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A highly sensitive and rapid organophosphate biosensor based on enhancement of CdS-decorated graphene nanocomposite.
Anal Chim Acta. 2011 Jun 10; 695(1-2):84-8.AC

Abstract

This work reports a rapid and sensitive organophosphates (OPs) amperometric biosensor based on acetylcholinesterase (AChE) immobilized on CdS-decorated graphene (CdS-G) nanocomposite. The as-prepared biosensor shows high affinity to acetylthiocholine (ATCl) with a Michaelis-Menten constant (K(m)) value of 0.24 mM. A rapid inhibition time (2 min) is obtained due to the integration of the CdS-G nanocomposite. Based on the inhibition of OPs on the enzymatic activity of the immobilized AChE, and used carbaryl as the model compound, the resulting biosensor exhibits excellent performance for OPs detection including good reproducibility, acceptable stability, and a reliable linear relationship between the inhibition and log[carbaryl] from 2 ng mL⁻¹ up to 2 μg mL⁻¹ with a detection limit of 0.7 ng mL⁻¹,which provides a new promising tool for analysis of enzyme inhibitors.

Authors+Show Affiliations

Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, China. wangkun@ujs.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21601034

Citation

Wang, Kun, et al. "A Highly Sensitive and Rapid Organophosphate Biosensor Based On Enhancement of CdS-decorated Graphene Nanocomposite." Analytica Chimica Acta, vol. 695, no. 1-2, 2011, pp. 84-8.
Wang K, Liu Q, Dai L, et al. A highly sensitive and rapid organophosphate biosensor based on enhancement of CdS-decorated graphene nanocomposite. Anal Chim Acta. 2011;695(1-2):84-8.
Wang, K., Liu, Q., Dai, L., Yan, J., Ju, C., Qiu, B., & Wu, X. (2011). A highly sensitive and rapid organophosphate biosensor based on enhancement of CdS-decorated graphene nanocomposite. Analytica Chimica Acta, 695(1-2), 84-8. https://doi.org/10.1016/j.aca.2011.03.042
Wang K, et al. A Highly Sensitive and Rapid Organophosphate Biosensor Based On Enhancement of CdS-decorated Graphene Nanocomposite. Anal Chim Acta. 2011 Jun 10;695(1-2):84-8. PubMed PMID: 21601034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A highly sensitive and rapid organophosphate biosensor based on enhancement of CdS-decorated graphene nanocomposite. AU - Wang,Kun, AU - Liu,Qian, AU - Dai,Lina, AU - Yan,Jiajia, AU - Ju,Chang, AU - Qiu,Baijing, AU - Wu,Xiangyang, Y1 - 2011/04/14/ PY - 2010/12/15/received PY - 2011/01/19/revised PY - 2011/03/23/accepted PY - 2011/5/24/entrez PY - 2011/5/24/pubmed PY - 2011/10/1/medline SP - 84 EP - 8 JF - Analytica chimica acta JO - Anal Chim Acta VL - 695 IS - 1-2 N2 - This work reports a rapid and sensitive organophosphates (OPs) amperometric biosensor based on acetylcholinesterase (AChE) immobilized on CdS-decorated graphene (CdS-G) nanocomposite. The as-prepared biosensor shows high affinity to acetylthiocholine (ATCl) with a Michaelis-Menten constant (K(m)) value of 0.24 mM. A rapid inhibition time (2 min) is obtained due to the integration of the CdS-G nanocomposite. Based on the inhibition of OPs on the enzymatic activity of the immobilized AChE, and used carbaryl as the model compound, the resulting biosensor exhibits excellent performance for OPs detection including good reproducibility, acceptable stability, and a reliable linear relationship between the inhibition and log[carbaryl] from 2 ng mL⁻¹ up to 2 μg mL⁻¹ with a detection limit of 0.7 ng mL⁻¹,which provides a new promising tool for analysis of enzyme inhibitors. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/21601034/A_highly_sensitive_and_rapid_organophosphate_biosensor_based_on_enhancement_of_CdS_decorated_graphene_nanocomposite_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(11)00442-9 DB - PRIME DP - Unbound Medicine ER -