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Hemostatic and toxinological diversities in venom of Micrurus tener tener, Micrurus fulvius fulvius and Micrurus isozonus coral snakes.
Toxicon. 2011 Jul; 58(1):35-45.T

Abstract

The coral snake Micrurus tener tener (Mtt) from the Elapidae family inhabits the southwestern United States and produces severe cases of envenomations. Although the majority of Mtt venom components are neurotoxins and phospholipase A₂s, this study demonstrated, by SDS-PAGE and molecular exclusion chromatography (MEC), that these venoms also contain high-molecular-weight proteins between 50 and 150 kDa that target the hemostatic system. The biological aspects of other Micrurus venoms were also studied, such as the LD₅₀s of Micrurus isozonus (from 0.52 to 0.61 mg/kg). A pool from these venoms presented a LD₅₀ of 0.57 mg/kg, Micrurus f. fulvius (Mff) and Mtt had LD₅₀s of 0.32 and 0.78 mg/kg, respectively. These venoms contained fibrino(geno)lytic activity, they inhibited platelet aggregation, as well as factor Xa and/or plasmin-like activities. M. isozonus venoms from different Venezuelan geographical regions inhibited ADP-induced platelet aggregation (from 50 to 68%). Micrurus tener tener venom from the United States was the most active with a 95.2% inhibitory effect. This venom showed thrombin-like activity on fibrinogen and human plasma. Fractions of Mtt showed fibrino(geno)lytic activity and inhibition on plasmin amidolytic activity. Several fractions degraded the fibrinogen Aα chains, and fractions F2 and F7 completely degraded both fibrinogen Aα and Bβ chains. To our knowledge, this is the first report on thrombin-like and fibrino(geno)lytic activity and plasmin or factor Xa inhibitors described in Micrurus venoms. Further purification and characterization of these Micrurus venom components could be of therapeutic use in the treatment of hemostatic disorders.

Authors+Show Affiliations

Laboratorio de Fisiopatología, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Apartado 20632, Caracas 1020A, Venezuela.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21596052

Citation

Salazar, Ana M., et al. "Hemostatic and Toxinological Diversities in Venom of Micrurus Tener Tener, Micrurus Fulvius Fulvius and Micrurus Isozonus Coral Snakes." Toxicon : Official Journal of the International Society On Toxinology, vol. 58, no. 1, 2011, pp. 35-45.
Salazar AM, Vivas J, Sánchez EE, et al. Hemostatic and toxinological diversities in venom of Micrurus tener tener, Micrurus fulvius fulvius and Micrurus isozonus coral snakes. Toxicon. 2011;58(1):35-45.
Salazar, A. M., Vivas, J., Sánchez, E. E., Rodríguez-Acosta, A., Ibarra, C., Gil, A., Carvajal, Z., Girón, M. E., Estrella, A., Navarrete, L. F., & Guerrero, B. (2011). Hemostatic and toxinological diversities in venom of Micrurus tener tener, Micrurus fulvius fulvius and Micrurus isozonus coral snakes. Toxicon : Official Journal of the International Society On Toxinology, 58(1), 35-45. https://doi.org/10.1016/j.toxicon.2011.04.020
Salazar AM, et al. Hemostatic and Toxinological Diversities in Venom of Micrurus Tener Tener, Micrurus Fulvius Fulvius and Micrurus Isozonus Coral Snakes. Toxicon. 2011;58(1):35-45. PubMed PMID: 21596052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hemostatic and toxinological diversities in venom of Micrurus tener tener, Micrurus fulvius fulvius and Micrurus isozonus coral snakes. AU - Salazar,Ana M, AU - Vivas,Jeilyn, AU - Sánchez,Elda E, AU - Rodríguez-Acosta,Alexis, AU - Ibarra,Carlos, AU - Gil,Amparo, AU - Carvajal,Zoila, AU - Girón,María E, AU - Estrella,Amalid, AU - Navarrete,Luis F, AU - Guerrero,Belsy, Y1 - 2011/05/08/ PY - 2010/12/06/received PY - 2011/04/21/revised PY - 2011/04/26/accepted PY - 2011/5/21/entrez PY - 2011/5/21/pubmed PY - 2011/12/14/medline SP - 35 EP - 45 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 58 IS - 1 N2 - The coral snake Micrurus tener tener (Mtt) from the Elapidae family inhabits the southwestern United States and produces severe cases of envenomations. Although the majority of Mtt venom components are neurotoxins and phospholipase A₂s, this study demonstrated, by SDS-PAGE and molecular exclusion chromatography (MEC), that these venoms also contain high-molecular-weight proteins between 50 and 150 kDa that target the hemostatic system. The biological aspects of other Micrurus venoms were also studied, such as the LD₅₀s of Micrurus isozonus (from 0.52 to 0.61 mg/kg). A pool from these venoms presented a LD₅₀ of 0.57 mg/kg, Micrurus f. fulvius (Mff) and Mtt had LD₅₀s of 0.32 and 0.78 mg/kg, respectively. These venoms contained fibrino(geno)lytic activity, they inhibited platelet aggregation, as well as factor Xa and/or plasmin-like activities. M. isozonus venoms from different Venezuelan geographical regions inhibited ADP-induced platelet aggregation (from 50 to 68%). Micrurus tener tener venom from the United States was the most active with a 95.2% inhibitory effect. This venom showed thrombin-like activity on fibrinogen and human plasma. Fractions of Mtt showed fibrino(geno)lytic activity and inhibition on plasmin amidolytic activity. Several fractions degraded the fibrinogen Aα chains, and fractions F2 and F7 completely degraded both fibrinogen Aα and Bβ chains. To our knowledge, this is the first report on thrombin-like and fibrino(geno)lytic activity and plasmin or factor Xa inhibitors described in Micrurus venoms. Further purification and characterization of these Micrurus venom components could be of therapeutic use in the treatment of hemostatic disorders. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/21596052/Hemostatic_and_toxinological_diversities_in_venom_of_Micrurus_tener_tener_Micrurus_fulvius_fulvius_and_Micrurus_isozonus_coral_snakes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(11)00155-3 DB - PRIME DP - Unbound Medicine ER -