Tags

Type your tag names separated by a space and hit enter

Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation.
Biosens Bioelectron 2011; 26(10):3993-4000BB

Abstract

Poly(ethylene glycol) diglycidyl ether (PEGDE) is widely used as an additive for cross-linking polymers bearing amine, hydroxyl, or carboxyl groups. However, the idea of using PEGDE alone for immobilizing proteins on biosensors has never been thoroughly explored. We report the successful fabrication of microelectrode biosensors based on glucose oxidase, d-amino acid oxidase, and glutamate oxidase immobilized using PEGDE. We found that biosensors made with PEGDE exhibited high sensitivity and a response time on the order of seconds, which is sufficient for observing biological processes in vivo. The enzymatic activity on these biosensors was highly stable over several months when they were stored at 4 °C, and over at least 3d at 37 °C. Glucose microelectrode biosensors implanted in the central nervous system of anesthetized rats reliably monitored changes in brain glucose levels induced by sequential administration of insulin and glucose. PEGDE provides a simple, low cost, non-toxic alternative for the preparation of in vivo microelectrode biosensors.

Authors+Show Affiliations

INSERM U1028, CNRS UMR5292, Lyon Neuroscience Research Center, Integrated Physiology of the Waking State, Lyon, F-69000, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

21546239

Citation

Vasylieva, Natalia, et al. "Covalent Enzyme Immobilization By Poly(ethylene Glycol) Diglycidyl Ether (PEGDE) for Microelectrode Biosensor Preparation." Biosensors & Bioelectronics, vol. 26, no. 10, 2011, pp. 3993-4000.
Vasylieva N, Barnych B, Meiller A, et al. Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation. Biosens Bioelectron. 2011;26(10):3993-4000.
Vasylieva, N., Barnych, B., Meiller, A., Maucler, C., Pollegioni, L., Lin, J. S., ... Marinesco, S. (2011). Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation. Biosensors & Bioelectronics, 26(10), pp. 3993-4000. doi:10.1016/j.bios.2011.03.012.
Vasylieva N, et al. Covalent Enzyme Immobilization By Poly(ethylene Glycol) Diglycidyl Ether (PEGDE) for Microelectrode Biosensor Preparation. Biosens Bioelectron. 2011 Jun 15;26(10):3993-4000. PubMed PMID: 21546239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation. AU - Vasylieva,Natalia, AU - Barnych,Bogdan, AU - Meiller,Anne, AU - Maucler,Caroline, AU - Pollegioni,Loredano, AU - Lin,Jian-Sheng, AU - Barbier,Daniel, AU - Marinesco,Stéphane, Y1 - 2011/04/06/ PY - 2011/01/05/received PY - 2011/03/04/revised PY - 2011/03/11/accepted PY - 2011/5/7/entrez PY - 2011/5/7/pubmed PY - 2011/9/29/medline SP - 3993 EP - 4000 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 10 N2 - Poly(ethylene glycol) diglycidyl ether (PEGDE) is widely used as an additive for cross-linking polymers bearing amine, hydroxyl, or carboxyl groups. However, the idea of using PEGDE alone for immobilizing proteins on biosensors has never been thoroughly explored. We report the successful fabrication of microelectrode biosensors based on glucose oxidase, d-amino acid oxidase, and glutamate oxidase immobilized using PEGDE. We found that biosensors made with PEGDE exhibited high sensitivity and a response time on the order of seconds, which is sufficient for observing biological processes in vivo. The enzymatic activity on these biosensors was highly stable over several months when they were stored at 4 °C, and over at least 3d at 37 °C. Glucose microelectrode biosensors implanted in the central nervous system of anesthetized rats reliably monitored changes in brain glucose levels induced by sequential administration of insulin and glucose. PEGDE provides a simple, low cost, non-toxic alternative for the preparation of in vivo microelectrode biosensors. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/21546239/Covalent_enzyme_immobilization_by_poly_ethylene_glycol__diglycidyl_ether__PEGDE__for_microelectrode_biosensor_preparation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(11)00168-0 DB - PRIME DP - Unbound Medicine ER -