes.

Authors+Show Affiliations

Laboratoire de Biochimie et Toxicologie de l'Environnement ISA, Chott-Mariem, Sousse, Tunisia. m_banni@yahoo.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21533903

Citation

Banni, Mohamed, et al. "Mechanisms Underlying the Protective Effect of Zinc and Selenium Against Cadmium-induced Oxidative Stress in Zebrafish Danio Rerio." Biometals : an International Journal On the Role of Metal Ions in Biology, Biochemistry, and Medicine, vol. 24, no. 6, 2011, pp. 981-92.
Banni M, Chouchene L, Said K, et al. Mechanisms underlying the protective effect of zinc and selenium against cadmium-induced oxidative stress in zebrafish Danio rerio. Biometals. 2011;24(6):981-92.
Banni, M., Chouchene, L., Said, K., Kerkeni, A., & Messaoudi, I. (2011). Mechanisms underlying the protective effect of zinc and selenium against cadmium-induced oxidative stress in zebrafish Danio rerio. Biometals : an International Journal On the Role of Metal Ions in Biology, Biochemistry, and Medicine, 24(6), 981-92. https://doi.org/10.1007/s10534-011-9456-z
Banni M, et al. Mechanisms Underlying the Protective Effect of Zinc and Selenium Against Cadmium-induced Oxidative Stress in Zebrafish Danio Rerio. Biometals. 2011;24(6):981-92. PubMed PMID: 21533903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanisms underlying the protective effect of zinc and selenium against cadmium-induced oxidative stress in zebrafish Danio rerio. AU - Banni,Mohamed, AU - Chouchene,Lina, AU - Said,Khaled, AU - Kerkeni,Abdelhamid, AU - Messaoudi,Imed, Y1 - 2011/05/01/ PY - 2010/07/29/received PY - 2011/04/20/accepted PY - 2011/5/3/entrez PY - 2011/5/3/pubmed PY - 2012/3/10/medline SP - 981 EP - 92 JF - Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine JO - Biometals VL - 24 IS - 6 N2 - The present study was designed to elucidate the protective effect mechanism of Zinc (Zn) and Selenium (Se) on cadmium (Cd)-induced oxidative stress in zebrafish. For this purpose we investigate the response of oxidative stress markers, metallothionein accumulation and gene expression in liver and ovary of female zebrafish exposed to 0,4 mg/l Cd in water and supplemented with Zn (5 mg kg(-1)) and/or Se (2 mg kg(-1)) for 21 days in their diet. Liver and ovary Cd uptake was evaluated after the exposure period. Cd exposure significantly inhibited the antioxidant enzyme activities termed as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxydase (GPx) and caused a pronounced malondialdehyde (MDA) accumulation in both organs. Co-administration of Zn and Se reversed the Cd-induced toxicity in liver and ovary measured as MDA accumulation. Interestingly, gene expression patterns of Cat, CuZnSod and Gpx were up-regulated when related enzymatic activities were altered. Zebrafish metallothionein transcripts (zMt) significantly decreased in tissues of fish supplemented with Zn and/or Se when compared to Cd-exposed fish. Our data would suggest that Zn and Se protective mechanism against Cd-induced oxidative stress is more depending on the correction of the proteins biological activities rather than on the transcriptional level of related genes. SN - 1572-8773 UR - https://www.unboundmedicine.com/medline/citation/21533903/Mechanisms_underlying_the_protective_effect_of_zinc_and_selenium_against_cadmium_induced_oxidative_stress_in_zebrafish_Danio_rerio_ L2 - https://doi.org/10.1007/s10534-011-9456-z DB - PRIME DP - Unbound Medicine ER -