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Hydroxysafflor yellow A protects against chronic carbon tetrachloride-induced liver fibrosis.
Eur J Pharmacol 2011; 660(2-3):438-44EJ

Abstract

Hydroxysafflor yellow A (HSYA) was isolated from the dried flower of Carthamus tinctorius L. which was extensively used in traditional Chinese medicine to treat cirrhosis. However, the potential protective effect of HSYA in liver fibrosis is still unknown. In the present study, we investigated the effects of HSYA in rats with carbon tetrachloride (CCl4)-induced liver fibrosis. Sprague-Dawley (SD) rats were subjected to biweekly CCl4 injections over 12 weeks, while controls were given isovolumetric injections of olive oil. HSYA was given in a daily dose of 5 mg/kg by means of intraperitoneal concurrent with CCl4. Hepatic fibrosis was quantified by digital analysis of Masson's trichrome stained slides and hydroxyproline content. mRNA expression was quantified by real-time polymerase chain reaction (PCR), and protein was quantified by western blot or enzyme-linked immunosorbent assay (ELISA). CCl4 treatment induced micronodular liver fibrosis with a pronounced deposition of collagen fibers. HSYA significantly reduced liver fibrosis. HSYA down regulates α-smooth muscle actin (SMA), collagen α type I, matrix metalloproteinases (MMP)-9, and tissue inhibitors of metalloproteinases (TIMP)-1 gene expression. This was accompanied by a decreased expression of transforming growth factor (TGF)-β1 and phosphorylation of Smad4. These results indicate that HSYA might be a promising antifibrotic agent in chronic liver disease.

Authors+Show Affiliations

The Institute of Medicine, Qiqihar Medical University, Qiqihar 161006, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21536026

Citation

Zhang, Yingbo, et al. "Hydroxysafflor Yellow a Protects Against Chronic Carbon Tetrachloride-induced Liver Fibrosis." European Journal of Pharmacology, vol. 660, no. 2-3, 2011, pp. 438-44.
Zhang Y, Guo J, Dong H, et al. Hydroxysafflor yellow A protects against chronic carbon tetrachloride-induced liver fibrosis. Eur J Pharmacol. 2011;660(2-3):438-44.
Zhang, Y., Guo, J., Dong, H., Zhao, X., Zhou, L., Li, X., ... Niu, Y. (2011). Hydroxysafflor yellow A protects against chronic carbon tetrachloride-induced liver fibrosis. European Journal of Pharmacology, 660(2-3), pp. 438-44. doi:10.1016/j.ejphar.2011.04.015.
Zhang Y, et al. Hydroxysafflor Yellow a Protects Against Chronic Carbon Tetrachloride-induced Liver Fibrosis. Eur J Pharmacol. 2011 Jun 25;660(2-3):438-44. PubMed PMID: 21536026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydroxysafflor yellow A protects against chronic carbon tetrachloride-induced liver fibrosis. AU - Zhang,Yingbo, AU - Guo,Jia, AU - Dong,Hanying, AU - Zhao,Xuemei, AU - Zhou,Li, AU - Li,Xueyan, AU - Liu,Jicheng, AU - Niu,Yingcai, Y1 - 2011/04/27/ PY - 2010/11/25/received PY - 2011/04/04/revised PY - 2011/04/12/accepted PY - 2011/5/4/entrez PY - 2011/5/4/pubmed PY - 2011/9/7/medline SP - 438 EP - 44 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 660 IS - 2-3 N2 - Hydroxysafflor yellow A (HSYA) was isolated from the dried flower of Carthamus tinctorius L. which was extensively used in traditional Chinese medicine to treat cirrhosis. However, the potential protective effect of HSYA in liver fibrosis is still unknown. In the present study, we investigated the effects of HSYA in rats with carbon tetrachloride (CCl4)-induced liver fibrosis. Sprague-Dawley (SD) rats were subjected to biweekly CCl4 injections over 12 weeks, while controls were given isovolumetric injections of olive oil. HSYA was given in a daily dose of 5 mg/kg by means of intraperitoneal concurrent with CCl4. Hepatic fibrosis was quantified by digital analysis of Masson's trichrome stained slides and hydroxyproline content. mRNA expression was quantified by real-time polymerase chain reaction (PCR), and protein was quantified by western blot or enzyme-linked immunosorbent assay (ELISA). CCl4 treatment induced micronodular liver fibrosis with a pronounced deposition of collagen fibers. HSYA significantly reduced liver fibrosis. HSYA down regulates α-smooth muscle actin (SMA), collagen α type I, matrix metalloproteinases (MMP)-9, and tissue inhibitors of metalloproteinases (TIMP)-1 gene expression. This was accompanied by a decreased expression of transforming growth factor (TGF)-β1 and phosphorylation of Smad4. These results indicate that HSYA might be a promising antifibrotic agent in chronic liver disease. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/21536026/Hydroxysafflor_yellow_A_protects_against_chronic_carbon_tetrachloride_induced_liver_fibrosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(11)00405-5 DB - PRIME DP - Unbound Medicine ER -