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Detection of the synthetic drug 4-fluoroamphetamine (4-FA) in serum and urine.
Forensic Sci Int. 2012 Feb 10; 215(1-3):3-7.FS

Abstract

4-Fluoroamphetamine (4-FA) was detected in the blood and urine of two individuals suspected for driving under the influence (DUI). The test for amphetamines in urine subjected to immunoassay screening using the CEDIA DAU assay proved positive. Further investigations revealed a 4-FA cross-reactivity of about 6% in the CEDIA amphetamine assay. 4-FA was qualitatively detected in a general unknown screening for drugs using GC/MS in full scan mode. No other drugs or fluorinated phenethylamines were detected. A validated GC/MS method was established in SIM mode for serum analysis of 4-FA with a limit of detection (LOD) of 1 ng/mL and a lower limit of quantification (LLOQ) of 5 ng/mL. Intra-assay precision was approx. 4% and inter-assay precision approx. 8%. Applying this method, the 4-FA serum concentrations of the two subjects were determined to be 350 ng/mL and 475 ng/mL, respectively. Given the pharmacological data of amphetamine, 4-FA psychoactive effects are to be expected at these serum levels. Both subjects exhibited sympathomimetic effects and psychostimulant-like impairment accordingly.

Authors+Show Affiliations

Institute of Legal Medicine, Johannes Gutenberg-University Mainz, Am Pulverturm 3, 55131 Mainz, Germany. roehrich@uni-mainz.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

21543168

Citation

Röhrich, J, et al. "Detection of the Synthetic Drug 4-fluoroamphetamine (4-FA) in Serum and Urine." Forensic Science International, vol. 215, no. 1-3, 2012, pp. 3-7.
Röhrich J, Becker J, Kaufmann T, et al. Detection of the synthetic drug 4-fluoroamphetamine (4-FA) in serum and urine. Forensic Sci Int. 2012;215(1-3):3-7.
Röhrich, J., Becker, J., Kaufmann, T., Zörntlein, S., & Urban, R. (2012). Detection of the synthetic drug 4-fluoroamphetamine (4-FA) in serum and urine. Forensic Science International, 215(1-3), 3-7. https://doi.org/10.1016/j.forsciint.2011.04.004
Röhrich J, et al. Detection of the Synthetic Drug 4-fluoroamphetamine (4-FA) in Serum and Urine. Forensic Sci Int. 2012 Feb 10;215(1-3):3-7. PubMed PMID: 21543168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of the synthetic drug 4-fluoroamphetamine (4-FA) in serum and urine. AU - Röhrich,J, AU - Becker,J, AU - Kaufmann,T, AU - Zörntlein,S, AU - Urban,R, Y1 - 2011/05/04/ PY - 2010/09/19/received PY - 2011/03/30/revised PY - 2011/04/06/accepted PY - 2011/5/6/entrez PY - 2011/5/6/pubmed PY - 2012/6/5/medline SP - 3 EP - 7 JF - Forensic science international JO - Forensic Sci Int VL - 215 IS - 1-3 N2 - 4-Fluoroamphetamine (4-FA) was detected in the blood and urine of two individuals suspected for driving under the influence (DUI). The test for amphetamines in urine subjected to immunoassay screening using the CEDIA DAU assay proved positive. Further investigations revealed a 4-FA cross-reactivity of about 6% in the CEDIA amphetamine assay. 4-FA was qualitatively detected in a general unknown screening for drugs using GC/MS in full scan mode. No other drugs or fluorinated phenethylamines were detected. A validated GC/MS method was established in SIM mode for serum analysis of 4-FA with a limit of detection (LOD) of 1 ng/mL and a lower limit of quantification (LLOQ) of 5 ng/mL. Intra-assay precision was approx. 4% and inter-assay precision approx. 8%. Applying this method, the 4-FA serum concentrations of the two subjects were determined to be 350 ng/mL and 475 ng/mL, respectively. Given the pharmacological data of amphetamine, 4-FA psychoactive effects are to be expected at these serum levels. Both subjects exhibited sympathomimetic effects and psychostimulant-like impairment accordingly. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/21543168/Detection_of_the_synthetic_drug_4_fluoroamphetamine__4_FA__in_serum_and_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(11)00171-X DB - PRIME DP - Unbound Medicine ER -