Tags

Type your tag names separated by a space and hit enter

Antioxidant activity of uruguayan propolis. In vitro and cellular assays.
J Agric Food Chem. 2011 Jun 22; 59(12):6430-7.JA

Abstract

The antioxidant capacity of propolis from the southern region of Uruguay was evaluated using in vitro as well as cellular assays. Free radical scavenging capacity was assessed by ORAC, obtaining values significantly higher than those of other natural products (8000 μmol Trolox equiv/g propolis). ORAC values correlated well with total polyphenol content (determined by Folin-Ciocalteu method) and UV absorption. Total polyphenol content (150 mg gallic acid equiv/g propolis) and flavonoids (45 mg quercetin equiv/g propolis) were similar to values reported for southern Brazilian (group 3) and Argentinean propolis. Flavonoid composition determined by RP-HPLC indicates a strong poplar-tree origin. Samples high in polyphenols efficiently inhibit low-density lipoprotein lipoperoxidation and tyrosine nitration. In addition, Uruguayan propolis was found to induce the expression of endothelial nitric oxide synthase and inhibit endothelial NADPH oxidase, suggesting a potential cardiovascular benefit by increasing nitric oxide bioavailability in the endothelium.

Authors+Show Affiliations

Laboratorio de Fisicoquímica Biológica y Enzimología, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República (UdelaR), Iguá 4225, 11400 Montevideo, Uruguay.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21563839

Citation

Silva, Verónica, et al. "Antioxidant Activity of Uruguayan Propolis. in Vitro and Cellular Assays." Journal of Agricultural and Food Chemistry, vol. 59, no. 12, 2011, pp. 6430-7.
Silva V, Genta G, Möller MN, et al. Antioxidant activity of uruguayan propolis. In vitro and cellular assays. J Agric Food Chem. 2011;59(12):6430-7.
Silva, V., Genta, G., Möller, M. N., Masner, M., Thomson, L., Romero, N., Radi, R., Fernandes, D. C., Laurindo, F. R., Heinzen, H., Fierro, W., & Denicola, A. (2011). Antioxidant activity of uruguayan propolis. In vitro and cellular assays. Journal of Agricultural and Food Chemistry, 59(12), 6430-7. https://doi.org/10.1021/jf201032y
Silva V, et al. Antioxidant Activity of Uruguayan Propolis. in Vitro and Cellular Assays. J Agric Food Chem. 2011 Jun 22;59(12):6430-7. PubMed PMID: 21563839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity of uruguayan propolis. In vitro and cellular assays. AU - Silva,Verónica, AU - Genta,Gonzalo, AU - Möller,Matías N, AU - Masner,Martín, AU - Thomson,Leonor, AU - Romero,Natalia, AU - Radi,Rafael, AU - Fernandes,Denise C, AU - Laurindo,Francisco R M, AU - Heinzen,Horacio, AU - Fierro,Walter, AU - Denicola,Ana, Y1 - 2011/05/26/ PY - 2011/5/14/entrez PY - 2011/5/14/pubmed PY - 2011/10/13/medline SP - 6430 EP - 7 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 59 IS - 12 N2 - The antioxidant capacity of propolis from the southern region of Uruguay was evaluated using in vitro as well as cellular assays. Free radical scavenging capacity was assessed by ORAC, obtaining values significantly higher than those of other natural products (8000 μmol Trolox equiv/g propolis). ORAC values correlated well with total polyphenol content (determined by Folin-Ciocalteu method) and UV absorption. Total polyphenol content (150 mg gallic acid equiv/g propolis) and flavonoids (45 mg quercetin equiv/g propolis) were similar to values reported for southern Brazilian (group 3) and Argentinean propolis. Flavonoid composition determined by RP-HPLC indicates a strong poplar-tree origin. Samples high in polyphenols efficiently inhibit low-density lipoprotein lipoperoxidation and tyrosine nitration. In addition, Uruguayan propolis was found to induce the expression of endothelial nitric oxide synthase and inhibit endothelial NADPH oxidase, suggesting a potential cardiovascular benefit by increasing nitric oxide bioavailability in the endothelium. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/21563839/Antioxidant_activity_of_uruguayan_propolis__In_vitro_and_cellular_assays_ L2 - https://dx.doi.org/10.1021/jf201032y DB - PRIME DP - Unbound Medicine ER -