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Structural characterization and antioxidant activities of 2 water-soluble polysaccharide fractions purified from tea (Camellia sinensis) flower.
J Food Sci. 2011 Apr; 76(3):C462-71.JF

Abstract

The water-soluble crude polysaccharide tea flower polysaccharide (TFP), obtained from tea (Camellia sinensis) flower by boiling-water extraction and ethanol precipitation, was fractionated by Sephadex G-100 column chromatography, giving 2 polysaccharide fractions termed TFP-1 and TFP-2. The structural features of TFP-1 and TFP-2 were investigated by high-performance liquid chromatography (HPLC), gel-permeation chromatography (GPC), rheometer, infrared (IR) spectra, nuclear magnetic resonance (NMR) spectroscopy, atomic force microscope (AFM), and scanning electron microscope (SEM). Results indicated that TFP-1 was composed of glucose: xylose: rhamnose: galactose=1.0:1.2:0.81:0.98 with a molecular weight of 167.5 KDa, while TFP-2 comprised glucose: xylose: rhamnose: arabinose=1.0:0.76:2.3:2.3 with a molecular weight of 10.1 KDa. The 1H NMR revealed that TFP-1 contained α-L-Rhap, α-D-Galp, α-D-GalpNAc, α-D-Xylp, α-D-Glcp, and β-D-Glcp residues, while TFP-2 was illustrated to have α-L-Rhap, α-L-Arap, α-D-Xylp, α-D-Glcp, and α-D-GlcpNAc residues. Antioxidant activities of these fractions were investigated using various in vitro assay systems compared with ascorbic acid. In conclusion, TFP-2 exhibited the higher antioxidant activities and could be explored as a novel potential antioxidant.

PRACTICAL APPLICATION

At present, commonly low-grade tea is preferred to extract the tea polysaccharide, to take full advantage of tea flower resource to extract polysaccharides can greatly reduce the cost of tea products. Thus, the search for plant-derived biomaterials from this study could generate natural value-added products from underutilized tea plant waste and used as a medicinal agent against chronic health problems, such as cancers, aging, and atherosclerosis caused by reactive free radicals that produced from oxidation.

Authors+Show Affiliations

Department of Tea Science, Zhejiang University, Hangzhou 310029, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

21535815

Citation

Quan, Han, et al. "Structural Characterization and Antioxidant Activities of 2 Water-soluble Polysaccharide Fractions Purified From Tea (Camellia Sinensis) Flower." Journal of Food Science, vol. 76, no. 3, 2011, pp. C462-71.
Quan H, Qiong-Yao Y, Jiang S, et al. Structural characterization and antioxidant activities of 2 water-soluble polysaccharide fractions purified from tea (Camellia sinensis) flower. J Food Sci. 2011;76(3):C462-71.
Quan, H., Qiong-Yao, Y., Jiang, S., Chang-Yun, X., Ze-Jie, L., & Pu-Ming, H. (2011). Structural characterization and antioxidant activities of 2 water-soluble polysaccharide fractions purified from tea (Camellia sinensis) flower. Journal of Food Science, 76(3), C462-71. https://doi.org/10.1111/j.1750-3841.2011.02063.x
Quan H, et al. Structural Characterization and Antioxidant Activities of 2 Water-soluble Polysaccharide Fractions Purified From Tea (Camellia Sinensis) Flower. J Food Sci. 2011;76(3):C462-71. PubMed PMID: 21535815.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural characterization and antioxidant activities of 2 water-soluble polysaccharide fractions purified from tea (Camellia sinensis) flower. AU - Quan,Han, AU - Qiong-Yao,Yu, AU - Jiang,Shi, AU - Chang-Yun,Xiong, AU - Ze-Jie,Ling, AU - Pu-Ming,He, Y1 - 2011/03/16/ PY - 2011/5/4/entrez PY - 2011/5/4/pubmed PY - 2011/8/27/medline SP - C462 EP - 71 JF - Journal of food science JO - J Food Sci VL - 76 IS - 3 N2 - UNLABELLED: The water-soluble crude polysaccharide tea flower polysaccharide (TFP), obtained from tea (Camellia sinensis) flower by boiling-water extraction and ethanol precipitation, was fractionated by Sephadex G-100 column chromatography, giving 2 polysaccharide fractions termed TFP-1 and TFP-2. The structural features of TFP-1 and TFP-2 were investigated by high-performance liquid chromatography (HPLC), gel-permeation chromatography (GPC), rheometer, infrared (IR) spectra, nuclear magnetic resonance (NMR) spectroscopy, atomic force microscope (AFM), and scanning electron microscope (SEM). Results indicated that TFP-1 was composed of glucose: xylose: rhamnose: galactose=1.0:1.2:0.81:0.98 with a molecular weight of 167.5 KDa, while TFP-2 comprised glucose: xylose: rhamnose: arabinose=1.0:0.76:2.3:2.3 with a molecular weight of 10.1 KDa. The 1H NMR revealed that TFP-1 contained α-L-Rhap, α-D-Galp, α-D-GalpNAc, α-D-Xylp, α-D-Glcp, and β-D-Glcp residues, while TFP-2 was illustrated to have α-L-Rhap, α-L-Arap, α-D-Xylp, α-D-Glcp, and α-D-GlcpNAc residues. Antioxidant activities of these fractions were investigated using various in vitro assay systems compared with ascorbic acid. In conclusion, TFP-2 exhibited the higher antioxidant activities and could be explored as a novel potential antioxidant. PRACTICAL APPLICATION: At present, commonly low-grade tea is preferred to extract the tea polysaccharide, to take full advantage of tea flower resource to extract polysaccharides can greatly reduce the cost of tea products. Thus, the search for plant-derived biomaterials from this study could generate natural value-added products from underutilized tea plant waste and used as a medicinal agent against chronic health problems, such as cancers, aging, and atherosclerosis caused by reactive free radicals that produced from oxidation. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/21535815/Structural_characterization_and_antioxidant_activities_of_2_water_soluble_polysaccharide_fractions_purified_from_tea__Camellia_sinensis__flower_ L2 - https://doi.org/10.1111/j.1750-3841.2011.02063.x DB - PRIME DP - Unbound Medicine ER -