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Activity-guided isolation and identification of radical scavenging components in Gao-Cha tea.
J Food Sci. 2010 Oct; 75(8):H239-43.JF

Abstract

Gao-Cha is a traditional Chinese health tea made from Acer ginnala. We performed a components and radical scavenging activity analysis to identify any medicinal components in this tea. High performance thin layer chromatography (HPTLC)-1,1-Diphenyl-2-picryhydrazyl (HPTLC-DPPH) assay showed that the methanolic extract contained strong radical scavengers. Quantitative analysis revealed that the IC(50) of the extract against 1 mM DPPH was 52.7 ± 0.6 μg/mL. Bioactive-guided isolations led to procurement of 3 radical scavengers with IC(50)s of 17.5 ± 2.1, 29.3 ± 2.5, and 21.6 ± 1.7 μg/mL, respectively. Analysis of the high resolution-electric spray ionization-mass spectrometer and (1)H, (13)C, distortionless enhancement by polarization transfer at 135°, heteronuclear quantum coherence, correlating spectroscopy coupling, and heteronuclear multiple bond coherence (HMBC) data revealed that the compounds were methyl 3, 4, 5-trihydroxybenzoate (1), quercetin-3-O-α-rhamnopyranoside (2), and 2,6-bis (3,4,5-trihydroxybenzoyl)-aceritol (3). Bioactive combined components analysis revealed that, apart from compounds 1, 2, and 3, the tea possibly contained radical scavengers: ginnalin A (4) and B (5), 2″-O-Galloylquercitrin (6) and 3″-O-Galloyl-quercitrin (7). Compounds 2, 6, and 7 were isolated from Acer ginnala for the first time. The positions of the 2 galloyl moieties in compound 3 were unambiguously established by the HMBC spectrum for the first time.

Authors+Show Affiliations

Anhui Agricultural Univ., Hefei 230036, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21535501

Citation

Lu, Rui-Li, et al. "Activity-guided Isolation and Identification of Radical Scavenging Components in Gao-Cha Tea." Journal of Food Science, vol. 75, no. 8, 2010, pp. H239-43.
Lu RL, Hu FL, Xia T. Activity-guided isolation and identification of radical scavenging components in Gao-Cha tea. J Food Sci. 2010;75(8):H239-43.
Lu, R. L., Hu, F. L., & Xia, T. (2010). Activity-guided isolation and identification of radical scavenging components in Gao-Cha tea. Journal of Food Science, 75(8), H239-43. https://doi.org/10.1111/j.1750-3841.2010.01804.x
Lu RL, Hu FL, Xia T. Activity-guided Isolation and Identification of Radical Scavenging Components in Gao-Cha Tea. J Food Sci. 2010;75(8):H239-43. PubMed PMID: 21535501.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activity-guided isolation and identification of radical scavenging components in Gao-Cha tea. AU - Lu,Rui-Li, AU - Hu,Feng-Lin, AU - Xia,Tao, Y1 - 2010/09/24/ PY - 2011/5/4/entrez PY - 2011/5/4/pubmed PY - 2011/8/24/medline SP - H239 EP - 43 JF - Journal of food science JO - J Food Sci VL - 75 IS - 8 N2 - Gao-Cha is a traditional Chinese health tea made from Acer ginnala. We performed a components and radical scavenging activity analysis to identify any medicinal components in this tea. High performance thin layer chromatography (HPTLC)-1,1-Diphenyl-2-picryhydrazyl (HPTLC-DPPH) assay showed that the methanolic extract contained strong radical scavengers. Quantitative analysis revealed that the IC(50) of the extract against 1 mM DPPH was 52.7 ± 0.6 μg/mL. Bioactive-guided isolations led to procurement of 3 radical scavengers with IC(50)s of 17.5 ± 2.1, 29.3 ± 2.5, and 21.6 ± 1.7 μg/mL, respectively. Analysis of the high resolution-electric spray ionization-mass spectrometer and (1)H, (13)C, distortionless enhancement by polarization transfer at 135°, heteronuclear quantum coherence, correlating spectroscopy coupling, and heteronuclear multiple bond coherence (HMBC) data revealed that the compounds were methyl 3, 4, 5-trihydroxybenzoate (1), quercetin-3-O-α-rhamnopyranoside (2), and 2,6-bis (3,4,5-trihydroxybenzoyl)-aceritol (3). Bioactive combined components analysis revealed that, apart from compounds 1, 2, and 3, the tea possibly contained radical scavengers: ginnalin A (4) and B (5), 2″-O-Galloylquercitrin (6) and 3″-O-Galloyl-quercitrin (7). Compounds 2, 6, and 7 were isolated from Acer ginnala for the first time. The positions of the 2 galloyl moieties in compound 3 were unambiguously established by the HMBC spectrum for the first time. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/21535501/Activity_guided_isolation_and_identification_of_radical_scavenging_components_in_Gao_Cha_tea_ L2 - https://doi.org/10.1111/j.1750-3841.2010.01804.x DB - PRIME DP - Unbound Medicine ER -