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Antioxidant and antimicrobial activities of branches extracts of five Juniperus species from Turkey.
Pharm Biol. 2011 Oct; 49(10):1014-22.PB

Abstract

CONTEXT

Several Juniperus species (Cupressaceae) are utilized in folk medicine in the treatment of infections and skin diseases.

OBJECTIVE

This work was designed to evaluate the antioxidant and antimicrobial potential of methanol and water branches extracts of Juniperus species from Turkey: Juniperus communis L. var. communis (Jcc), Juniperus communis L. var. saxatilis Pall. (Jcs), Juniperus drupacea Labill. (Jd), Juniperus oxycedrus L. subsp. oxycedrus (Joo), Juniperus oxycedrus L. subsp. macrocarpa (Sibth. & Sm.) Ball. (Jom).

MATERIALS AND METHODS

Total phenolics, total flavonoids and condensed tannins were spectrophotometrically determined. The antioxidant properties were examined using different in vitro systems. The toxicity was assayed by Artemia salina lethality test. The antimicrobial potential against bacteria and yeasts was evaluated using minimum inhibitory concentration and minimum bactericidal concentration (MIC/MBC) measurements. The effect on bacteria biofilms was tested by microtiter plate assay.

RESULTS

Both in the DPPH (1,1-diphenyl-2-picrylhydrazyl) and TBA (thiobarbituric acid) test Jom resulted the most active (IC(50) = 0.034 ± 0.002 mg/mL and 0.287 ± 0.166 µg/mL). Joo exhibited the highest reducing power (1.78 ± 0.04 ASE/mL) and Fe(2+) chelating activity (IC(50) = 0.537 ± 0.006 mg/mL). A positive correlation between primary antioxidant activity and phenolic content was found. The extracts were potentially non-toxic against Artemia salina. They showed the best antimicrobial (MIC = 4.88-30.10 µg/mL) and anti-biofilm activity (60-84%) against S. aureus.

DISCUSSION AND CONCLUSION

The results give a scientific basis to the traditional utilization of these Juniperus species, also demonstrating their potential as sources of natural antioxidant and antimicrobial compounds.

Authors+Show Affiliations

Pharmaco-Biological Department, Faculty of Pharmacy, University of Messina, Messina, Italy. mftaviano@pharma.unime.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21592011

Citation

Taviano, Maria Fernanda, et al. "Antioxidant and Antimicrobial Activities of Branches Extracts of Five Juniperus Species From Turkey." Pharmaceutical Biology, vol. 49, no. 10, 2011, pp. 1014-22.
Taviano MF, Marino A, Trovato A, et al. Antioxidant and antimicrobial activities of branches extracts of five Juniperus species from Turkey. Pharm Biol. 2011;49(10):1014-22.
Taviano, M. F., Marino, A., Trovato, A., Bellinghieri, V., La Barbera, T. M., Güvenç, A., Hürkul, M. M., Pasquale, R. D., & Miceli, N. (2011). Antioxidant and antimicrobial activities of branches extracts of five Juniperus species from Turkey. Pharmaceutical Biology, 49(10), 1014-22. https://doi.org/10.3109/13880209.2011.560161
Taviano MF, et al. Antioxidant and Antimicrobial Activities of Branches Extracts of Five Juniperus Species From Turkey. Pharm Biol. 2011;49(10):1014-22. PubMed PMID: 21592011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and antimicrobial activities of branches extracts of five Juniperus species from Turkey. AU - Taviano,Maria Fernanda, AU - Marino,Andreana, AU - Trovato,Ada, AU - Bellinghieri,Valentina, AU - La Barbera,Tommaso Massimo, AU - Güvenç,Ayşegül, AU - Hürkul,Muhammed Mesud, AU - Pasquale,Rita De, AU - Miceli,Natalizia, Y1 - 2011/05/19/ PY - 2011/5/20/entrez PY - 2011/5/20/pubmed PY - 2012/4/17/medline SP - 1014 EP - 22 JF - Pharmaceutical biology JO - Pharm Biol VL - 49 IS - 10 N2 - CONTEXT: Several Juniperus species (Cupressaceae) are utilized in folk medicine in the treatment of infections and skin diseases. OBJECTIVE: This work was designed to evaluate the antioxidant and antimicrobial potential of methanol and water branches extracts of Juniperus species from Turkey: Juniperus communis L. var. communis (Jcc), Juniperus communis L. var. saxatilis Pall. (Jcs), Juniperus drupacea Labill. (Jd), Juniperus oxycedrus L. subsp. oxycedrus (Joo), Juniperus oxycedrus L. subsp. macrocarpa (Sibth. & Sm.) Ball. (Jom). MATERIALS AND METHODS: Total phenolics, total flavonoids and condensed tannins were spectrophotometrically determined. The antioxidant properties were examined using different in vitro systems. The toxicity was assayed by Artemia salina lethality test. The antimicrobial potential against bacteria and yeasts was evaluated using minimum inhibitory concentration and minimum bactericidal concentration (MIC/MBC) measurements. The effect on bacteria biofilms was tested by microtiter plate assay. RESULTS: Both in the DPPH (1,1-diphenyl-2-picrylhydrazyl) and TBA (thiobarbituric acid) test Jom resulted the most active (IC(50) = 0.034 ± 0.002 mg/mL and 0.287 ± 0.166 µg/mL). Joo exhibited the highest reducing power (1.78 ± 0.04 ASE/mL) and Fe(2+) chelating activity (IC(50) = 0.537 ± 0.006 mg/mL). A positive correlation between primary antioxidant activity and phenolic content was found. The extracts were potentially non-toxic against Artemia salina. They showed the best antimicrobial (MIC = 4.88-30.10 µg/mL) and anti-biofilm activity (60-84%) against S. aureus. DISCUSSION AND CONCLUSION: The results give a scientific basis to the traditional utilization of these Juniperus species, also demonstrating their potential as sources of natural antioxidant and antimicrobial compounds. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/21592011/Antioxidant_and_antimicrobial_activities_of_branches_extracts_of_five_Juniperus_species_from_Turkey_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2011.560161 DB - PRIME DP - Unbound Medicine ER -