TY - JOUR T1 - QuEChERS-based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC-MS/MS. AU - Flores,María Isabel Alarcón, AU - Romero-González,Roberto, AU - Frenich,Antonia Garrido, AU - Vidal,José Luis Martínez, Y1 - 2011/05/19/ PY - 2011/02/02/received PY - 2011/03/21/revised PY - 2011/04/02/accepted PY - 2011/5/20/entrez PY - 2011/5/20/pubmed PY - 2011/10/11/medline SP - 1517 EP - 24 JF - Journal of separation science JO - J Sep Sci VL - 34 IS - 13 N2 - A new method has been developed and validated for the simultaneous analysis of different phytohormones (auxins, cytokinins and gibberellins) in vegetables. The compounds were extracted using a QuEChERS-based method (acronym of quick, easy, cheap, effective, rugged and safe). The separation and determination of the selected phytohormones were carried out by ultra high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), using electrospray ionization source (ESI) in positive and negative ion modes. The method was validated and mean recoveries were evaluated at three concentration levels (50, 100 and 250 μg/kg), ranging from 75 to 110% at the three levels assayed. Intra- and interday precisions, expressed as relative standard deviations (RSDs), were lower than 20 and 25%, respectively. Limits of quantification (LOQs) were equal or lower than 10 μg/kg. The developed procedure was applied to seven courgette samples, and naphthylacetic acid, naphthylacetamide and benzyladenine were found in some of the analysed samples. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/21595026/QuEChERS_based_extraction_procedure_for_multifamily_analysis_of_phytohormones_in_vegetables_by_UHPLC_MS/MS_ L2 - https://doi.org/10.1002/jssc.201100093 DB - PRIME DP - Unbound Medicine ER -