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[Effect of "Xiusanzhen"on expression of hippocampal Bcl-2 and Bax proteins in Alzheimer disease rats].
Zhen Ci Yan Jiu. 2011 Feb; 36(1):7-11.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) of "Xiusanzhen" [3 acupoints, i.e., bilateral "Yingxiang" (LI 20) and "Yintang" (EX-HN 3)] on expression of B-cell lymphoma/leukemia-2 (Bcl-2, an antiapoptosis protein) and Bax (a protein for promoting apoptosis) in the hippocampus in Alzheimer disease (AD) rats, so as to explore its clinical mechanisms underlying relieving AD.

METHODS

A total of 40 Sprague Dawley rats were randomly divided into normal control, AD model, EA and olfactory nerve transaction(ONT) + EA groups, with 10 rats in each. AD model was established by injection of beta-amyloid (Abeta)(1-40) (2 microL containing 10 microg) into the hippocampus (Bregma: 3.5 mm, L: 2.0 mm, H: -2.8 mm below dura), and the ON was sectioned by using a fine needle. EA (80-100 Hz, 1-3 mA) was applied to bilateral LI 20 and EX-HN 3 for 10 min, once daily (except Saturdays and Sundays) for 6 weeks. The expression of Bcl-2 and Bax of hippocampus was measured by immunohisto-chemistry.

RESULTS

Compared with the normal control group, the expression of Bcl-2 of hippocampus was significantly lower in the AD model group (P<0.05), and that of Bax in AD model group was much higher (P<0.01). In comparison with the model group, the expression of hippocampal Bcl-2 was up-regulated significantly (P<0.01), and that of hippocampal Bax protein downregulated considerably in the EA group (P<0.01). No significant differences were found between AD model and ONT + EA groups in the expression of hippocampal Bcl-2 and Bax proteins (P>0.05), suggesting a necessary premise condition for EA in regulating hippocampal Bcl-2 and Bax expression.

CONCLUSION

"Xiusanzhen" can regulate the expression of hippocampal Bcl-2 and Bax proteins in AD rats, which may contribute to its clinical effect in relieving AD, and the therapeutic effect depends on the integrity of the olfactory nerve pathway.

Authors+Show Affiliations

Department of Acu-moxibustion and Tuina, Shaanxi College of Chinese Medicine, Xianyang 712046, China. lzb210396@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21585051

Citation

Liu, Zhi-bin, et al. "[Effect of "Xiusanzhen"on Expression of Hippocampal Bcl-2 and Bax Proteins in Alzheimer Disease Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 36, no. 1, 2011, pp. 7-11.
Liu ZB, Niu WM, Yang XB, et al. [Effect of "Xiusanzhen"on expression of hippocampal Bcl-2 and Bax proteins in Alzheimer disease rats]. Zhen Ci Yan Jiu. 2011;36(1):7-11.
Liu, Z. B., Niu, W. M., Yang, X. B., Niu, X. M., & Yuan, W. (2011). [Effect of "Xiusanzhen"on expression of hippocampal Bcl-2 and Bax proteins in Alzheimer disease rats]. Zhen Ci Yan Jiu = Acupuncture Research, 36(1), 7-11.
Liu ZB, et al. [Effect of "Xiusanzhen"on Expression of Hippocampal Bcl-2 and Bax Proteins in Alzheimer Disease Rats]. Zhen Ci Yan Jiu. 2011;36(1):7-11. PubMed PMID: 21585051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of "Xiusanzhen"on expression of hippocampal Bcl-2 and Bax proteins in Alzheimer disease rats]. AU - Liu,Zhi-bin, AU - Niu,Wen-min, AU - Yang,Xiao-bang, AU - Niu,Xiao-mei, AU - Yuan,Wang, PY - 2011/5/19/entrez PY - 2011/5/19/pubmed PY - 2011/7/13/medline SP - 7 EP - 11 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 36 IS - 1 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) of "Xiusanzhen" [3 acupoints, i.e., bilateral "Yingxiang" (LI 20) and "Yintang" (EX-HN 3)] on expression of B-cell lymphoma/leukemia-2 (Bcl-2, an antiapoptosis protein) and Bax (a protein for promoting apoptosis) in the hippocampus in Alzheimer disease (AD) rats, so as to explore its clinical mechanisms underlying relieving AD. METHODS: A total of 40 Sprague Dawley rats were randomly divided into normal control, AD model, EA and olfactory nerve transaction(ONT) + EA groups, with 10 rats in each. AD model was established by injection of beta-amyloid (Abeta)(1-40) (2 microL containing 10 microg) into the hippocampus (Bregma: 3.5 mm, L: 2.0 mm, H: -2.8 mm below dura), and the ON was sectioned by using a fine needle. EA (80-100 Hz, 1-3 mA) was applied to bilateral LI 20 and EX-HN 3 for 10 min, once daily (except Saturdays and Sundays) for 6 weeks. The expression of Bcl-2 and Bax of hippocampus was measured by immunohisto-chemistry. RESULTS: Compared with the normal control group, the expression of Bcl-2 of hippocampus was significantly lower in the AD model group (P<0.05), and that of Bax in AD model group was much higher (P<0.01). In comparison with the model group, the expression of hippocampal Bcl-2 was up-regulated significantly (P<0.01), and that of hippocampal Bax protein downregulated considerably in the EA group (P<0.01). No significant differences were found between AD model and ONT + EA groups in the expression of hippocampal Bcl-2 and Bax proteins (P>0.05), suggesting a necessary premise condition for EA in regulating hippocampal Bcl-2 and Bax expression. CONCLUSION: "Xiusanzhen" can regulate the expression of hippocampal Bcl-2 and Bax proteins in AD rats, which may contribute to its clinical effect in relieving AD, and the therapeutic effect depends on the integrity of the olfactory nerve pathway. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/21585051/[Effect_of_"Xiusanzhen"on_expression_of_hippocampal_Bcl_2_and_Bax_proteins_in_Alzheimer_disease_rats]_ L2 - http://www.diseaseinfosearch.org/result/349 DB - PRIME DP - Unbound Medicine ER -