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Bioelectric properties of fetal alveolar epithelial monolayers.
Am J Physiol 1990; 258(4 Pt 1):L201-6AJ

Abstract

To investigate the bioelectric properties on one of the cell types that line the distal lung unit, we isolated type II alveolar epithelium from 18- to 20-day gestation fetal rats (term = 22 days) and grew them on collagen-coated nitrocellulose filters. Amiloride impaired ion transport in a dose-dependent fashion (10(-4) to 10(-6) M) with 10(-4) M decreasing potential difference (PD) (mean +/- SE, 2.0 +/- 0.49 to 0.9 +/- 0.26 mV, P less than 0.01, lumen negative) and short-circuit current (Isc) (7.0 +/- 1.0 to 2.4 +/- 0.64 uA/cm2, P less than 0.01) without affecting resistance (R) (241 +/- 33 to 216 +/- 41 omega. cm2). Benzamil (10(-5) M) but not dimethylamiloride (10(-5) M) decreased Isc. Terbutaline (10(-3) M) increased PD from 1.2 +/- 0.13 to 3.3 +/- 0.40 mV (P less than 0.01), and application of amiloride (10(-4) M) after terbutaline reduced PD and Isc to less than initial base-line values. The Na(+)-K(+)-2Cl- cotransport inhibitors bumetanide (10(-4) M) and furosemide (10(-3) M) had no effect on PD and Isc either before or after terbutaline. Neither the Cl- channel blocker diphenylamine-2-carboxylate (10(-3) M) nor the Na(+)-glucose cotransport inhibitor phloridzin (10(-3) M) affected the bioelectric properties. Fetal type II alveolar epithelium in primary culture actively transport ions and, on the basis of inhibitor-agonist experiments, probably do not secrete Cl- but absorb Na+ through Na+ channels.

Authors+Show Affiliations

Department of Paediatrics, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2159225

Citation

O'Brodovich, H, et al. "Bioelectric Properties of Fetal Alveolar Epithelial Monolayers." The American Journal of Physiology, vol. 258, no. 4 Pt 1, 1990, pp. L201-6.
O'Brodovich H, Rafii B, Post M. Bioelectric properties of fetal alveolar epithelial monolayers. Am J Physiol. 1990;258(4 Pt 1):L201-6.
O'Brodovich, H., Rafii, B., & Post, M. (1990). Bioelectric properties of fetal alveolar epithelial monolayers. The American Journal of Physiology, 258(4 Pt 1), pp. L201-6.
O'Brodovich H, Rafii B, Post M. Bioelectric Properties of Fetal Alveolar Epithelial Monolayers. Am J Physiol. 1990;258(4 Pt 1):L201-6. PubMed PMID: 2159225.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioelectric properties of fetal alveolar epithelial monolayers. AU - O'Brodovich,H, AU - Rafii,B, AU - Post,M, PY - 1990/4/1/pubmed PY - 1990/4/1/medline PY - 1990/4/1/entrez SP - L201 EP - 6 JF - The American journal of physiology JO - Am. J. Physiol. VL - 258 IS - 4 Pt 1 N2 - To investigate the bioelectric properties on one of the cell types that line the distal lung unit, we isolated type II alveolar epithelium from 18- to 20-day gestation fetal rats (term = 22 days) and grew them on collagen-coated nitrocellulose filters. Amiloride impaired ion transport in a dose-dependent fashion (10(-4) to 10(-6) M) with 10(-4) M decreasing potential difference (PD) (mean +/- SE, 2.0 +/- 0.49 to 0.9 +/- 0.26 mV, P less than 0.01, lumen negative) and short-circuit current (Isc) (7.0 +/- 1.0 to 2.4 +/- 0.64 uA/cm2, P less than 0.01) without affecting resistance (R) (241 +/- 33 to 216 +/- 41 omega. cm2). Benzamil (10(-5) M) but not dimethylamiloride (10(-5) M) decreased Isc. Terbutaline (10(-3) M) increased PD from 1.2 +/- 0.13 to 3.3 +/- 0.40 mV (P less than 0.01), and application of amiloride (10(-4) M) after terbutaline reduced PD and Isc to less than initial base-line values. The Na(+)-K(+)-2Cl- cotransport inhibitors bumetanide (10(-4) M) and furosemide (10(-3) M) had no effect on PD and Isc either before or after terbutaline. Neither the Cl- channel blocker diphenylamine-2-carboxylate (10(-3) M) nor the Na(+)-glucose cotransport inhibitor phloridzin (10(-3) M) affected the bioelectric properties. Fetal type II alveolar epithelium in primary culture actively transport ions and, on the basis of inhibitor-agonist experiments, probably do not secrete Cl- but absorb Na+ through Na+ channels. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/2159225/Bioelectric_properties_of_fetal_alveolar_epithelial_monolayers_ L2 - http://journals.physiology.org/doi/full/10.1152/ajplung.1990.258.4.L201?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -