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A protocol for the evaluation of genotoxicity in bile of carp (Cyprinus carpio) exposed to lake water treated with different disinfectants.
Chemosphere. 2011 Sep; 84(10):1521-6.C

Abstract

A sensitive and rapid method to evaluate toxic and genotoxic properties of drinking water supplied from Lake Trasimeno (Umbria, Central Italy) was worked out analysing bile in Cyprinus carpio exposed for 20 d to lake water treated with 3 different disinfectants, sodium hypochlorite (NaClO), chlorine dioxide (ClO(2)) and peracetic acid (PAA). Fish were sacrificed at 0, 10 and 20 d in order to investigate the time course of these endpoints. An aliquot of bile samples was fractionated by adsorption on C(18) silica cartridges and the genotoxic potential of whole bile and of bile fractions was evaluated by the single-cell microgel-electrophoresis (comet) assay on human colonic adenocarcinoma cells (Caco-2). Bile (both whole and fractionated) from specimens exposed to the three disinfectants always showed a genotoxic activity as compared to the control group. The results of this study provide evidence that all three disinfectants cause an increase in bile genotoxicity of chronically exposed fish.

Authors+Show Affiliations

Department of Medical-Surgical Specialties and Public Health, University of Perugia, Via del Giochetto, I-06122 Perugia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21546055

Citation

Villarini, Milena, et al. "A Protocol for the Evaluation of Genotoxicity in Bile of Carp (Cyprinus Carpio) Exposed to Lake Water Treated With Different Disinfectants." Chemosphere, vol. 84, no. 10, 2011, pp. 1521-6.
Villarini M, Moretti M, Dominici L, et al. A protocol for the evaluation of genotoxicity in bile of carp (Cyprinus carpio) exposed to lake water treated with different disinfectants. Chemosphere. 2011;84(10):1521-6.
Villarini, M., Moretti, M., Dominici, L., Fatigoni, C., Dörr, A. J., Elia, A. C., & Monarca, S. (2011). A protocol for the evaluation of genotoxicity in bile of carp (Cyprinus carpio) exposed to lake water treated with different disinfectants. Chemosphere, 84(10), 1521-6. https://doi.org/10.1016/j.chemosphere.2011.04.031
Villarini M, et al. A Protocol for the Evaluation of Genotoxicity in Bile of Carp (Cyprinus Carpio) Exposed to Lake Water Treated With Different Disinfectants. Chemosphere. 2011;84(10):1521-6. PubMed PMID: 21546055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A protocol for the evaluation of genotoxicity in bile of carp (Cyprinus carpio) exposed to lake water treated with different disinfectants. AU - Villarini,Milena, AU - Moretti,Massimo, AU - Dominici,Luca, AU - Fatigoni,Cristina, AU - Dörr,Ambrosius Josef Martin, AU - Elia,Antonia Concetta, AU - Monarca,Silvano, Y1 - 2011/05/04/ PY - 2010/11/22/received PY - 2011/03/31/revised PY - 2011/04/07/accepted PY - 2011/5/7/entrez PY - 2011/5/7/pubmed PY - 2011/12/13/medline SP - 1521 EP - 6 JF - Chemosphere JO - Chemosphere VL - 84 IS - 10 N2 - A sensitive and rapid method to evaluate toxic and genotoxic properties of drinking water supplied from Lake Trasimeno (Umbria, Central Italy) was worked out analysing bile in Cyprinus carpio exposed for 20 d to lake water treated with 3 different disinfectants, sodium hypochlorite (NaClO), chlorine dioxide (ClO(2)) and peracetic acid (PAA). Fish were sacrificed at 0, 10 and 20 d in order to investigate the time course of these endpoints. An aliquot of bile samples was fractionated by adsorption on C(18) silica cartridges and the genotoxic potential of whole bile and of bile fractions was evaluated by the single-cell microgel-electrophoresis (comet) assay on human colonic adenocarcinoma cells (Caco-2). Bile (both whole and fractionated) from specimens exposed to the three disinfectants always showed a genotoxic activity as compared to the control group. The results of this study provide evidence that all three disinfectants cause an increase in bile genotoxicity of chronically exposed fish. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/21546055/A_protocol_for_the_evaluation_of_genotoxicity_in_bile_of_carp__Cyprinus_carpio__exposed_to_lake_water_treated_with_different_disinfectants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(11)00422-X DB - PRIME DP - Unbound Medicine ER -