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[Influence of electroacupuncture on efficacy of estrogen in regulating hypothalamic reproductive endocrine activity in rats].
Zhen Ci Yan Jiu. 2011 Feb; 36(1):1-6, 39.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on the efficacy of estrogen-induced regulation of reproductive endocrine in ovariectomized (OVX) rats.

METHODS

Thirty SD rats were randomly divided into control, model (OVX), EA-Guanyuan(CV 4), Estrogen (E) and EA+ E groups (n = 6 in each group). OVX model was duplicated by removing the bilateral ovaries. EA (5 Hz/20 Hz, 1- 2 mA) was applied to "Guanyuan" (CV 4) for 30 min, once every other day and for 3 sessions. Estrogen at doses of 0.5 mg/kg and 0. 25 mg/kg was intraperitonealy given to rats in the E group and EA+ E group, respectively. Serum estrogen and cortisol contents were assayed by enzyme linked immunosorbent assay and the expression of hypothalamic gonadotrophin releasing hormone(GnRH)mRNA, G protein-coupled receptor 54(GPR 54)mRNA, kisspeptin-1 (Kiss-l) mRNA and corticotropin releasing hormone (CRH) mRNA were detected by using real time polymerase chain reaction (RT-PCR).

RESULTS

Compared with the control group, the expression levels of GnRH mRNA, GPR 54 mRNA and Kiss-1 mRNA in hypothalamus in the model group were increased significantly (P<0. 05), while the expression of hypothalamic CRH mRNA was down-regulated obviously (P<0. 05), and serum cortisol and estrogen levels were reduced significantly (P<0. 05). In comparison with the model group, the expression levels of hypothalamic GnRH mRNA in the EA, E and EA+ E groups, and those of hypothalamic GPR 54 mRNA and Kiss-1 mRNA in the E and EA+ E groups were down-regulated considerably (P<0. 05); whereas hypothalamic CRH mRNA expression levels in the E and EA+ E groups were up-regulated obviously (P<0. 05),and serum cortisol and estrogen contents in the E and EA+ E groups were increased remarkably (P<0.05). Comparison among the EA and EA+ E groups showed that the effects of EA+ E group were significantly superior to those of EA group in down-regulating hypothalalmic GnRH mRNA and GPR 54 mRNA expression, and in up-regulating hypothalamic CRH mRNA expression and serum cortisol and estrogen contents (P<0.05). No significant differences were found between E and EA + E groups in down-regulating hypothalamic GnRH mRNA, GPR 54 mRNA and Kiss-1 mRNA expression, and in up-regulating hypothalamic CRH mRNA expression and serum cortisol and estrogen levels (P>0. 05).

CONCLUSION

EA-CV 4 is able to potentiate the effects of low-dose of estrogen in down-regulating hypothalamic GnRH mRNA,GPR 54 mRNA and Kiss-1 mRNA expression, and up-regulating hypothalamic CRH mRNA expression and raising serum cortisol and estrogen contents in OVX rats, showing a favorable modulation effect on reproductive endocrine activity.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beiing 100700, China. ddwsj5@sina.comNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21585050

Citation

Wang, Shao-jun, et al. "[Influence of Electroacupuncture On Efficacy of Estrogen in Regulating Hypothalamic Reproductive Endocrine Activity in Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 36, no. 1, 2011, pp. 1-6, 39.
Wang SJ, Tan LH, Yang YS. [Influence of electroacupuncture on efficacy of estrogen in regulating hypothalamic reproductive endocrine activity in rats]. Zhen Ci Yan Jiu. 2011;36(1):1-6, 39.
Wang, S. J., Tan, L. H., & Yang, Y. S. (2011). [Influence of electroacupuncture on efficacy of estrogen in regulating hypothalamic reproductive endocrine activity in rats]. Zhen Ci Yan Jiu = Acupuncture Research, 36(1), 1-6, 39.
Wang SJ, Tan LH, Yang YS. [Influence of Electroacupuncture On Efficacy of Estrogen in Regulating Hypothalamic Reproductive Endocrine Activity in Rats]. Zhen Ci Yan Jiu. 2011;36(1):1-6, 39. PubMed PMID: 21585050.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Influence of electroacupuncture on efficacy of estrogen in regulating hypothalamic reproductive endocrine activity in rats]. AU - Wang,Shao-jun, AU - Tan,Lian-hong, AU - Yang,Yong-sheng, PY - 2011/5/19/entrez PY - 2011/5/19/pubmed PY - 2011/7/13/medline SP - 1-6, 39 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 36 IS - 1 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on the efficacy of estrogen-induced regulation of reproductive endocrine in ovariectomized (OVX) rats. METHODS: Thirty SD rats were randomly divided into control, model (OVX), EA-Guanyuan(CV 4), Estrogen (E) and EA+ E groups (n = 6 in each group). OVX model was duplicated by removing the bilateral ovaries. EA (5 Hz/20 Hz, 1- 2 mA) was applied to "Guanyuan" (CV 4) for 30 min, once every other day and for 3 sessions. Estrogen at doses of 0.5 mg/kg and 0. 25 mg/kg was intraperitonealy given to rats in the E group and EA+ E group, respectively. Serum estrogen and cortisol contents were assayed by enzyme linked immunosorbent assay and the expression of hypothalamic gonadotrophin releasing hormone(GnRH)mRNA, G protein-coupled receptor 54(GPR 54)mRNA, kisspeptin-1 (Kiss-l) mRNA and corticotropin releasing hormone (CRH) mRNA were detected by using real time polymerase chain reaction (RT-PCR). RESULTS: Compared with the control group, the expression levels of GnRH mRNA, GPR 54 mRNA and Kiss-1 mRNA in hypothalamus in the model group were increased significantly (P<0. 05), while the expression of hypothalamic CRH mRNA was down-regulated obviously (P<0. 05), and serum cortisol and estrogen levels were reduced significantly (P<0. 05). In comparison with the model group, the expression levels of hypothalamic GnRH mRNA in the EA, E and EA+ E groups, and those of hypothalamic GPR 54 mRNA and Kiss-1 mRNA in the E and EA+ E groups were down-regulated considerably (P<0. 05); whereas hypothalamic CRH mRNA expression levels in the E and EA+ E groups were up-regulated obviously (P<0. 05),and serum cortisol and estrogen contents in the E and EA+ E groups were increased remarkably (P<0.05). Comparison among the EA and EA+ E groups showed that the effects of EA+ E group were significantly superior to those of EA group in down-regulating hypothalalmic GnRH mRNA and GPR 54 mRNA expression, and in up-regulating hypothalamic CRH mRNA expression and serum cortisol and estrogen contents (P<0.05). No significant differences were found between E and EA + E groups in down-regulating hypothalamic GnRH mRNA, GPR 54 mRNA and Kiss-1 mRNA expression, and in up-regulating hypothalamic CRH mRNA expression and serum cortisol and estrogen levels (P>0. 05). CONCLUSION: EA-CV 4 is able to potentiate the effects of low-dose of estrogen in down-regulating hypothalamic GnRH mRNA,GPR 54 mRNA and Kiss-1 mRNA expression, and up-regulating hypothalamic CRH mRNA expression and raising serum cortisol and estrogen contents in OVX rats, showing a favorable modulation effect on reproductive endocrine activity. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/21585050/[Influence_of_electroacupuncture_on_efficacy_of_estrogen_in_regulating_hypothalamic_reproductive_endocrine_activity_in_rats]_ L2 - http://amigo.geneontology.org/amigo/reference/PMID:21585050 DB - PRIME DP - Unbound Medicine ER -