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Frequency and phylogeny of norovirus in diarrheic children in Istanbul, Turkey.
J Clin Virol. 2011 Jul; 51(3):160-4.JC

Abstract

BACKGROUND

Norovirus (NoV) is recognised as one of the most common causes of foodborne infections. Contaminated shellfish, food, water and hospitals are well documented sources of the virus.

OBJECTIVE

NoV in diarrheic children has not previously been investigated in Istanbul, Turkey, hence the aim of this study was to detect and investigate the frequency and phylogeny of human NoV genogroups I and II in children with acute gastroenteritis.

STUDY DESIGN

238 stool samples were collected from diarrheic children from 2 hospitals (Cerrahpasa Medical School and Haseki) in Istanbul and analysed by ELISA, RT-PCR and real-time RT-PCR using both SYBR Green and probe-based assays for human NoV. Primers targeting the RNA-polymerase gene were used for RT-PCR to allow DNA sequencing of Turkish NoV strains and phylogenetic analysis to be performed.

RESULTS

NoV GII was detected in 36 (15.1%) of 238 samples by SYBR Green real-time RT-PCR, 10.9% by a probe-based real-time RT-PCR and 10.5% by ELISA (Ridascreen). Genogroup II (GII) the Turkish NoVs clustered with including GII4 (72.2%), GII16 (5.5%), GIIb (16.7%) and GIIe (5.5%). Two variants of GII4 (GII4-2006b and GII4-2008), GII16 and recombinant noroviruses (GIIb and GIIe) were identified.

CONCLUSION

This study shows a high frequency and genetic diversity of NoV GII infections in children with acute gastroenteritis in Istanbul, Turkey.

Authors+Show Affiliations

Başkent University Hospital, Clinic of Child Health and Diseases, Altunizade, Istanbul, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21592853

Citation

Ozkul, A A., et al. "Frequency and Phylogeny of Norovirus in Diarrheic Children in Istanbul, Turkey." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 51, no. 3, 2011, pp. 160-4.
Ozkul AA, Kocazeybek BS, Turan N, et al. Frequency and phylogeny of norovirus in diarrheic children in Istanbul, Turkey. J Clin Virol. 2011;51(3):160-4.
Ozkul, A. A., Kocazeybek, B. S., Turan, N., Reuter, G., Bostan, K., Yilmaz, A., Altan, E., Uyunmaz, G., Karaköse, A. R., Muratoglu, K., Elevli, M., Helps, C. R., & Yilmaz, H. (2011). Frequency and phylogeny of norovirus in diarrheic children in Istanbul, Turkey. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 51(3), 160-4. https://doi.org/10.1016/j.jcv.2011.03.004
Ozkul AA, et al. Frequency and Phylogeny of Norovirus in Diarrheic Children in Istanbul, Turkey. J Clin Virol. 2011;51(3):160-4. PubMed PMID: 21592853.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Frequency and phylogeny of norovirus in diarrheic children in Istanbul, Turkey. AU - Ozkul,A A, AU - Kocazeybek,Bekir S, AU - Turan,Nuri, AU - Reuter,Gábor, AU - Bostan,Kamil, AU - Yilmaz,Aysun, AU - Altan,Eda, AU - Uyunmaz,Gulsah, AU - Karaköse,Ali Riza, AU - Muratoglu,Karlo, AU - Elevli,Murat, AU - Helps,Christopher R, AU - Yilmaz,Huseyin, Y1 - 2011/05/17/ PY - 2010/11/18/received PY - 2011/03/09/revised PY - 2011/03/14/accepted PY - 2011/5/20/entrez PY - 2011/5/20/pubmed PY - 2011/9/29/medline SP - 160 EP - 4 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 51 IS - 3 N2 - BACKGROUND: Norovirus (NoV) is recognised as one of the most common causes of foodborne infections. Contaminated shellfish, food, water and hospitals are well documented sources of the virus. OBJECTIVE: NoV in diarrheic children has not previously been investigated in Istanbul, Turkey, hence the aim of this study was to detect and investigate the frequency and phylogeny of human NoV genogroups I and II in children with acute gastroenteritis. STUDY DESIGN: 238 stool samples were collected from diarrheic children from 2 hospitals (Cerrahpasa Medical School and Haseki) in Istanbul and analysed by ELISA, RT-PCR and real-time RT-PCR using both SYBR Green and probe-based assays for human NoV. Primers targeting the RNA-polymerase gene were used for RT-PCR to allow DNA sequencing of Turkish NoV strains and phylogenetic analysis to be performed. RESULTS: NoV GII was detected in 36 (15.1%) of 238 samples by SYBR Green real-time RT-PCR, 10.9% by a probe-based real-time RT-PCR and 10.5% by ELISA (Ridascreen). Genogroup II (GII) the Turkish NoVs clustered with including GII4 (72.2%), GII16 (5.5%), GIIb (16.7%) and GIIe (5.5%). Two variants of GII4 (GII4-2006b and GII4-2008), GII16 and recombinant noroviruses (GIIb and GIIe) were identified. CONCLUSION: This study shows a high frequency and genetic diversity of NoV GII infections in children with acute gastroenteritis in Istanbul, Turkey. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/21592853/Frequency_and_phylogeny_of_norovirus_in_diarrheic_children_in_Istanbul_Turkey_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(11)00108-9 DB - PRIME DP - Unbound Medicine ER -