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In vitro studies of Gynura divaricata (L.) DC extracts as inhibitors of key enzymes relevant for type 2 diabetes and hypertension.
J Ethnopharmacol. 2011 Jun 22; 136(2):305-8.JE

Abstract

AIM OF THE STUDY

To evaluate traditionally used herb, Gynura divaricata (L.) DC (Bai Bei San Qi) as in vitro inhibitors of key enzymes involved in the pathogenesis of hyperglycemia and hypertension. We also determined the distribution of enzyme inhibitory activities in different aqueous and non-aqueous extracts.

MATERIALS AND METHODS

The water extract (extract 1) from the aerial parts of Gynura divaricata (L.) was prepared first and then partitioned sequentially with n-butanol, ethyl acetate, and macroporous adsorptive resin (HPD-40) to yield extracts 2-4; the remaining water phase was named extract 5. Angiotensin-1 converting enzyme (ACE), α-amylase α-glycosidase inhibitory activities of the extracts were determined in vitro and chemical composition including total sugar, protein, flavonoid and total alkaloids in the extract were also evaluated.

RESULTS

The water extract of this herb significantly inhibited (p<0.05) ACE activity (IC(50)=0.37 mg/ml) and showed a moderate potential hypoglycemic effect via in vitro α-amylase (IC(50)=1.36 mg/ml) and α-glycosidase (IC(50)=2.17 mg/ml) inhibition in dose-dependent manner. Further partitioning of the water extract (extracts 2-4) resulted in higher α-amylase inhibitory activities in extract 2 and 3. For α-glycosidase inhibition, extract 3 gave the highest inhibition. ACE inhibitory activities of the extracts were not improved by partitioning. Sugar, protein, flavonoid and alkaloid were found in water extract but only a small portion was partitioned in the n-butanol extract. However, a large portion of the flavonoids and alkaloids were found in ethyl acetate extract.

CONCLUSION

The results confirmed potential empirical use of Gynura divaricata (L.) DC for the management of hyperglycemia as well as hypertension. The active compounds for inhibition of α-amylase and α-glycosidase inhibition were flavonoids and alkaloids while ACE inhibition probably resulted from synergic effects of all the herb compounds.

Authors+Show Affiliations

Chemistry Institute of Pharmaceutical Resources, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21570455

Citation

Wu, Tingting, et al. "In Vitro Studies of Gynura Divaricata (L.) DC Extracts as Inhibitors of Key Enzymes Relevant for Type 2 Diabetes and Hypertension." Journal of Ethnopharmacology, vol. 136, no. 2, 2011, pp. 305-8.
Wu T, Zhou X, Deng Y, et al. In vitro studies of Gynura divaricata (L.) DC extracts as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. J Ethnopharmacol. 2011;136(2):305-8.
Wu, T., Zhou, X., Deng, Y., Jing, Q., Li, M., & Yuan, L. (2011). In vitro studies of Gynura divaricata (L.) DC extracts as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Journal of Ethnopharmacology, 136(2), 305-8. https://doi.org/10.1016/j.jep.2011.04.059
Wu T, et al. In Vitro Studies of Gynura Divaricata (L.) DC Extracts as Inhibitors of Key Enzymes Relevant for Type 2 Diabetes and Hypertension. J Ethnopharmacol. 2011 Jun 22;136(2):305-8. PubMed PMID: 21570455.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro studies of Gynura divaricata (L.) DC extracts as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. AU - Wu,Tingting, AU - Zhou,Xueting, AU - Deng,Yafei, AU - Jing,Qing, AU - Li,Min, AU - Yuan,Lujaing, Y1 - 2011/05/06/ PY - 2010/09/20/received PY - 2011/04/12/revised PY - 2011/04/20/accepted PY - 2011/5/17/entrez PY - 2011/5/17/pubmed PY - 2011/12/15/medline SP - 305 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 136 IS - 2 N2 - AIM OF THE STUDY: To evaluate traditionally used herb, Gynura divaricata (L.) DC (Bai Bei San Qi) as in vitro inhibitors of key enzymes involved in the pathogenesis of hyperglycemia and hypertension. We also determined the distribution of enzyme inhibitory activities in different aqueous and non-aqueous extracts. MATERIALS AND METHODS: The water extract (extract 1) from the aerial parts of Gynura divaricata (L.) was prepared first and then partitioned sequentially with n-butanol, ethyl acetate, and macroporous adsorptive resin (HPD-40) to yield extracts 2-4; the remaining water phase was named extract 5. Angiotensin-1 converting enzyme (ACE), α-amylase α-glycosidase inhibitory activities of the extracts were determined in vitro and chemical composition including total sugar, protein, flavonoid and total alkaloids in the extract were also evaluated. RESULTS: The water extract of this herb significantly inhibited (p<0.05) ACE activity (IC(50)=0.37 mg/ml) and showed a moderate potential hypoglycemic effect via in vitro α-amylase (IC(50)=1.36 mg/ml) and α-glycosidase (IC(50)=2.17 mg/ml) inhibition in dose-dependent manner. Further partitioning of the water extract (extracts 2-4) resulted in higher α-amylase inhibitory activities in extract 2 and 3. For α-glycosidase inhibition, extract 3 gave the highest inhibition. ACE inhibitory activities of the extracts were not improved by partitioning. Sugar, protein, flavonoid and alkaloid were found in water extract but only a small portion was partitioned in the n-butanol extract. However, a large portion of the flavonoids and alkaloids were found in ethyl acetate extract. CONCLUSION: The results confirmed potential empirical use of Gynura divaricata (L.) DC for the management of hyperglycemia as well as hypertension. The active compounds for inhibition of α-amylase and α-glycosidase inhibition were flavonoids and alkaloids while ACE inhibition probably resulted from synergic effects of all the herb compounds. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21570455/In_vitro_studies_of_Gynura_divaricata__L___DC_extracts_as_inhibitors_of_key_enzymes_relevant_for_type_2_diabetes_and_hypertension_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00311-4 DB - PRIME DP - Unbound Medicine ER -