Flores, María Isabel Alarcón, et al. "QuEChERS-based Extraction Procedure for Multifamily Analysis of Phytohormones in Vegetables By UHPLC-MS/MS." Journal of Separation Science, vol. 34, no. 13, 2011, pp. 1517-24.
Flores MI, Romero-González R, Frenich AG, et al. QuEChERS-based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC-MS/MS. J Sep Sci. 2011;34(13):1517-24.
Flores, M. I., Romero-González, R., Frenich, A. G., & Vidal, J. L. (2011). QuEChERS-based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC-MS/MS. Journal of Separation Science, 34(13), 1517-24. https://doi.org/10.1002/jssc.201100093
Flores MI, et al. QuEChERS-based Extraction Procedure for Multifamily Analysis of Phytohormones in Vegetables By UHPLC-MS/MS. J Sep Sci. 2011;34(13):1517-24. PubMed PMID: 21595026.
TY - JOUR
T1 - QuEChERS-based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC-MS/MS.
AU - Flores,María Isabel Alarcón,
AU - Romero-González,Roberto,
AU - Frenich,Antonia Garrido,
AU - Vidal,José Luis Martínez,
Y1 - 2011/05/19/
PY - 2011/02/02/received
PY - 2011/03/21/revised
PY - 2011/04/02/accepted
PY - 2011/5/20/entrez
PY - 2011/5/20/pubmed
PY - 2011/10/11/medline
SP - 1517
EP - 24
JF - Journal of separation science
JO - J Sep Sci
VL - 34
IS - 13
N2 - A new method has been developed and validated for the simultaneous analysis of different phytohormones (auxins, cytokinins and gibberellins) in vegetables. The compounds were extracted using a QuEChERS-based method (acronym of quick, easy, cheap, effective, rugged and safe). The separation and determination of the selected phytohormones were carried out by ultra high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), using electrospray ionization source (ESI) in positive and negative ion modes. The method was validated and mean recoveries were evaluated at three concentration levels (50, 100 and 250 μg/kg), ranging from 75 to 110% at the three levels assayed. Intra- and interday precisions, expressed as relative standard deviations (RSDs), were lower than 20 and 25%, respectively. Limits of quantification (LOQs) were equal or lower than 10 μg/kg. The developed procedure was applied to seven courgette samples, and naphthylacetic acid, naphthylacetamide and benzyladenine were found in some of the analysed samples.
SN - 1615-9314
UR - https://www.unboundmedicine.com/medline/citation/21595026/QuEChERS_based_extraction_procedure_for_multifamily_analysis_of_phytohormones_in_vegetables_by_UHPLC_MS/MS_
L2 - https://doi.org/10.1002/jssc.201100093
DB - PRIME
DP - Unbound Medicine