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Evaluation of hydro-alcoholic extract of Eclipta alba for its anticancer potential: an in vitro study.
J Ethnopharmacol. 2011 Jun 22; 136(2):363-7.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Eclipta alba is traditionally used as hepatoprotective agent. The study was designed to explore its antiproliferative activity on liver and other related cancer.

AIM OF THE STUDY

The present study was designed to assess and establish the role of Eclipta alba as anti-cancer agent using HepG2, C6 glioma and A498 cell lines as model system.

MATERIALS AND METHODS

Antiproliferative and cytotoxic effects of the Eclipta alba hydroalcoholic extract (EAE) was determined using MTT assay. The expression level of NF-kB was analysed by western blotting and RT PCR. Gelatin zymography was done for gelatinase matrix metalloproteinases (MMP-2 and 9) analysis.

RESULTS

EAE inhibited the cell proliferation in dose dependent manner in HepG2, A498 and C6 glioma cell lines with an IC50 of 22±2.9, 25±3.6 and 50±8.7 μg/ml, respectively. The expression of MMP (2 and 9) was down-regulated with EAE treatment. DNA damage was observed following 72h of extract treatment, leading to apoptosis. Additionally, the expression level of NF-kB was evaluated with western blotting and RT-PCR and was found to be down-regulated/inactivated.

CONCLUSIONS

The data establish the existence of anti-proliferative, DNA damaging and anti-metastasis properties in EAE which is yet unexplored and hold high therapeutic impact.

Authors+Show Affiliations

Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, Village - Chharodi, Ahmedabad, Gujarat 382481, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21575697

Citation

Chaudhary, Harshita, et al. "Evaluation of Hydro-alcoholic Extract of Eclipta Alba for Its Anticancer Potential: an in Vitro Study." Journal of Ethnopharmacology, vol. 136, no. 2, 2011, pp. 363-7.
Chaudhary H, Dhuna V, Singh J, et al. Evaluation of hydro-alcoholic extract of Eclipta alba for its anticancer potential: an in vitro study. J Ethnopharmacol. 2011;136(2):363-7.
Chaudhary, H., Dhuna, V., Singh, J., Kamboj, S. S., & Seshadri, S. (2011). Evaluation of hydro-alcoholic extract of Eclipta alba for its anticancer potential: an in vitro study. Journal of Ethnopharmacology, 136(2), 363-7. https://doi.org/10.1016/j.jep.2011.04.066
Chaudhary H, et al. Evaluation of Hydro-alcoholic Extract of Eclipta Alba for Its Anticancer Potential: an in Vitro Study. J Ethnopharmacol. 2011 Jun 22;136(2):363-7. PubMed PMID: 21575697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of hydro-alcoholic extract of Eclipta alba for its anticancer potential: an in vitro study. AU - Chaudhary,Harshita, AU - Dhuna,Vikram, AU - Singh,Jatinder, AU - Kamboj,Sukhdev Singh, AU - Seshadri,Sriram, Y1 - 2011/05/06/ PY - 2011/02/15/received PY - 2011/04/03/revised PY - 2011/04/30/accepted PY - 2011/5/18/entrez PY - 2011/5/18/pubmed PY - 2011/12/15/medline SP - 363 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 136 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Eclipta alba is traditionally used as hepatoprotective agent. The study was designed to explore its antiproliferative activity on liver and other related cancer. AIM OF THE STUDY: The present study was designed to assess and establish the role of Eclipta alba as anti-cancer agent using HepG2, C6 glioma and A498 cell lines as model system. MATERIALS AND METHODS: Antiproliferative and cytotoxic effects of the Eclipta alba hydroalcoholic extract (EAE) was determined using MTT assay. The expression level of NF-kB was analysed by western blotting and RT PCR. Gelatin zymography was done for gelatinase matrix metalloproteinases (MMP-2 and 9) analysis. RESULTS: EAE inhibited the cell proliferation in dose dependent manner in HepG2, A498 and C6 glioma cell lines with an IC50 of 22±2.9, 25±3.6 and 50±8.7 μg/ml, respectively. The expression of MMP (2 and 9) was down-regulated with EAE treatment. DNA damage was observed following 72h of extract treatment, leading to apoptosis. Additionally, the expression level of NF-kB was evaluated with western blotting and RT-PCR and was found to be down-regulated/inactivated. CONCLUSIONS: The data establish the existence of anti-proliferative, DNA damaging and anti-metastasis properties in EAE which is yet unexplored and hold high therapeutic impact. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21575697/Evaluation_of_hydro_alcoholic_extract_of_Eclipta_alba_for_its_anticancer_potential:_an_in_vitro_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00320-5 DB - PRIME DP - Unbound Medicine ER -