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A preliminary investigation on the distribution of cannabinoids in man.
Forensic Sci Int 2011; 210(1-3):e7-e11FS

Abstract

An LC/MS/MS procedure to determine THC along with its major metabolites 11-OH-THC, THC-COOH and its glucuronide as well as the cannabinoids CBD and CBN was applied to 5 post mortem cases to study their distribution into some less commonly studied matrices. Analytes were determined in fluids and tissue homogenates following protein precipitation and liquid-liquid extraction. Gall bladder fluid exhibited maximum concentrations of all analytes except THC, which was detectable in high concentrations in muscle tissue along with CBD. THC was also present in lung specimens, whereas its concentration in liver samples was low or not detectable at all. Liver und kidney specimens contained appreciable amounts of THC-COOglu. Findings from bile support extensive enterohepatic recirculation of the glucuronide. Muscle tissue seems an interesting specimen to detect multiple cannabis use, and brain may serve as an alternative specimen for blood; nevertheless, the present findings should be substantiated by further investigations.

Authors+Show Affiliations

Institute of Legal Medicine and Traffic Medicine, University Hospital, Voss-Str. 2, 69115 Heidelberg, Germany.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21570784

Citation

Gronewold, Antonia, and Gisela Skopp. "A Preliminary Investigation On the Distribution of Cannabinoids in Man." Forensic Science International, vol. 210, no. 1-3, 2011, pp. e7-e11.
Gronewold A, Skopp G. A preliminary investigation on the distribution of cannabinoids in man. Forensic Sci Int. 2011;210(1-3):e7-e11.
Gronewold, A., & Skopp, G. (2011). A preliminary investigation on the distribution of cannabinoids in man. Forensic Science International, 210(1-3), pp. e7-e11. doi:10.1016/j.forsciint.2011.04.010.
Gronewold A, Skopp G. A Preliminary Investigation On the Distribution of Cannabinoids in Man. Forensic Sci Int. 2011 Jul 15;210(1-3):e7-e11. PubMed PMID: 21570784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A preliminary investigation on the distribution of cannabinoids in man. AU - Gronewold,Antonia, AU - Skopp,Gisela, Y1 - 2011/05/13/ PY - 2010/09/14/received PY - 2011/04/04/revised PY - 2011/04/09/accepted PY - 2011/5/17/entrez PY - 2011/5/17/pubmed PY - 2011/11/4/medline SP - e7 EP - e11 JF - Forensic science international JO - Forensic Sci. Int. VL - 210 IS - 1-3 N2 - An LC/MS/MS procedure to determine THC along with its major metabolites 11-OH-THC, THC-COOH and its glucuronide as well as the cannabinoids CBD and CBN was applied to 5 post mortem cases to study their distribution into some less commonly studied matrices. Analytes were determined in fluids and tissue homogenates following protein precipitation and liquid-liquid extraction. Gall bladder fluid exhibited maximum concentrations of all analytes except THC, which was detectable in high concentrations in muscle tissue along with CBD. THC was also present in lung specimens, whereas its concentration in liver samples was low or not detectable at all. Liver und kidney specimens contained appreciable amounts of THC-COOglu. Findings from bile support extensive enterohepatic recirculation of the glucuronide. Muscle tissue seems an interesting specimen to detect multiple cannabis use, and brain may serve as an alternative specimen for blood; nevertheless, the present findings should be substantiated by further investigations. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/21570784/abstract/A_preliminary_investigation_on_the_distribution_of_cannabinoids_in_man_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(11)00192-7 DB - PRIME DP - Unbound Medicine ER -