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Patients suffering from non-IgE-mediated cow's milk protein intolerance cannot be diagnosed based on IgG subclass or IgA responses to milk allergens.
Allergy. 2011 Sep; 66(9):1201-7.A

Abstract

BACKGROUND

Cow's milk is one of the most common causes of food allergy. In two-thirds of patients, adverse symptoms following milk ingestion are caused by IgE-mediated allergic reactions, whereas for one-third, the mechanisms are unknown. Aim of this study was to investigate whether patients suffering from non-IgE-mediated cow's milk protein intolerance can be distinguished from persons without cow's milk protein intolerance based on serological measurement of IgG and IgA specific for purified cow's milk antigens.

METHODS

We determined IgG(1-4) subclass and IgA antibody levels to purified recombinant αS1-casein, αS2-casein, β-casein, κ-casein, α-lactalbumin, and β-lactoglobulin in four patient groups by ELISA: Patients with IgE-mediated cow's milk allergy (CMA, n=25), patients with non-IgE-mediated cow's milk protein intolerance (CMPI, n=19), patients with gastrointestinal symptoms not associated with cow's milk ingestion (GI, n=15) and control persons without gastrointestinal problems (C, n=26). Cow's milk-specific IgE levels were determined by ImmunoCAP.

RESULTS

Only CMA patients had IgE antibodies to cow's milk. Cow's milk allergic patients mounted the highest IgG(1) and IgG(4) antibody levels to αS1-casein, αS2-casein, β-casein, κ-casein, and α-lactalbumin. No elevated levels of IgG(4) , IgA, and complement-binding IgG subclasses (IgG(1) , IgG(2) , IgG(3)) to purified cow's milk allergens were found within the CMPI patients compared to persons without cow's milk protein intolerance (GI and C groups).

CONCLUSION

Cow's milk protein intolerant patients cannot be distinguished from persons without cow's milk protein intolerance on the basis of IgG subclass or IgA reactivity to cow's milk allergens.

Authors+Show Affiliations

Department of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21575008

Citation

Hochwallner, H, et al. "Patients Suffering From non-IgE-mediated Cow's Milk Protein Intolerance Cannot Be Diagnosed Based On IgG Subclass or IgA Responses to Milk Allergens." Allergy, vol. 66, no. 9, 2011, pp. 1201-7.
Hochwallner H, Schulmeister U, Swoboda I, et al. Patients suffering from non-IgE-mediated cow's milk protein intolerance cannot be diagnosed based on IgG subclass or IgA responses to milk allergens. Allergy. 2011;66(9):1201-7.
Hochwallner, H., Schulmeister, U., Swoboda, I., Twaroch, T. E., Vogelsang, H., Kazemi-Shirazi, L., Kundi, M., Balic, N., Quirce, S., Rumpold, H., Fröschl, R., Horak, F., Tichatschek, B., Stefanescu, C. L., Szépfalusi, Z., Papadopoulos, N. G., Mari, A., Ebner, C., Pauli, G., ... Spitzauer, S. (2011). Patients suffering from non-IgE-mediated cow's milk protein intolerance cannot be diagnosed based on IgG subclass or IgA responses to milk allergens. Allergy, 66(9), 1201-7. https://doi.org/10.1111/j.1398-9995.2011.02635.x
Hochwallner H, et al. Patients Suffering From non-IgE-mediated Cow's Milk Protein Intolerance Cannot Be Diagnosed Based On IgG Subclass or IgA Responses to Milk Allergens. Allergy. 2011;66(9):1201-7. PubMed PMID: 21575008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Patients suffering from non-IgE-mediated cow's milk protein intolerance cannot be diagnosed based on IgG subclass or IgA responses to milk allergens. AU - Hochwallner,H, AU - Schulmeister,U, AU - Swoboda,I, AU - Twaroch,T E, AU - Vogelsang,H, AU - Kazemi-Shirazi,L, AU - Kundi,M, AU - Balic,N, AU - Quirce,S, AU - Rumpold,H, AU - Fröschl,R, AU - Horak,F, AU - Tichatschek,B, AU - Stefanescu,C L, AU - Szépfalusi,Z, AU - Papadopoulos,N G, AU - Mari,A, AU - Ebner,C, AU - Pauli,G, AU - Valenta,R, AU - Spitzauer,S, Y1 - 2011/05/17/ PY - 2011/5/18/entrez PY - 2011/5/18/pubmed PY - 2011/12/13/medline SP - 1201 EP - 7 JF - Allergy JO - Allergy VL - 66 IS - 9 N2 - BACKGROUND: Cow's milk is one of the most common causes of food allergy. In two-thirds of patients, adverse symptoms following milk ingestion are caused by IgE-mediated allergic reactions, whereas for one-third, the mechanisms are unknown. Aim of this study was to investigate whether patients suffering from non-IgE-mediated cow's milk protein intolerance can be distinguished from persons without cow's milk protein intolerance based on serological measurement of IgG and IgA specific for purified cow's milk antigens. METHODS: We determined IgG(1-4) subclass and IgA antibody levels to purified recombinant αS1-casein, αS2-casein, β-casein, κ-casein, α-lactalbumin, and β-lactoglobulin in four patient groups by ELISA: Patients with IgE-mediated cow's milk allergy (CMA, n=25), patients with non-IgE-mediated cow's milk protein intolerance (CMPI, n=19), patients with gastrointestinal symptoms not associated with cow's milk ingestion (GI, n=15) and control persons without gastrointestinal problems (C, n=26). Cow's milk-specific IgE levels were determined by ImmunoCAP. RESULTS: Only CMA patients had IgE antibodies to cow's milk. Cow's milk allergic patients mounted the highest IgG(1) and IgG(4) antibody levels to αS1-casein, αS2-casein, β-casein, κ-casein, and α-lactalbumin. No elevated levels of IgG(4) , IgA, and complement-binding IgG subclasses (IgG(1) , IgG(2) , IgG(3)) to purified cow's milk allergens were found within the CMPI patients compared to persons without cow's milk protein intolerance (GI and C groups). CONCLUSION: Cow's milk protein intolerant patients cannot be distinguished from persons without cow's milk protein intolerance on the basis of IgG subclass or IgA reactivity to cow's milk allergens. SN - 1398-9995 UR - https://www.unboundmedicine.com/medline/citation/21575008/Patients_suffering_from_non_IgE_mediated_cow's_milk_protein_intolerance_cannot_be_diagnosed_based_on_IgG_subclass_or_IgA_responses_to_milk_allergens_ L2 - https://doi.org/10.1111/j.1398-9995.2011.02635.x DB - PRIME DP - Unbound Medicine ER -