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An update of the evolving epidemic of blaKPC-2-carrying Klebsiella pneumoniae in Greece (2009-10).
J Antimicrob Chemother. 2011 Jul; 66(7):1510-3.JA

Abstract

OBJECTIVES

To follow the epidemic of KPC-2-producing Klebsiella pneumoniae in Greece.

METHODS

KPC-2-producing isolates (n = 378) were collected during January 2009-April 2010 in 40 Greek hospitals. bla(KPC) and bla(VIM) were detected by PCR. Carbapenemase production was confirmed by spectrophotometry. Sequences flanking bla(KPC-2) and their plasmid carriers were studied. Isolates were typed by PFGE and multilocus sequence typing (MLST).

RESULTS

All 378 isolates were bla(KPC-2) positive; 18 also carried bla(VIM-1/VIM-4). Higher isolation frequencies were observed in Athens and Crete. Isolates were classified into 13 PFGE types and 11 sequence types (STs). ST258 was predominant (n = 322), followed by ST147 (n = 20), ST383 (n = 9), ST133 (n = 6), ST274 (n = 4) and ST323 (n = 3). Of the remaining isolates, seven were distributed into five STs (11, 17, 340 and the novel 494 and 495) and seven were not typed. bla(KPC-2) could not be transferred from ST258 isolates, in contrast to isolates of ST17, ST133, ST147, ST274, ST494 and ST495. All bla(KPC-2)-encoding plasmids were of similar size (∼100 kb) and showed indistinguishable restriction fragment length polymorphism (RFLP) patterns except those from the ST340 isolates. Sequences flanking bla(KPC-2) revealed that the Tn4401a isoform was present in plasmids from all STs except ST340 containing Tn4401b. Co-production of VIM enzymes was observed in isolates of ST147, ST323 and ST383.

CONCLUSIONS

Apart from the epidemic of KPC-2-producing K. pneumoniae belonging to ST258 in Greece, diffusion of bla(KPC-2) to at least 10 additional STs has taken place. Notably, strains from three of the latter STs (147, 323 and 383) were found to carry both bla(KPC-2) and bla(VIM).

Authors+Show Affiliations

Department of Microbiology, National School of Public Health, Athens, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21543359

Citation

Giakkoupi, Panagiota, et al. "An Update of the Evolving Epidemic of blaKPC-2-carrying Klebsiella Pneumoniae in Greece (2009-10)." The Journal of Antimicrobial Chemotherapy, vol. 66, no. 7, 2011, pp. 1510-3.
Giakkoupi P, Papagiannitsis CC, Miriagou V, et al. An update of the evolving epidemic of blaKPC-2-carrying Klebsiella pneumoniae in Greece (2009-10). J Antimicrob Chemother. 2011;66(7):1510-3.
Giakkoupi, P., Papagiannitsis, C. C., Miriagou, V., Pappa, O., Polemis, M., Tryfinopoulou, K., Tzouvelekis, L. S., & Vatopoulos, A. C. (2011). An update of the evolving epidemic of blaKPC-2-carrying Klebsiella pneumoniae in Greece (2009-10). The Journal of Antimicrobial Chemotherapy, 66(7), 1510-3. https://doi.org/10.1093/jac/dkr166
Giakkoupi P, et al. An Update of the Evolving Epidemic of blaKPC-2-carrying Klebsiella Pneumoniae in Greece (2009-10). J Antimicrob Chemother. 2011;66(7):1510-3. PubMed PMID: 21543359.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An update of the evolving epidemic of blaKPC-2-carrying Klebsiella pneumoniae in Greece (2009-10). AU - Giakkoupi,Panagiota, AU - Papagiannitsis,Costas C, AU - Miriagou,Vivi, AU - Pappa,Olga, AU - Polemis,Michalis, AU - Tryfinopoulou,Kyriaki, AU - Tzouvelekis,Leonidas S, AU - Vatopoulos,Alkiviadis C, Y1 - 2011/05/04/ PY - 2011/5/6/entrez PY - 2011/5/6/pubmed PY - 2011/9/29/medline SP - 1510 EP - 3 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 66 IS - 7 N2 - OBJECTIVES: To follow the epidemic of KPC-2-producing Klebsiella pneumoniae in Greece. METHODS: KPC-2-producing isolates (n = 378) were collected during January 2009-April 2010 in 40 Greek hospitals. bla(KPC) and bla(VIM) were detected by PCR. Carbapenemase production was confirmed by spectrophotometry. Sequences flanking bla(KPC-2) and their plasmid carriers were studied. Isolates were typed by PFGE and multilocus sequence typing (MLST). RESULTS: All 378 isolates were bla(KPC-2) positive; 18 also carried bla(VIM-1/VIM-4). Higher isolation frequencies were observed in Athens and Crete. Isolates were classified into 13 PFGE types and 11 sequence types (STs). ST258 was predominant (n = 322), followed by ST147 (n = 20), ST383 (n = 9), ST133 (n = 6), ST274 (n = 4) and ST323 (n = 3). Of the remaining isolates, seven were distributed into five STs (11, 17, 340 and the novel 494 and 495) and seven were not typed. bla(KPC-2) could not be transferred from ST258 isolates, in contrast to isolates of ST17, ST133, ST147, ST274, ST494 and ST495. All bla(KPC-2)-encoding plasmids were of similar size (∼100 kb) and showed indistinguishable restriction fragment length polymorphism (RFLP) patterns except those from the ST340 isolates. Sequences flanking bla(KPC-2) revealed that the Tn4401a isoform was present in plasmids from all STs except ST340 containing Tn4401b. Co-production of VIM enzymes was observed in isolates of ST147, ST323 and ST383. CONCLUSIONS: Apart from the epidemic of KPC-2-producing K. pneumoniae belonging to ST258 in Greece, diffusion of bla(KPC-2) to at least 10 additional STs has taken place. Notably, strains from three of the latter STs (147, 323 and 383) were found to carry both bla(KPC-2) and bla(VIM). SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/21543359/An_update_of_the_evolving_epidemic_of_blaKPC_2_carrying_Klebsiella_pneumoniae_in_Greece__2009_10__ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkr166 DB - PRIME DP - Unbound Medicine ER -