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Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis.
Plant Cell Physiol. 2011 Jun; 52(6):1068-82.PC

Abstract

Debranching enzymes, which hydrolyze α-1 and 6-glucosidic linkages in α-polyglucans, play a dual role in the synthesis and degradation of starch in plants. A transposon-inserted rice mutant of isoamylase3 (isa3) contained an increased amount of starch in the leaf blade at the end of the night, indicating that ISA3 plays a role in the degradation of transitory starch during the night. An epitope-tagged ISA3 expressed in Escherichia coli exhibited hydrolytic activity on β-limit dextrin and amylopectin. We investigated whether ISA3 plays a role in amyloplast development and starch metabolism in the developing endosperm. ISA3-green fluorescent protein (GFP) fusion protein expressed under the control of the rice ISA3 promoter was targeted to the amyloplast stroma in the endosperm. Overexpression of ISA3 in the sugary1 mutant, which is deficient in ISA1 activity, did not convert water-soluble phytoglycogen to starch granules, indicating that ISA1 and ISA3 are not functionally redundant. Both overexpression and loss of function of ISA3 in the endosperm generated pleomorphic amyloplasts and starch granules. Furthermore, chloroplasts in the leaf blade of isa3 seedlings were large and pleomorphic. These results suggest that ISA3 facilitates starch metabolism and affects morphological characteristics of plastids in rice.

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Authors+Show Affiliations

Yun MS
Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai, Tsukuba, Japan.
Umemoto T
No affiliation info available
Kawagoe Y
No affiliation info available

MeSH

DNA Transposable ElementsEndospermEscherichia coliGene Expression Regulation, PlantGene Knockdown TechniquesGenotypeIsoamylaseMorphogenesisOryzaPlant LeavesPlant ProteinsPlants, Genetically ModifiedPlastidsRecombinant Fusion ProteinsStarchSubstrate Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21551159

Citation

Yun, Min-Soo, et al. "Rice Debranching Enzyme Isoamylase3 Facilitates Starch Metabolism and Affects Plastid Morphogenesis." Plant & Cell Physiology, vol. 52, no. 6, 2011, pp. 1068-82.
Yun MS, Umemoto T, Kawagoe Y. Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis. Plant Cell Physiol. 2011;52(6):1068-82.
Yun, M. S., Umemoto, T., & Kawagoe, Y. (2011). Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis. Plant & Cell Physiology, 52(6), 1068-82. https://doi.org/10.1093/pcp/pcr058
Yun MS, Umemoto T, Kawagoe Y. Rice Debranching Enzyme Isoamylase3 Facilitates Starch Metabolism and Affects Plastid Morphogenesis. Plant Cell Physiol. 2011;52(6):1068-82. PubMed PMID: 21551159.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis. AU - Yun,Min-Soo, AU - Umemoto,Takayuki, AU - Kawagoe,Yasushi, Y1 - 2011/05/06/ PY - 2011/5/10/entrez PY - 2011/5/10/pubmed PY - 2011/9/16/medline SP - 1068 EP - 82 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 52 IS - 6 N2 - Debranching enzymes, which hydrolyze α-1 and 6-glucosidic linkages in α-polyglucans, play a dual role in the synthesis and degradation of starch in plants. A transposon-inserted rice mutant of isoamylase3 (isa3) contained an increased amount of starch in the leaf blade at the end of the night, indicating that ISA3 plays a role in the degradation of transitory starch during the night. An epitope-tagged ISA3 expressed in Escherichia coli exhibited hydrolytic activity on β-limit dextrin and amylopectin. We investigated whether ISA3 plays a role in amyloplast development and starch metabolism in the developing endosperm. ISA3-green fluorescent protein (GFP) fusion protein expressed under the control of the rice ISA3 promoter was targeted to the amyloplast stroma in the endosperm. Overexpression of ISA3 in the sugary1 mutant, which is deficient in ISA1 activity, did not convert water-soluble phytoglycogen to starch granules, indicating that ISA1 and ISA3 are not functionally redundant. Both overexpression and loss of function of ISA3 in the endosperm generated pleomorphic amyloplasts and starch granules. Furthermore, chloroplasts in the leaf blade of isa3 seedlings were large and pleomorphic. These results suggest that ISA3 facilitates starch metabolism and affects morphological characteristics of plastids in rice. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/21551159/Rice_debranching_enzyme_isoamylase3_facilitates_starch_metabolism_and_affects_plastid_morphogenesis_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcr058 DB - PRIME DP - Unbound Medicine ER -