Tags

Type your tag names separated by a space and hit enter

Stem cell differentiation to epidermal lineages on electrospun nanofibrous substrates for skin tissue engineering.
Acta Biomater. 2011 Aug; 7(8):3113-22.AB

Abstract

Bone marrow (BM) mesenchymal stem cells (MSC) capable of differentiating along the epidermal lineage on engineered nanofibrous scaffolds have great potential for bionanomaterial-cell transplantation therapy of skin wounds. MSC have been the focus of many tissue engineering studies, mainly because of their multipotential properties. We investigated the potential of human BM-derived MSC for epidermal cell differentiation in vitro on electrospun collagen/poly(l-lactic acid)-co-poly(3-caprolactone) (Coll/PLLCL) nanofibrous scaffolds. PLLCL and Coll/PLLCL nanofibrous scaffolds were fabricated by an electrospinning process and their chemical and mechanical characterization carried out by scanning electron microscopy (SEM), water contact angle determination, Fourier transform infrared spectroscopy, and tensile testing. The differentiation of MSC was carried out using epidermis inducing factors, including epidermal growth factor (EGF) and 1,25-dihydroxyvitamin D(3), in culture medium. The proliferation of MSC evaluated by cell proliferation assay showed that the number of cells grown on Coll/PLLCL nanofibrous scaffolds was significantly higher than those on PLLCL scaffolds. The SEM results showed that MSC differentiated on Coll/PLLCL nanofibrous scaffolds showed a round keratinocyte morphology and expressed keratin 10, filaggrin and partial involucrin protein by immunofluorescent microscopic studies. The interaction of MSC and nanofibers was studied and we concluded that the electrospun Coll/PLLCL nanofibers could mimic the native skin extracellular matrix environment and are promising substrates for advanced skin tissue engineering. Our studies on the differentiation of MSC along the epidermal lineage on nanofibrous scaffolds suggest their potential application in skin regeneration without regional differentiation.

Authors+Show Affiliations

Department of Mechanical Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21550425

Citation

Jin, Guorui, et al. "Stem Cell Differentiation to Epidermal Lineages On Electrospun Nanofibrous Substrates for Skin Tissue Engineering." Acta Biomaterialia, vol. 7, no. 8, 2011, pp. 3113-22.
Jin G, Prabhakaran MP, Ramakrishna S. Stem cell differentiation to epidermal lineages on electrospun nanofibrous substrates for skin tissue engineering. Acta Biomater. 2011;7(8):3113-22.
Jin, G., Prabhakaran, M. P., & Ramakrishna, S. (2011). Stem cell differentiation to epidermal lineages on electrospun nanofibrous substrates for skin tissue engineering. Acta Biomaterialia, 7(8), 3113-22. https://doi.org/10.1016/j.actbio.2011.04.017
Jin G, Prabhakaran MP, Ramakrishna S. Stem Cell Differentiation to Epidermal Lineages On Electrospun Nanofibrous Substrates for Skin Tissue Engineering. Acta Biomater. 2011;7(8):3113-22. PubMed PMID: 21550425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stem cell differentiation to epidermal lineages on electrospun nanofibrous substrates for skin tissue engineering. AU - Jin,Guorui, AU - Prabhakaran,Molamma P, AU - Ramakrishna,Seeram, Y1 - 2011/04/23/ PY - 2010/11/26/received PY - 2011/04/18/revised PY - 2011/04/19/accepted PY - 2011/5/10/entrez PY - 2011/5/10/pubmed PY - 2011/10/26/medline SP - 3113 EP - 22 JF - Acta biomaterialia JO - Acta Biomater VL - 7 IS - 8 N2 - Bone marrow (BM) mesenchymal stem cells (MSC) capable of differentiating along the epidermal lineage on engineered nanofibrous scaffolds have great potential for bionanomaterial-cell transplantation therapy of skin wounds. MSC have been the focus of many tissue engineering studies, mainly because of their multipotential properties. We investigated the potential of human BM-derived MSC for epidermal cell differentiation in vitro on electrospun collagen/poly(l-lactic acid)-co-poly(3-caprolactone) (Coll/PLLCL) nanofibrous scaffolds. PLLCL and Coll/PLLCL nanofibrous scaffolds were fabricated by an electrospinning process and their chemical and mechanical characterization carried out by scanning electron microscopy (SEM), water contact angle determination, Fourier transform infrared spectroscopy, and tensile testing. The differentiation of MSC was carried out using epidermis inducing factors, including epidermal growth factor (EGF) and 1,25-dihydroxyvitamin D(3), in culture medium. The proliferation of MSC evaluated by cell proliferation assay showed that the number of cells grown on Coll/PLLCL nanofibrous scaffolds was significantly higher than those on PLLCL scaffolds. The SEM results showed that MSC differentiated on Coll/PLLCL nanofibrous scaffolds showed a round keratinocyte morphology and expressed keratin 10, filaggrin and partial involucrin protein by immunofluorescent microscopic studies. The interaction of MSC and nanofibers was studied and we concluded that the electrospun Coll/PLLCL nanofibers could mimic the native skin extracellular matrix environment and are promising substrates for advanced skin tissue engineering. Our studies on the differentiation of MSC along the epidermal lineage on nanofibrous scaffolds suggest their potential application in skin regeneration without regional differentiation. SN - 1878-7568 UR - https://www.unboundmedicine.com/medline/citation/21550425/Stem_cell_differentiation_to_epidermal_lineages_on_electrospun_nanofibrous_substrates_for_skin_tissue_engineering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1742-7061(11)00182-6 DB - PRIME DP - Unbound Medicine ER -