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Feasibility of rat hair as a quality control material for the determination of methamphetamine and amphetamine in human hair.
Arch Pharm Res. 2011 Apr; 34(4):593-8.AP

Abstract

A quality control material (QCM) is a necessity in hair drug analysis, but it is not always easy to have an authentic hair sample containing various target drugs and metabolites. In the present study, the feasibility of rat hair as a QCM was examined for its application in the determination of methamphetamine (MA) and amphetamine (AP) in human hair. MA was administered to lean Zucker rats, from which only pigmented hair was collected for the preparation of a QCM. The rat hair was then washed, homogenized and finally bottled. Both homogeneity and stability were examined in order to demonstrate the suitability of rat hair as a QCM in hair drug analysis. The concentrations of MA and AP in each bottle were determined using extraction with 1% HCl in methanol at 38°C followed by gas chromatography/mass spectrometry after derivatization with trifluoroacetic anhydride. Furthermore, the prepared QCM was used in an inter-laboratory quality assurance program. In the homogeneity test, no significant difference was observed between bottles of the QCM. The statistical results also showed no significant trends in stability for three months at room temperature. An inter-laboratory quality assurance program was also performed successfully using this material. Thus, rat hair will be useful as an alternative QCM sample for the determination of a variety of drugs and their metabolites in human hair.

Authors+Show Affiliations

National Forensic Service, Seoul, 158-707, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21544724

Citation

Lee, Sooyeun, et al. "Feasibility of Rat Hair as a Quality Control Material for the Determination of Methamphetamine and Amphetamine in Human Hair." Archives of Pharmacal Research, vol. 34, no. 4, 2011, pp. 593-8.
Lee S, Park Y, Kim J, et al. Feasibility of rat hair as a quality control material for the determination of methamphetamine and amphetamine in human hair. Arch Pharm Res. 2011;34(4):593-8.
Lee, S., Park, Y., Kim, J., In, S., Choi, H., Chung, H., Oh, S. M., & Chung, K. H. (2011). Feasibility of rat hair as a quality control material for the determination of methamphetamine and amphetamine in human hair. Archives of Pharmacal Research, 34(4), 593-8. https://doi.org/10.1007/s12272-011-0409-4
Lee S, et al. Feasibility of Rat Hair as a Quality Control Material for the Determination of Methamphetamine and Amphetamine in Human Hair. Arch Pharm Res. 2011;34(4):593-8. PubMed PMID: 21544724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Feasibility of rat hair as a quality control material for the determination of methamphetamine and amphetamine in human hair. AU - Lee,Sooyeun, AU - Park,Yonghoon, AU - Kim,Jihyun, AU - In,Sanghwan, AU - Choi,Hwakyung, AU - Chung,Heesun, AU - Oh,Seung Min, AU - Chung,Kyu Hyuck, Y1 - 2011/05/05/ PY - 2010/11/23/received PY - 2010/12/29/accepted PY - 2010/12/28/revised PY - 2011/5/6/entrez PY - 2011/5/6/pubmed PY - 2011/9/13/medline SP - 593 EP - 8 JF - Archives of pharmacal research JO - Arch Pharm Res VL - 34 IS - 4 N2 - A quality control material (QCM) is a necessity in hair drug analysis, but it is not always easy to have an authentic hair sample containing various target drugs and metabolites. In the present study, the feasibility of rat hair as a QCM was examined for its application in the determination of methamphetamine (MA) and amphetamine (AP) in human hair. MA was administered to lean Zucker rats, from which only pigmented hair was collected for the preparation of a QCM. The rat hair was then washed, homogenized and finally bottled. Both homogeneity and stability were examined in order to demonstrate the suitability of rat hair as a QCM in hair drug analysis. The concentrations of MA and AP in each bottle were determined using extraction with 1% HCl in methanol at 38°C followed by gas chromatography/mass spectrometry after derivatization with trifluoroacetic anhydride. Furthermore, the prepared QCM was used in an inter-laboratory quality assurance program. In the homogeneity test, no significant difference was observed between bottles of the QCM. The statistical results also showed no significant trends in stability for three months at room temperature. An inter-laboratory quality assurance program was also performed successfully using this material. Thus, rat hair will be useful as an alternative QCM sample for the determination of a variety of drugs and their metabolites in human hair. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/21544724/Feasibility_of_rat_hair_as_a_quality_control_material_for_the_determination_of_methamphetamine_and_amphetamine_in_human_hair_ L2 - https://dx.doi.org/10.1007/s12272-011-0409-4 DB - PRIME DP - Unbound Medicine ER -