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Cardioprotection by ischemic postconditioning is lost in isolated perfused heart from diabetic rats: Involvement of transient receptor potential vanilloid 1, calcitonin gene-related peptide and substance P.
Regul Pept. 2011 Aug 08; 169(1-3):49-57.RP

Abstract

We previously found that the expression of transient receptor potential vanilloid 1 (TRPV1) and contents of calcitonin gene-related peptide (CGRP) and substance P (SP), two main neuropeptides released from TRPV1, were decreased in diabetic hearts. This study aimed to test whether decreased TRPV1, CGRP and SP levels were responsible for the loss of cardioprotection by ischemic postconditioning (IPostC) in isolated perfused heart from streptozotocin-induced diabetic rats. IPostC effectively protected non-diabetic hearts against ischemia/reperfusion injury by improving cardiac function and lowering creatine kinase (CK) and cardiac troponin I (cTnI) release, which could be abolished by inhibiting TRPV1, CGRP receptor or SP receptor. However, IPostC had no effect on cardiac function and the release of CK and cTnI in diabetic hearts regardless of whether TRPV1, CGRP receptor or SP receptor were inhibited. CGRP or SP-induced postconditioning significantly prevented both non-diabetic and diabetic hearts from ischemia/reperfusion injury by improving cardiac function and lowering CK and cTnI release. Additionally, IPostC markedly increased CGRP and SP release in non-diabetic hearts, which could be reversed with TRPV1 inhibition, but not CGRP receptor or SP receptor inhibition. However, IPostC failed to affect CGRP and SP release in diabetic hearts in the presence or absence of TRPV1, CGRP receptor or SP receptor inhibition. These results indicate that the loss of cardioprotection by IPostC during diabetes is partly associated with a failure to increase CGRP and SP release, likely due to decreased TRPV1 expression and CGRP and SP contents in diabetic hearts.

Authors+Show Affiliations

Department of Cardiology, Peking University People's Hospital, Beijing 100044, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21554904

Citation

Ren, Jing-Yi, et al. "Cardioprotection By Ischemic Postconditioning Is Lost in Isolated Perfused Heart From Diabetic Rats: Involvement of Transient Receptor Potential Vanilloid 1, Calcitonin Gene-related Peptide and Substance P." Regulatory Peptides, vol. 169, no. 1-3, 2011, pp. 49-57.
Ren JY, Song JX, Lu MY, et al. Cardioprotection by ischemic postconditioning is lost in isolated perfused heart from diabetic rats: Involvement of transient receptor potential vanilloid 1, calcitonin gene-related peptide and substance P. Regul Pept. 2011;169(1-3):49-57.
Ren, J. Y., Song, J. X., Lu, M. Y., & Chen, H. (2011). Cardioprotection by ischemic postconditioning is lost in isolated perfused heart from diabetic rats: Involvement of transient receptor potential vanilloid 1, calcitonin gene-related peptide and substance P. Regulatory Peptides, 169(1-3), 49-57. https://doi.org/10.1016/j.regpep.2011.04.004
Ren JY, et al. Cardioprotection By Ischemic Postconditioning Is Lost in Isolated Perfused Heart From Diabetic Rats: Involvement of Transient Receptor Potential Vanilloid 1, Calcitonin Gene-related Peptide and Substance P. Regul Pept. 2011 Aug 8;169(1-3):49-57. PubMed PMID: 21554904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cardioprotection by ischemic postconditioning is lost in isolated perfused heart from diabetic rats: Involvement of transient receptor potential vanilloid 1, calcitonin gene-related peptide and substance P. AU - Ren,Jing-Yi, AU - Song,Jun-Xian, AU - Lu,Ming-Yu, AU - Chen,Hong, Y1 - 2011/05/07/ PY - 2010/08/10/received PY - 2011/03/11/revised PY - 2011/04/16/accepted PY - 2011/5/11/entrez PY - 2011/5/11/pubmed PY - 2011/10/1/medline SP - 49 EP - 57 JF - Regulatory peptides JO - Regul Pept VL - 169 IS - 1-3 N2 - We previously found that the expression of transient receptor potential vanilloid 1 (TRPV1) and contents of calcitonin gene-related peptide (CGRP) and substance P (SP), two main neuropeptides released from TRPV1, were decreased in diabetic hearts. This study aimed to test whether decreased TRPV1, CGRP and SP levels were responsible for the loss of cardioprotection by ischemic postconditioning (IPostC) in isolated perfused heart from streptozotocin-induced diabetic rats. IPostC effectively protected non-diabetic hearts against ischemia/reperfusion injury by improving cardiac function and lowering creatine kinase (CK) and cardiac troponin I (cTnI) release, which could be abolished by inhibiting TRPV1, CGRP receptor or SP receptor. However, IPostC had no effect on cardiac function and the release of CK and cTnI in diabetic hearts regardless of whether TRPV1, CGRP receptor or SP receptor were inhibited. CGRP or SP-induced postconditioning significantly prevented both non-diabetic and diabetic hearts from ischemia/reperfusion injury by improving cardiac function and lowering CK and cTnI release. Additionally, IPostC markedly increased CGRP and SP release in non-diabetic hearts, which could be reversed with TRPV1 inhibition, but not CGRP receptor or SP receptor inhibition. However, IPostC failed to affect CGRP and SP release in diabetic hearts in the presence or absence of TRPV1, CGRP receptor or SP receptor inhibition. These results indicate that the loss of cardioprotection by IPostC during diabetes is partly associated with a failure to increase CGRP and SP release, likely due to decreased TRPV1 expression and CGRP and SP contents in diabetic hearts. SN - 1873-1686 UR - https://www.unboundmedicine.com/medline/citation/21554904/Cardioprotection_by_ischemic_postconditioning_is_lost_in_isolated_perfused_heart_from_diabetic_rats:_Involvement_of_transient_receptor_potential_vanilloid_1_calcitonin_gene_related_peptide_and_substance_P_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-0115(11)00067-X DB - PRIME DP - Unbound Medicine ER -