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Th17 cells contribute to nontypeable Haemophilus influenzae-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and α-galactosylceramide.
Microbiol Immunol. 2011 Aug; 55(8):574-81.MI

Abstract

Nasal vaccination is an effective therapeutic means of preventing upper respiratory infection. Recently, nasal vaccination with P6 outer membrane protein of nontypeable Haemophilus influenzae (NTHi) and alpha-galactosylceramide (α-GalCer) was reported to induce NTHi-specific protective immunity. The present study investigated the role of the Th17 cells induced by nasal vaccination. Mice were immunized with P6 and α-GalCer, and their P6-specific immune responses were examined. Cytokine-producing cells were analyzed by flow cytometry, and expression of cytokines in P6-specific CD4+ T cells was determined by reverse transcription-polymerase chain reaction. Bacterial challenges were performed with live NTHi. To examine the role of Th17 cells, bacterial clearance was also evaluated after interleukin (IL)-17 neutralization. P6-specific nasal wash immunoglobulin (Ig) A and serum IgG were increased after immunization with P6 and α-GalCer. Specific IgA-producing cells increased markedly in the nasal passages (NPs) of the immunized mice. In addition to P6-specific Th1 and Th2 cells, IL-17-producing Th17 cells were induced in the NPs and spleen. Bacterial clearance was enhanced by nasal vaccination. Interestingly, impaired NTHi clearance was shown after IL-17 neutralization. These findings suggest that nasal vaccination with P6 and α-GalCer is an effective regimen for the induction of NTHi-specific protective immunity in the upper respiratory tract. In addition to antigen-specific secretory-IgA, specific Th17 cells induced by nasal vaccination contribute to protection against NTHi.

Authors+Show Affiliations

Department of Otolaryngology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hazama-cho, Yufu, Oita 879-5593, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21605159

Citation

Noda, Kenji, et al. "Th17 Cells Contribute to Nontypeable Haemophilus Influenzae-specific Protective Immunity Induced By Nasal Vaccination With P6 Outer Membrane Protein and Α-galactosylceramide." Microbiology and Immunology, vol. 55, no. 8, 2011, pp. 574-81.
Noda K, Kodama S, Umemoto S, et al. Th17 cells contribute to nontypeable Haemophilus influenzae-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and α-galactosylceramide. Microbiol Immunol. 2011;55(8):574-81.
Noda, K., Kodama, S., Umemoto, S., Nomi, N., Hirano, T., & Suzuki, M. (2011). Th17 cells contribute to nontypeable Haemophilus influenzae-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and α-galactosylceramide. Microbiology and Immunology, 55(8), 574-81. https://doi.org/10.1111/j.1348-0421.2011.00352.x
Noda K, et al. Th17 Cells Contribute to Nontypeable Haemophilus Influenzae-specific Protective Immunity Induced By Nasal Vaccination With P6 Outer Membrane Protein and Α-galactosylceramide. Microbiol Immunol. 2011;55(8):574-81. PubMed PMID: 21605159.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Th17 cells contribute to nontypeable Haemophilus influenzae-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and α-galactosylceramide. AU - Noda,Kenji, AU - Kodama,Satoru, AU - Umemoto,Shingo, AU - Nomi,Nozomi, AU - Hirano,Takashi, AU - Suzuki,Masashi, PY - 2011/5/25/entrez PY - 2011/5/25/pubmed PY - 2011/11/16/medline SP - 574 EP - 81 JF - Microbiology and immunology JO - Microbiol Immunol VL - 55 IS - 8 N2 - Nasal vaccination is an effective therapeutic means of preventing upper respiratory infection. Recently, nasal vaccination with P6 outer membrane protein of nontypeable Haemophilus influenzae (NTHi) and alpha-galactosylceramide (α-GalCer) was reported to induce NTHi-specific protective immunity. The present study investigated the role of the Th17 cells induced by nasal vaccination. Mice were immunized with P6 and α-GalCer, and their P6-specific immune responses were examined. Cytokine-producing cells were analyzed by flow cytometry, and expression of cytokines in P6-specific CD4+ T cells was determined by reverse transcription-polymerase chain reaction. Bacterial challenges were performed with live NTHi. To examine the role of Th17 cells, bacterial clearance was also evaluated after interleukin (IL)-17 neutralization. P6-specific nasal wash immunoglobulin (Ig) A and serum IgG were increased after immunization with P6 and α-GalCer. Specific IgA-producing cells increased markedly in the nasal passages (NPs) of the immunized mice. In addition to P6-specific Th1 and Th2 cells, IL-17-producing Th17 cells were induced in the NPs and spleen. Bacterial clearance was enhanced by nasal vaccination. Interestingly, impaired NTHi clearance was shown after IL-17 neutralization. These findings suggest that nasal vaccination with P6 and α-GalCer is an effective regimen for the induction of NTHi-specific protective immunity in the upper respiratory tract. In addition to antigen-specific secretory-IgA, specific Th17 cells induced by nasal vaccination contribute to protection against NTHi. SN - 1348-0421 UR - https://www.unboundmedicine.com/medline/citation/21605159/Th17_cells_contribute_to_nontypeable_Haemophilus_influenzae_specific_protective_immunity_induced_by_nasal_vaccination_with_P6_outer_membrane_protein_and_α_galactosylceramide_ DB - PRIME DP - Unbound Medicine ER -