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Responses of a soil bacterium, Pseudomonas chlororaphis O6 to commercial metal oxide nanoparticles compared with responses to metal ions.
Environ Pollut. 2011 Jul; 159(7):1749-56.EP

Abstract

The toxicity of commercially-available CuO and ZnO nanoparticles (NPs) to pathogenic bacteria was compared for a beneficial rhizosphere isolate, Pseudomonas chlororaphis O6. The NPs aggregated, released ions to different extents under the conditions used for bacterial exposure, and associated with bacterial cell surface. Bacterial surface charge was neutralized by NPs, dependent on pH. The CuO NPs were more toxic than the ZnO NPs. The negative surface charge on colloids of extracellular polymeric substances (EPS) was reduced by Cu ions but not by CuO NPs; the EPS protected cells from CuO NPs-toxicity. CuO NPs-toxicity was eliminated by a Cu ion chelator, suggesting that ion release was involved. Neither NPs released alkaline phosphatase from the cells' periplasm, indicating minimal outer membrane damage. Accumulation of intracellular reactive oxygen species was correlated with CuO NPs lethality. Environmental deposition of NPs could create niches for ion release, with impacts on susceptible soil microbes.

Authors+Show Affiliations

Department of Biological Engineering, Utah State University, Logan, UT 84322, USA. cdimkpa@usu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21550151

Citation

Dimkpa, Christian O., et al. "Responses of a Soil Bacterium, Pseudomonas Chlororaphis O6 to Commercial Metal Oxide Nanoparticles Compared With Responses to Metal Ions." Environmental Pollution (Barking, Essex : 1987), vol. 159, no. 7, 2011, pp. 1749-56.
Dimkpa CO, Calder A, Britt DW, et al. Responses of a soil bacterium, Pseudomonas chlororaphis O6 to commercial metal oxide nanoparticles compared with responses to metal ions. Environ Pollut. 2011;159(7):1749-56.
Dimkpa, C. O., Calder, A., Britt, D. W., McLean, J. E., & Anderson, A. J. (2011). Responses of a soil bacterium, Pseudomonas chlororaphis O6 to commercial metal oxide nanoparticles compared with responses to metal ions. Environmental Pollution (Barking, Essex : 1987), 159(7), 1749-56. https://doi.org/10.1016/j.envpol.2011.04.020
Dimkpa CO, et al. Responses of a Soil Bacterium, Pseudomonas Chlororaphis O6 to Commercial Metal Oxide Nanoparticles Compared With Responses to Metal Ions. Environ Pollut. 2011;159(7):1749-56. PubMed PMID: 21550151.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Responses of a soil bacterium, Pseudomonas chlororaphis O6 to commercial metal oxide nanoparticles compared with responses to metal ions. AU - Dimkpa,Christian O, AU - Calder,Alyssa, AU - Britt,David W, AU - McLean,Joan E, AU - Anderson,Anne J, Y1 - 2011/05/06/ PY - 2010/07/26/received PY - 2011/04/11/revised PY - 2011/04/13/accepted PY - 2011/5/10/entrez PY - 2011/5/10/pubmed PY - 2011/8/31/medline SP - 1749 EP - 56 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ Pollut VL - 159 IS - 7 N2 - The toxicity of commercially-available CuO and ZnO nanoparticles (NPs) to pathogenic bacteria was compared for a beneficial rhizosphere isolate, Pseudomonas chlororaphis O6. The NPs aggregated, released ions to different extents under the conditions used for bacterial exposure, and associated with bacterial cell surface. Bacterial surface charge was neutralized by NPs, dependent on pH. The CuO NPs were more toxic than the ZnO NPs. The negative surface charge on colloids of extracellular polymeric substances (EPS) was reduced by Cu ions but not by CuO NPs; the EPS protected cells from CuO NPs-toxicity. CuO NPs-toxicity was eliminated by a Cu ion chelator, suggesting that ion release was involved. Neither NPs released alkaline phosphatase from the cells' periplasm, indicating minimal outer membrane damage. Accumulation of intracellular reactive oxygen species was correlated with CuO NPs lethality. Environmental deposition of NPs could create niches for ion release, with impacts on susceptible soil microbes. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/21550151/Responses_of_a_soil_bacterium_Pseudomonas_chlororaphis_O6_to_commercial_metal_oxide_nanoparticles_compared_with_responses_to_metal_ions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0269-7491(11)00228-4 DB - PRIME DP - Unbound Medicine ER -