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Preclinical evaluation of caprylic acid-fractionated IgG antivenom for the treatment of Taipan (Oxyuranus scutellatus) envenoming in Papua New Guinea.
PLoS Negl Trop Dis. 2011 May; 5(5):e1144.PN

Abstract

BACKGROUND

Snake bite is a common medical emergency in Papua New Guinea (PNG). The taipan, Oxyuranus scutellatus, inflicts a large number of bites that, in the absence of antivenom therapy, result in high mortality. Parenteral administration of antivenoms manufactured in Australia is the current treatment of choice for these envenomings. However, the price of these products is high and has increased over the last 25 years; consequently the country can no longer afford all the antivenom it needs. This situation prompted an international collaborative project aimed at generating a new, low-cost antivenom against O. scutellatus for PNG.

METHODOLOGY/PRINCIPAL FINDINGS

A new monospecific equine whole IgG antivenom, obtained by caprylic acid fractionation of plasma, was prepared by immunising horses with the venom of O. scutellatus from PNG. This antivenom was compared with the currently used F(ab')(2) monospecific taipan antivenom manufactured by CSL Limited, Australia. The comparison included physicochemical properties and the preclinical assessment of the neutralisation of lethal neurotoxicity and the myotoxic, coagulant and phospholipase A(2) activities of the venom of O. scutellatus from PNG. The F(ab')(2) antivenom had a higher protein concentration than whole IgG antivenom. Both antivenoms effectively neutralised, and had similar potency, against the lethal neurotoxic effect (both by intraperitoneal and intravenous routes of injection), myotoxicity, and phospholipase A(2) activity of O. scutellatus venom. However, the whole IgG antivenom showed a higher potency than the F(ab')(2) antivenom in the neutralisation of the coagulant activity of O. scutellatus venom from PNG.

CONCLUSIONS/SIGNIFICANCE

The new whole IgG taipan antivenom described in this study compares favourably with the currently used F(ab')(2) antivenom, both in terms of physicochemical characteristics and neutralising potency. Therefore, it should be considered as a promising low-cost candidate for the treatment of envenomings by O. scutellatus in PNG, and is ready to be tested in clinical trials.

Authors+Show Affiliations

Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21610854

Citation

Vargas, Mariángela, et al. "Preclinical Evaluation of Caprylic Acid-fractionated IgG Antivenom for the Treatment of Taipan (Oxyuranus Scutellatus) Envenoming in Papua New Guinea." PLoS Neglected Tropical Diseases, vol. 5, no. 5, 2011, pp. e1144.
Vargas M, Segura A, Herrera M, et al. Preclinical evaluation of caprylic acid-fractionated IgG antivenom for the treatment of Taipan (Oxyuranus scutellatus) envenoming in Papua New Guinea. PLoS Negl Trop Dis. 2011;5(5):e1144.
Vargas, M., Segura, A., Herrera, M., Villalta, M., Estrada, R., Cerdas, M., Paiva, O., Matainaho, T., Jensen, S. D., Winkel, K. D., León, G., Gutiérrez, J. M., & Williams, D. J. (2011). Preclinical evaluation of caprylic acid-fractionated IgG antivenom for the treatment of Taipan (Oxyuranus scutellatus) envenoming in Papua New Guinea. PLoS Neglected Tropical Diseases, 5(5), e1144. https://doi.org/10.1371/journal.pntd.0001144
Vargas M, et al. Preclinical Evaluation of Caprylic Acid-fractionated IgG Antivenom for the Treatment of Taipan (Oxyuranus Scutellatus) Envenoming in Papua New Guinea. PLoS Negl Trop Dis. 2011;5(5):e1144. PubMed PMID: 21610854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preclinical evaluation of caprylic acid-fractionated IgG antivenom for the treatment of Taipan (Oxyuranus scutellatus) envenoming in Papua New Guinea. AU - Vargas,Mariángela, AU - Segura,Alvaro, AU - Herrera,María, AU - Villalta,Mauren, AU - Estrada,Ricardo, AU - Cerdas,Maykel, AU - Paiva,Owen, AU - Matainaho,Teatulohi, AU - Jensen,Simon D, AU - Winkel,Kenneth D, AU - León,Guillermo, AU - Gutiérrez,José María, AU - Williams,David J, Y1 - 2011/05/17/ PY - 2010/10/15/received PY - 2011/02/27/accepted PY - 2011/5/26/entrez PY - 2011/5/26/pubmed PY - 2011/9/9/medline SP - e1144 EP - e1144 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 5 IS - 5 N2 - BACKGROUND: Snake bite is a common medical emergency in Papua New Guinea (PNG). The taipan, Oxyuranus scutellatus, inflicts a large number of bites that, in the absence of antivenom therapy, result in high mortality. Parenteral administration of antivenoms manufactured in Australia is the current treatment of choice for these envenomings. However, the price of these products is high and has increased over the last 25 years; consequently the country can no longer afford all the antivenom it needs. This situation prompted an international collaborative project aimed at generating a new, low-cost antivenom against O. scutellatus for PNG. METHODOLOGY/PRINCIPAL FINDINGS: A new monospecific equine whole IgG antivenom, obtained by caprylic acid fractionation of plasma, was prepared by immunising horses with the venom of O. scutellatus from PNG. This antivenom was compared with the currently used F(ab')(2) monospecific taipan antivenom manufactured by CSL Limited, Australia. The comparison included physicochemical properties and the preclinical assessment of the neutralisation of lethal neurotoxicity and the myotoxic, coagulant and phospholipase A(2) activities of the venom of O. scutellatus from PNG. The F(ab')(2) antivenom had a higher protein concentration than whole IgG antivenom. Both antivenoms effectively neutralised, and had similar potency, against the lethal neurotoxic effect (both by intraperitoneal and intravenous routes of injection), myotoxicity, and phospholipase A(2) activity of O. scutellatus venom. However, the whole IgG antivenom showed a higher potency than the F(ab')(2) antivenom in the neutralisation of the coagulant activity of O. scutellatus venom from PNG. CONCLUSIONS/SIGNIFICANCE: The new whole IgG taipan antivenom described in this study compares favourably with the currently used F(ab')(2) antivenom, both in terms of physicochemical characteristics and neutralising potency. Therefore, it should be considered as a promising low-cost candidate for the treatment of envenomings by O. scutellatus in PNG, and is ready to be tested in clinical trials. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/21610854/Preclinical_evaluation_of_caprylic_acid_fractionated_IgG_antivenom_for_the_treatment_of_Taipan__Oxyuranus_scutellatus__envenoming_in_Papua_New_Guinea_ L2 - http://dx.plos.org/10.1371/journal.pntd.0001144 DB - PRIME DP - Unbound Medicine ER -