Tags

Type your tag names separated by a space and hit enter

Effect of second-messenger modulators in K-562 cell differentiation: dual action of calcium/phospholipid-dependent protein kinase in the process of differentiation.
J Cell Physiol. 1990 May; 143(2):243-50.JC

Abstract

We investigated the roles of second messengers in K-562 cell differentiation induced by either commitment-inducing agents (Ara-C, thymidine), or a noncommitment-inducing agent (hemin). Cell differentiation induced by both types of agents was inhibited by dbc-AMP, staurosporine, and H-7. In contrast, OAG enhanced hemin-induced cell differentiation, but it inhibited that due to Ara-C or thymidine. When K-562 cells were incubated with 4 x 10(-6)M hemin or 2 x 10(-7)M Ara-C for 2 days, an increase of epsilon-mRNA occurred. The addition of cycloheximide (1 microgram/ml) completely blocked this change, suggesting that de novo protein synthesis was necessary for the increase of epsilon-mRNA. Simultaneous treatment with Ara-C and cycloheximide for 2 days did not block either the increase of epsilon-mRNA or that of benzidine-positive cells, which were measured after 5 days of further incubation without additives. This suggested that the process of Ara-C-induced K-562 cell differentiation could be divided into two steps, i.e., a commitment step and a phenotypic expression step, and that the commitment step was at least partly resistant to cycloheximide. We investigated the roles of second messengers in each step. Our results suggested that PKC may act as a negative regulator of commitment step and as a positive regulator of the phenotypic expression. This may explain the differing effects of OAG on hemin- and Ara-C-induced K-562 cell differentiation.

Authors+Show Affiliations

Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2159013

Citation

Yumoto, Y, et al. "Effect of Second-messenger Modulators in K-562 Cell Differentiation: Dual Action of Calcium/phospholipid-dependent Protein Kinase in the Process of Differentiation." Journal of Cellular Physiology, vol. 143, no. 2, 1990, pp. 243-50.
Yumoto Y, Tashima M, Kato Y, et al. Effect of second-messenger modulators in K-562 cell differentiation: dual action of calcium/phospholipid-dependent protein kinase in the process of differentiation. J Cell Physiol. 1990;143(2):243-50.
Yumoto, Y., Tashima, M., Kato, Y., Ueda, T., Okuda, T., Ogawa, K., & Sawada, H. (1990). Effect of second-messenger modulators in K-562 cell differentiation: dual action of calcium/phospholipid-dependent protein kinase in the process of differentiation. Journal of Cellular Physiology, 143(2), 243-50.
Yumoto Y, et al. Effect of Second-messenger Modulators in K-562 Cell Differentiation: Dual Action of Calcium/phospholipid-dependent Protein Kinase in the Process of Differentiation. J Cell Physiol. 1990;143(2):243-50. PubMed PMID: 2159013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of second-messenger modulators in K-562 cell differentiation: dual action of calcium/phospholipid-dependent protein kinase in the process of differentiation. AU - Yumoto,Y, AU - Tashima,M, AU - Kato,Y, AU - Ueda,T, AU - Okuda,T, AU - Ogawa,K, AU - Sawada,H, PY - 1990/5/1/pubmed PY - 1990/5/1/medline PY - 1990/5/1/entrez SP - 243 EP - 50 JF - Journal of cellular physiology JO - J Cell Physiol VL - 143 IS - 2 N2 - We investigated the roles of second messengers in K-562 cell differentiation induced by either commitment-inducing agents (Ara-C, thymidine), or a noncommitment-inducing agent (hemin). Cell differentiation induced by both types of agents was inhibited by dbc-AMP, staurosporine, and H-7. In contrast, OAG enhanced hemin-induced cell differentiation, but it inhibited that due to Ara-C or thymidine. When K-562 cells were incubated with 4 x 10(-6)M hemin or 2 x 10(-7)M Ara-C for 2 days, an increase of epsilon-mRNA occurred. The addition of cycloheximide (1 microgram/ml) completely blocked this change, suggesting that de novo protein synthesis was necessary for the increase of epsilon-mRNA. Simultaneous treatment with Ara-C and cycloheximide for 2 days did not block either the increase of epsilon-mRNA or that of benzidine-positive cells, which were measured after 5 days of further incubation without additives. This suggested that the process of Ara-C-induced K-562 cell differentiation could be divided into two steps, i.e., a commitment step and a phenotypic expression step, and that the commitment step was at least partly resistant to cycloheximide. We investigated the roles of second messengers in each step. Our results suggested that PKC may act as a negative regulator of commitment step and as a positive regulator of the phenotypic expression. This may explain the differing effects of OAG on hemin- and Ara-C-induced K-562 cell differentiation. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/2159013/Effect_of_second_messenger_modulators_in_K_562_cell_differentiation:_dual_action_of_calcium/phospholipid_dependent_protein_kinase_in_the_process_of_differentiation_ L2 - https://doi.org/10.1002/jcp.1041430207 DB - PRIME DP - Unbound Medicine ER -