Tags

Type your tag names separated by a space and hit enter

Genotype diversity and distribution of Orientia tsutsugamushi causing scrub typhus in Thailand.
J Clin Microbiol. 2011 Jul; 49(7):2584-9.JC

Abstract

Scrub typhus, caused by antigenically disparate isolates of Orientia tsutsugamushi, is a widely distributed mite-borne human disease in the Asia Pacific region. Information regarding the heterogeneity of the immunodominant 56-kDa type-specific antigen (TSA) gene is crucial for the design and evaluation of scrub typhus-specific diagnostic assays and vaccines. Using indirect immunofluorescence assays (IFA) and PCR assays, O. tsutsugamushi was detected samples from rodents and patients with fever of unknown origin obtained from six provinces of Thailand during 2004 to 2007. Sequences were determined for a fragment of the 56-kDa TSA gene, and the relationship between these sequences and those previously determined were assessed. The phylogenetic analyses of partial 56-kDa TSA gene sequences demonstrated wide diversity and distribution of O. tsutsugamushi genotypes in Thailand. Furthermore, the genetic diversity grouped the scrub typhus agents into two commonly and five infrequently found genotypes within six provinces of Thailand. The two most commonly found genotypes of O. tsutsugamushi described in this study do not associate with the prototype strains that are widely used for the design and evaluation of diagnostic assays and vaccine candidates. Thus, these new genotypes should be considered for future scrub typhus assay and vaccine development.

Authors+Show Affiliations

Research Division, Armed Forces Research Institute of Medical Science, Bangkok 10400, Thailand. toonr@afrims.go.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21593255

Citation

Ruang-Areerate, Toon, et al. "Genotype Diversity and Distribution of Orientia Tsutsugamushi Causing Scrub Typhus in Thailand." Journal of Clinical Microbiology, vol. 49, no. 7, 2011, pp. 2584-9.
Ruang-Areerate T, Jeamwattanalert P, Rodkvamtook W, et al. Genotype diversity and distribution of Orientia tsutsugamushi causing scrub typhus in Thailand. J Clin Microbiol. 2011;49(7):2584-9.
Ruang-Areerate, T., Jeamwattanalert, P., Rodkvamtook, W., Richards, A. L., Sunyakumthorn, P., & Gaywee, J. (2011). Genotype diversity and distribution of Orientia tsutsugamushi causing scrub typhus in Thailand. Journal of Clinical Microbiology, 49(7), 2584-9. https://doi.org/10.1128/JCM.00355-11
Ruang-Areerate T, et al. Genotype Diversity and Distribution of Orientia Tsutsugamushi Causing Scrub Typhus in Thailand. J Clin Microbiol. 2011;49(7):2584-9. PubMed PMID: 21593255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotype diversity and distribution of Orientia tsutsugamushi causing scrub typhus in Thailand. AU - Ruang-Areerate,Toon, AU - Jeamwattanalert,Pimmada, AU - Rodkvamtook,Wuttikorn, AU - Richards,Allen L, AU - Sunyakumthorn,Piyanate, AU - Gaywee,Jariyanart, Y1 - 2011/05/18/ PY - 2011/5/20/entrez PY - 2011/5/20/pubmed PY - 2011/9/29/medline SP - 2584 EP - 9 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 49 IS - 7 N2 - Scrub typhus, caused by antigenically disparate isolates of Orientia tsutsugamushi, is a widely distributed mite-borne human disease in the Asia Pacific region. Information regarding the heterogeneity of the immunodominant 56-kDa type-specific antigen (TSA) gene is crucial for the design and evaluation of scrub typhus-specific diagnostic assays and vaccines. Using indirect immunofluorescence assays (IFA) and PCR assays, O. tsutsugamushi was detected samples from rodents and patients with fever of unknown origin obtained from six provinces of Thailand during 2004 to 2007. Sequences were determined for a fragment of the 56-kDa TSA gene, and the relationship between these sequences and those previously determined were assessed. The phylogenetic analyses of partial 56-kDa TSA gene sequences demonstrated wide diversity and distribution of O. tsutsugamushi genotypes in Thailand. Furthermore, the genetic diversity grouped the scrub typhus agents into two commonly and five infrequently found genotypes within six provinces of Thailand. The two most commonly found genotypes of O. tsutsugamushi described in this study do not associate with the prototype strains that are widely used for the design and evaluation of diagnostic assays and vaccine candidates. Thus, these new genotypes should be considered for future scrub typhus assay and vaccine development. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/21593255/Genotype_diversity_and_distribution_of_Orientia_tsutsugamushi_causing_scrub_typhus_in_Thailand_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=21593255 DB - PRIME DP - Unbound Medicine ER -