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Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells.
J Ethnopharmacol. 2011 Jun 14; 136(1):197-203.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

A medicinal herb Tribulus terrestris Linn has been used to treat various diseases including hepatocellular carcinoma. The aim of the present study was to investigate the anticancer activity of Tribulus terrestris Linn (TT) in liver cancer cells.

MATERIALS AND METHODS

The antitumor activity of aqueous TT extract was analyzed by testing the cytotoxicity and the effect on clonogenecity in HepG2 cells. Apoptosis and cell cycle arrest induced by TT were dissected by flow cytometry and its inhibitory effect on NF-κB activity was determined by analyzing the expression levels of NF-κB/IκB subunit proteins. The suppression of NF-κB-regulated gene expression by TT was assessed by RT-PCR.

RESULTS

TT extract repressed clonogenecity and proliferation, induced apoptosis, and enhanced accumulation in the G0/G1 phase of liver cancer cells. It also turned out that TT extract inhibited NF-κB-dependent reporter gene expression and NF-κB subunit p50 expression, while it enhanced the cellular level of IκBα by inhibiting the phosphorylation and degradation of IκBα. In addition, IKK activity was inhibited in a dose-dependent manner. Furthermore, TT extract suppressed the transcription of genes associated with cell cycle regulation, anti-apoptosis, and invasion.

CONCLUSION

These data showed that TT extract blocks proliferation and induces apoptosis in human liver cancer cells through the inhibition of NF-κB signaling. Aqueous TT extract can be used as an anticancer drug for hepatocellular carcinoma patients.

Authors+Show Affiliations

Department of Microbiology, Chungnam National University, Daejeon 305-764, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21549825

Citation

Kim, Hye Jin, et al. "Aqueous Extract of Tribulus Terrestris Linn Induces Cell Growth Arrest and Apoptosis By Down-regulating NF-κB Signaling in Liver Cancer Cells." Journal of Ethnopharmacology, vol. 136, no. 1, 2011, pp. 197-203.
Kim HJ, Kim JC, Min JS, et al. Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells. J Ethnopharmacol. 2011;136(1):197-203.
Kim, H. J., Kim, J. C., Min, J. S., Kim, M. J., Kim, J. A., Kor, M. H., Yoo, H. S., & Ahn, J. K. (2011). Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells. Journal of Ethnopharmacology, 136(1), 197-203. https://doi.org/10.1016/j.jep.2011.04.060
Kim HJ, et al. Aqueous Extract of Tribulus Terrestris Linn Induces Cell Growth Arrest and Apoptosis By Down-regulating NF-κB Signaling in Liver Cancer Cells. J Ethnopharmacol. 2011 Jun 14;136(1):197-203. PubMed PMID: 21549825.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aqueous extract of Tribulus terrestris Linn induces cell growth arrest and apoptosis by down-regulating NF-κB signaling in liver cancer cells. AU - Kim,Hye Jin, AU - Kim,Jin Chul, AU - Min,Jung Sun, AU - Kim,Mi-Jee, AU - Kim,Ji Ae, AU - Kor,Myung Ho, AU - Yoo,Hwa Seung, AU - Ahn,Jeong Keun, Y1 - 2011/04/28/ PY - 2010/11/01/received PY - 2011/03/31/revised PY - 2011/04/21/accepted PY - 2011/5/10/entrez PY - 2011/5/10/pubmed PY - 2011/12/31/medline SP - 197 EP - 203 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 136 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: A medicinal herb Tribulus terrestris Linn has been used to treat various diseases including hepatocellular carcinoma. The aim of the present study was to investigate the anticancer activity of Tribulus terrestris Linn (TT) in liver cancer cells. MATERIALS AND METHODS: The antitumor activity of aqueous TT extract was analyzed by testing the cytotoxicity and the effect on clonogenecity in HepG2 cells. Apoptosis and cell cycle arrest induced by TT were dissected by flow cytometry and its inhibitory effect on NF-κB activity was determined by analyzing the expression levels of NF-κB/IκB subunit proteins. The suppression of NF-κB-regulated gene expression by TT was assessed by RT-PCR. RESULTS: TT extract repressed clonogenecity and proliferation, induced apoptosis, and enhanced accumulation in the G0/G1 phase of liver cancer cells. It also turned out that TT extract inhibited NF-κB-dependent reporter gene expression and NF-κB subunit p50 expression, while it enhanced the cellular level of IκBα by inhibiting the phosphorylation and degradation of IκBα. In addition, IKK activity was inhibited in a dose-dependent manner. Furthermore, TT extract suppressed the transcription of genes associated with cell cycle regulation, anti-apoptosis, and invasion. CONCLUSION: These data showed that TT extract blocks proliferation and induces apoptosis in human liver cancer cells through the inhibition of NF-κB signaling. Aqueous TT extract can be used as an anticancer drug for hepatocellular carcinoma patients. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21549825/Aqueous_extract_of_Tribulus_terrestris_Linn_induces_cell_growth_arrest_and_apoptosis_by_down_regulating_NF_κB_signaling_in_liver_cancer_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00312-6 DB - PRIME DP - Unbound Medicine ER -