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A chiral HPLC-UV method for the quantification of dibenz[b,f]azepine-5-carboxamide derivatives in mouse plasma and brain tissue: eslicarbazepine acetate, carbamazepine and main metabolites.
J Sep Sci 2011; 34(12):1391-401JS

Abstract

For the first time, a selective and sensitive chiral HPLC-UV method was developed and fully validated for the simultaneous quantification of eslicarbazepine acetate (ESL), carbamazepine (CBZ), S-licarbazepine (S-Lic), R-licarbazepine (R-Lic), oxcarbazepine (OXC) and carbamazepine-10,11-epoxide (CBZ-E), in mouse plasma and brain homogenate supernatant. After the addition of chloramphenicol as the internal standard, samples were processed using an SPE procedure. The chiral chromatographic analysis was carried out on a LiChroCART 250-4 ChiraDex column, employing a mobile phase of water and methanol (88:12, v/v) pumped at 0.9 mL/min and the UV detector set at 235 nm. The assay was linear (r(2) ≥0.995) for ESL, CBZ, OXC, S-Lic, R-Lic and CBZ-E in the range of, respectively, 0.2-4, 0.4-30, 0.1-60, 0.2-60, 0.2-60 and 0.2-30 μg/mL, in plasma, and of 0.06-1.5 μg/mL for ESL, 0.12-15 μg/mL for CBZ and CBZ-E and 0.06-15 μg/mL for OXC and both licarbazepine (Lic) enantiomers in brain homogenate supernatant. The overall precision was within 8.71% and accuracy ranged from -7.55 to 8.97%. The recoveries of all the compounds were over 92.1%. Afterwards, the application of the method was demonstrated using real plasma and brain samples obtained from mice administered simultaneously with ESL and CBZ.

Authors+Show Affiliations

Pharmacology Department, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21557472

Citation

Fortuna, Ana, et al. "A Chiral HPLC-UV Method for the Quantification of Dibenz[b,f]azepine-5-carboxamide Derivatives in Mouse Plasma and Brain Tissue: Eslicarbazepine Acetate, Carbamazepine and Main Metabolites." Journal of Separation Science, vol. 34, no. 12, 2011, pp. 1391-401.
Fortuna A, Bicker J, Alves G, et al. A chiral HPLC-UV method for the quantification of dibenz[b,f]azepine-5-carboxamide derivatives in mouse plasma and brain tissue: eslicarbazepine acetate, carbamazepine and main metabolites. J Sep Sci. 2011;34(12):1391-401.
Fortuna, A., Bicker, J., Alves, G., Falcão, A., & Soares-da-Silva, P. (2011). A chiral HPLC-UV method for the quantification of dibenz[b,f]azepine-5-carboxamide derivatives in mouse plasma and brain tissue: eslicarbazepine acetate, carbamazepine and main metabolites. Journal of Separation Science, 34(12), pp. 1391-401. doi:10.1002/jssc.201100099.
Fortuna A, et al. A Chiral HPLC-UV Method for the Quantification of Dibenz[b,f]azepine-5-carboxamide Derivatives in Mouse Plasma and Brain Tissue: Eslicarbazepine Acetate, Carbamazepine and Main Metabolites. J Sep Sci. 2011;34(12):1391-401. PubMed PMID: 21557472.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A chiral HPLC-UV method for the quantification of dibenz[b,f]azepine-5-carboxamide derivatives in mouse plasma and brain tissue: eslicarbazepine acetate, carbamazepine and main metabolites. AU - Fortuna,Ana, AU - Bicker,Joana, AU - Alves,Gilberto, AU - Falcão,Amílcar, AU - Soares-da-Silva,Patrício, Y1 - 2011/05/09/ PY - 2011/02/06/received PY - 2011/03/19/revised PY - 2011/03/21/accepted PY - 2011/5/11/entrez PY - 2011/5/11/pubmed PY - 2011/9/29/medline SP - 1391 EP - 401 JF - Journal of separation science JO - J Sep Sci VL - 34 IS - 12 N2 - For the first time, a selective and sensitive chiral HPLC-UV method was developed and fully validated for the simultaneous quantification of eslicarbazepine acetate (ESL), carbamazepine (CBZ), S-licarbazepine (S-Lic), R-licarbazepine (R-Lic), oxcarbazepine (OXC) and carbamazepine-10,11-epoxide (CBZ-E), in mouse plasma and brain homogenate supernatant. After the addition of chloramphenicol as the internal standard, samples were processed using an SPE procedure. The chiral chromatographic analysis was carried out on a LiChroCART 250-4 ChiraDex column, employing a mobile phase of water and methanol (88:12, v/v) pumped at 0.9 mL/min and the UV detector set at 235 nm. The assay was linear (r(2) ≥0.995) for ESL, CBZ, OXC, S-Lic, R-Lic and CBZ-E in the range of, respectively, 0.2-4, 0.4-30, 0.1-60, 0.2-60, 0.2-60 and 0.2-30 μg/mL, in plasma, and of 0.06-1.5 μg/mL for ESL, 0.12-15 μg/mL for CBZ and CBZ-E and 0.06-15 μg/mL for OXC and both licarbazepine (Lic) enantiomers in brain homogenate supernatant. The overall precision was within 8.71% and accuracy ranged from -7.55 to 8.97%. The recoveries of all the compounds were over 92.1%. Afterwards, the application of the method was demonstrated using real plasma and brain samples obtained from mice administered simultaneously with ESL and CBZ. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/21557472/A_chiral_HPLC_UV_method_for_the_quantification_of_dibenz[bf]azepine_5_carboxamide_derivatives_in_mouse_plasma_and_brain_tissue:_eslicarbazepine_acetate_carbamazepine_and_main_metabolites_ L2 - https://doi.org/10.1002/jssc.201100099 DB - PRIME DP - Unbound Medicine ER -