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Identification of the chromosome complement and the spontaneous 1R/1V translocations in allotetraploid Secale cereale × Dasypyrum villosum hybrids through cytogenetic approaches.
J Appl Genet. 2011 Aug; 52(3):305-11.JA

Abstract

Genome modifications that occur at the initial interspecific hybridization event are dynamic and can be consolidated during the process of stabilization in successive generations of allopolyploids. This study identifies the number and chromosomal location of ribosomal DNA (rDNA) sites between Secale cereale, Dasypyrum villosum, and their allotetraploid S. cereale × D. villosum hybrids. For the first time, we show the advantages of FISH to reveal chromosome rearrangements in the tetraploid Secale × Dasypyrum hybrids. Based on the specific hybridization patterns of ribosomal 5S, 35S DNA and rye species-specific pSc200 DNA probes, a set of genotypes with numerous Secale/Dasypyrum translocations of 1R/1V chromosomes were identified in successive generations of allotetraploid S. cereale × D. villosum hybrids. In addition we analyse rye chromosome pairs using FISH with chromosome-specific DNA sequences on S. cereale × D. villosum hybrids.

Authors+Show Affiliations

Laboratory of Cytogenetics and Molecular Biology, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, Poznan, Poland. tksi@igr.poznan.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21584731

Citation

Książczyk, Tomasz, et al. "Identification of the Chromosome Complement and the Spontaneous 1R/1V Translocations in Allotetraploid Secale Cereale × Dasypyrum Villosum Hybrids Through Cytogenetic Approaches." Journal of Applied Genetics, vol. 52, no. 3, 2011, pp. 305-11.
Książczyk T, Apolinarska B, Kulak-Książczyk S, et al. Identification of the chromosome complement and the spontaneous 1R/1V translocations in allotetraploid Secale cereale × Dasypyrum villosum hybrids through cytogenetic approaches. J Appl Genet. 2011;52(3):305-11.
Książczyk, T., Apolinarska, B., Kulak-Książczyk, S., Wiśniewska, H., Stojałowski, S., & Łapiński, M. (2011). Identification of the chromosome complement and the spontaneous 1R/1V translocations in allotetraploid Secale cereale × Dasypyrum villosum hybrids through cytogenetic approaches. Journal of Applied Genetics, 52(3), 305-11. https://doi.org/10.1007/s13353-011-0048-y
Książczyk T, et al. Identification of the Chromosome Complement and the Spontaneous 1R/1V Translocations in Allotetraploid Secale Cereale × Dasypyrum Villosum Hybrids Through Cytogenetic Approaches. J Appl Genet. 2011;52(3):305-11. PubMed PMID: 21584731.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of the chromosome complement and the spontaneous 1R/1V translocations in allotetraploid Secale cereale × Dasypyrum villosum hybrids through cytogenetic approaches. AU - Książczyk,Tomasz, AU - Apolinarska,Barbara, AU - Kulak-Książczyk,Sylwia, AU - Wiśniewska,Halina, AU - Stojałowski,Stefan, AU - Łapiński,Mirosław, Y1 - 2011/05/17/ PY - 2011/01/28/received PY - 2011/04/07/accepted PY - 2011/03/27/revised PY - 2011/5/18/entrez PY - 2011/5/18/pubmed PY - 2011/12/13/medline SP - 305 EP - 11 JF - Journal of applied genetics JO - J Appl Genet VL - 52 IS - 3 N2 - Genome modifications that occur at the initial interspecific hybridization event are dynamic and can be consolidated during the process of stabilization in successive generations of allopolyploids. This study identifies the number and chromosomal location of ribosomal DNA (rDNA) sites between Secale cereale, Dasypyrum villosum, and their allotetraploid S. cereale × D. villosum hybrids. For the first time, we show the advantages of FISH to reveal chromosome rearrangements in the tetraploid Secale × Dasypyrum hybrids. Based on the specific hybridization patterns of ribosomal 5S, 35S DNA and rye species-specific pSc200 DNA probes, a set of genotypes with numerous Secale/Dasypyrum translocations of 1R/1V chromosomes were identified in successive generations of allotetraploid S. cereale × D. villosum hybrids. In addition we analyse rye chromosome pairs using FISH with chromosome-specific DNA sequences on S. cereale × D. villosum hybrids. SN - 2190-3883 UR - https://www.unboundmedicine.com/medline/citation/21584731/Identification_of_the_chromosome_complement_and_the_spontaneous_1R/1V_translocations_in_allotetraploid_Secale_cereale_×_Dasypyrum_villosum_hybrids_through_cytogenetic_approaches_ L2 - https://dx.doi.org/10.1007/s13353-011-0048-y DB - PRIME DP - Unbound Medicine ER -