Tags

Type your tag names separated by a space and hit enter

In vitro cytotoxicity and phototoxicity study of cosmetics colorants.
Toxicol In Vitro 2011; 25(6):1242-50TV

Abstract

The aim of the work was early identification of preventable risk factors connected with the consumers usage of products of everyday use, such as cosmetics, toys and children products, and other materials intended for contact with human skin. The risk factor is represented by substances with irritation potential and subsequent possible sensitisation, resulting in negative impact on human physical and psychical health with social and societal consequences. The legislation for cosmetics, chemical substances and other products requires for hazard identification the application of alternative toxicological methods in vitro without the use of animals. For this reason we used a battery of alternative assays in vitro, based on cell cultures. Progressive methods of molecular biology, based on fluorimetry and fluorescence, were employed for identification of early morphological and functional changes on cellular level. Four colorants frequently used in cosmetics (P-WS Caramel, Chlorophyllin, Unicert Red K 7054-J and Unicert Red K 7008-J) were tested on cell line NIH3T3 (mouse fibroblast cell) and 3T3 Balb/c with/without UV irradiation (dose 5 J cm(-2)). Fluorescence methods for the study of cell damage using fluorescence probes offer results for the evaluation of cytotoxicity and cell viability of adherent cells. We detected intracellular production of ROS investigated by molecular probe CM-H(2)DCFDA, which is primarily sensitive to the increased production of hydrogen peroxide or its downstream products. Toxic effects on the cellular level were identified by viability tests using Neutral Red uptake and MTT assay, where the live cells reduce yellow soluble 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to insoluble formazan crystals. The reaction was investigated on mitochondrial membrane of living cells and the type of cell death was determined using Apoptosis detection kit. Cytotoxicity tests revealed health risks of using Chlorophyllin and Unicert Red K 7054-J.

Authors+Show Affiliations

Department of Medical Biophysics, Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic. katerina.tomankova@upol.czNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21570462

Citation

Tomankova, K, et al. "In Vitro Cytotoxicity and Phototoxicity Study of Cosmetics Colorants." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 25, no. 6, 2011, pp. 1242-50.
Tomankova K, Kejlova K, Binder S, et al. In vitro cytotoxicity and phototoxicity study of cosmetics colorants. Toxicol In Vitro. 2011;25(6):1242-50.
Tomankova, K., Kejlova, K., Binder, S., Daskova, A., Zapletalova, J., Bendova, H., ... Jirova, D. (2011). In vitro cytotoxicity and phototoxicity study of cosmetics colorants. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 25(6), pp. 1242-50. doi:10.1016/j.tiv.2011.04.026.
Tomankova K, et al. In Vitro Cytotoxicity and Phototoxicity Study of Cosmetics Colorants. Toxicol In Vitro. 2011;25(6):1242-50. PubMed PMID: 21570462.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro cytotoxicity and phototoxicity study of cosmetics colorants. AU - Tomankova,K, AU - Kejlova,K, AU - Binder,S, AU - Daskova,A, AU - Zapletalova,J, AU - Bendova,H, AU - Kolarova,H, AU - Jirova,D, Y1 - 2011/05/04/ PY - 2010/08/31/received PY - 2011/04/19/revised PY - 2011/04/27/accepted PY - 2011/5/17/entrez PY - 2011/5/17/pubmed PY - 2011/12/13/medline SP - 1242 EP - 50 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 25 IS - 6 N2 - The aim of the work was early identification of preventable risk factors connected with the consumers usage of products of everyday use, such as cosmetics, toys and children products, and other materials intended for contact with human skin. The risk factor is represented by substances with irritation potential and subsequent possible sensitisation, resulting in negative impact on human physical and psychical health with social and societal consequences. The legislation for cosmetics, chemical substances and other products requires for hazard identification the application of alternative toxicological methods in vitro without the use of animals. For this reason we used a battery of alternative assays in vitro, based on cell cultures. Progressive methods of molecular biology, based on fluorimetry and fluorescence, were employed for identification of early morphological and functional changes on cellular level. Four colorants frequently used in cosmetics (P-WS Caramel, Chlorophyllin, Unicert Red K 7054-J and Unicert Red K 7008-J) were tested on cell line NIH3T3 (mouse fibroblast cell) and 3T3 Balb/c with/without UV irradiation (dose 5 J cm(-2)). Fluorescence methods for the study of cell damage using fluorescence probes offer results for the evaluation of cytotoxicity and cell viability of adherent cells. We detected intracellular production of ROS investigated by molecular probe CM-H(2)DCFDA, which is primarily sensitive to the increased production of hydrogen peroxide or its downstream products. Toxic effects on the cellular level were identified by viability tests using Neutral Red uptake and MTT assay, where the live cells reduce yellow soluble 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to insoluble formazan crystals. The reaction was investigated on mitochondrial membrane of living cells and the type of cell death was determined using Apoptosis detection kit. Cytotoxicity tests revealed health risks of using Chlorophyllin and Unicert Red K 7054-J. SN - 1879-3177 UR - https://www.unboundmedicine.com/medline/citation/21570462/In_vitro_cytotoxicity_and_phototoxicity_study_of_cosmetics_colorants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(11)00122-6 DB - PRIME DP - Unbound Medicine ER -