Tags

Type your tag names separated by a space and hit enter

Evaluation of free-radical quenching properties of standard Ayurvedic formulation Vayasthapana Rasayana.
BMC Complement Altern Med. 2011 May 12; 11:38.BC

Abstract

BACKGROUND

Cellular damage induced by free-radicals like Reactive Oxygen and Nitrogen Species (ROS and RNS) has been implicated in several disorders and diseases, including ageing. Hence naturally occurring anti-oxidant rich-herbs play a vital role in combating these conditions. The present study was carried out to investigate the in vitro free-radical quenching capacity of a known Ayurvedic poly-herbal formulation called Vayasthapana Rasayana.

METHODS

Methanol extracts of Vayasthapana Rasayana formulation (VRF) were studied for in vitro total antioxidant activity along with phenolic content and reducing power. In vitro assays like DPPH, FRAP, ABTS scavenging to evaluate radical quenching potential were performed.

RESULTS

The formulation has shown 94% at 0.1 mg/ml DPPH free-radical scavenging activity as against 84% at 0.1 mg/ml for standard ascorbic acid (IC₅₀ value 5.51 μg/ml for VRF and 39 μg/ml for standard). It has a significant higher ferric reducing potential also (OD 0.87 at 700 nm & 0.21 at 0.1 mg/ml for VRF and standard, respectively). The total phenolic content (gallic acid equivalent) of the VRF is 8.3 mg per g of dry mass. Total antioxidant capacity of the formulation, estimated by FRAP was 1150 ± 5 μM Fe(II)/g dry mass. ABTS radical scavenging activity of VRF was 69.55 ± 0.21% at 100 μg/ml concentration with a IC50 value of 69.87 μg/ml as against 9% and 95% by ascorbic acid and Trolox (at 70.452 μg/ml and 0.250 μg/ml concentrations, respectively).

CONCLUSION

In Indian traditional Ayurvedic system, use of VRF is in regular practice for mainly combating age-related disorders and diseases as many of the components of the Rasayana are known for their free-radical scavenging activity. This study has validated the potential use of VRF as an anti-oxidant to fight age-related problems.

Authors+Show Affiliations

Rajiv Gandhi Institute for IT and Biotechnology, Bharati Vidyapeeth University, Pune-Satara Road, Pune-411 046, Maharashtra, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21569386

Citation

Mukherjee, Sourav, et al. "Evaluation of Free-radical Quenching Properties of Standard Ayurvedic Formulation Vayasthapana Rasayana." BMC Complementary and Alternative Medicine, vol. 11, 2011, p. 38.
Mukherjee S, Pawar N, Kulkarni O, et al. Evaluation of free-radical quenching properties of standard Ayurvedic formulation Vayasthapana Rasayana. BMC Complement Altern Med. 2011;11:38.
Mukherjee, S., Pawar, N., Kulkarni, O., Nagarkar, B., Thopte, S., Bhujbal, A., & Pawar, P. (2011). Evaluation of free-radical quenching properties of standard Ayurvedic formulation Vayasthapana Rasayana. BMC Complementary and Alternative Medicine, 11, 38. https://doi.org/10.1186/1472-6882-11-38
Mukherjee S, et al. Evaluation of Free-radical Quenching Properties of Standard Ayurvedic Formulation Vayasthapana Rasayana. BMC Complement Altern Med. 2011 May 12;11:38. PubMed PMID: 21569386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of free-radical quenching properties of standard Ayurvedic formulation Vayasthapana Rasayana. AU - Mukherjee,Sourav, AU - Pawar,Nayana, AU - Kulkarni,Omkar, AU - Nagarkar,Bhagyashri, AU - Thopte,Shrikant, AU - Bhujbal,Akshay, AU - Pawar,Pankaj, Y1 - 2011/05/12/ PY - 2010/07/09/received PY - 2011/05/12/accepted PY - 2011/5/17/entrez PY - 2011/5/17/pubmed PY - 2011/10/1/medline SP - 38 EP - 38 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 11 N2 - BACKGROUND: Cellular damage induced by free-radicals like Reactive Oxygen and Nitrogen Species (ROS and RNS) has been implicated in several disorders and diseases, including ageing. Hence naturally occurring anti-oxidant rich-herbs play a vital role in combating these conditions. The present study was carried out to investigate the in vitro free-radical quenching capacity of a known Ayurvedic poly-herbal formulation called Vayasthapana Rasayana. METHODS: Methanol extracts of Vayasthapana Rasayana formulation (VRF) were studied for in vitro total antioxidant activity along with phenolic content and reducing power. In vitro assays like DPPH, FRAP, ABTS scavenging to evaluate radical quenching potential were performed. RESULTS: The formulation has shown 94% at 0.1 mg/ml DPPH free-radical scavenging activity as against 84% at 0.1 mg/ml for standard ascorbic acid (IC₅₀ value 5.51 μg/ml for VRF and 39 μg/ml for standard). It has a significant higher ferric reducing potential also (OD 0.87 at 700 nm & 0.21 at 0.1 mg/ml for VRF and standard, respectively). The total phenolic content (gallic acid equivalent) of the VRF is 8.3 mg per g of dry mass. Total antioxidant capacity of the formulation, estimated by FRAP was 1150 ± 5 μM Fe(II)/g dry mass. ABTS radical scavenging activity of VRF was 69.55 ± 0.21% at 100 μg/ml concentration with a IC50 value of 69.87 μg/ml as against 9% and 95% by ascorbic acid and Trolox (at 70.452 μg/ml and 0.250 μg/ml concentrations, respectively). CONCLUSION: In Indian traditional Ayurvedic system, use of VRF is in regular practice for mainly combating age-related disorders and diseases as many of the components of the Rasayana are known for their free-radical scavenging activity. This study has validated the potential use of VRF as an anti-oxidant to fight age-related problems. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/21569386/Evaluation_of_free_radical_quenching_properties_of_standard_Ayurvedic_formulation_Vayasthapana_Rasayana_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-11-38 DB - PRIME DP - Unbound Medicine ER -