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[Expression of nuclear factor kappaB and tumor necrosis factor α in lung tissue of paraquat poisoned rats].

Abstract

OBJECTIVE

To investigate the expression of nuclear factor kappa B (NF-kgr;B) and tumor necrosis factor α (TNF-α) in lung tissue of acute paraquat poisoned rats.

METHODS

68 male Wistar rats were randomly divided into 2 groups: the control group (n = 8), the intoxication group (n = 60). On the 1st, the 3rd, the 7th, the 14th and the 28th day after intoxication, the expression of NF-κB p65 and TNF-α in lung tissue were detected by LSAB immunohistochemistry (IH) staining. Meanwhile, the level of malondialdehyde (MDA) in plasma, and lung homogenate, the content of malondialdehyde (HPY) in lung homogenate were detected.

RESULTS

The levels of MDA in plasma on the 1st, the 3rd, the 7th day and in lung homogenate on the 1st, the 3rd day of the intoxication group [in plasma: (10.15 ± 3.15), (6.97 ± 1.65) and (5.44 ± 0.66) nmol/ml; in lung homogenate: (10.20 ± 2.43), (10.71 ± 171) nmol/ml] were significantly higher than that of the control group [in plasma: (3.84 ± 1.04) nmol/ml, in lung homogenate: (7.66 ± 0.66) nmol/ml]. The content of HPY in lung homogenate on the 14th and the 28th day after intoxication [(19.98 ± 2.86), (26.06 ± 4.06) µg/0.1 g lung homogenate] were higher than that of the control group [(8.80 ± 1.26) µg/0.1 g lung homogenate] significantly. The expression of NF-κB p65 and TNF-α in lung tissue were both significantly increased on the first day and the 3rd day of the intoxication group compared with the control group and weakened obviously after the 7th day.

CONCLUSION

Acute paraquat poisoning can induce increased expression of both NF-κB p65 and TNF-α in lung tissue; the enhanced activity of NF-κB may take part in the process of pulmonary injury in PQ poisoning.

Authors+Show Affiliations

Department of Emergency, Hebei Medical University, Shijiazhuang, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

21619797

Citation

Hu, Jun-li, et al. "[Expression of Nuclear Factor kappaB and Tumor Necrosis Factor Α in Lung Tissue of Paraquat Poisoned Rats]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, vol. 29, no. 1, 2011, pp. 44-8.
Hu JL, Shi HW, Tian YP, et al. [Expression of nuclear factor kappaB and tumor necrosis factor α in lung tissue of paraquat poisoned rats]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2011;29(1):44-8.
Hu, J. L., Shi, H. W., Tian, Y. P., Wang, X., Gao, H. B., & Tong, F. (2011). [Expression of nuclear factor kappaB and tumor necrosis factor α in lung tissue of paraquat poisoned rats]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, 29(1), 44-8.
Hu JL, et al. [Expression of Nuclear Factor kappaB and Tumor Necrosis Factor Α in Lung Tissue of Paraquat Poisoned Rats]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2011;29(1):44-8. PubMed PMID: 21619797.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of nuclear factor kappaB and tumor necrosis factor α in lung tissue of paraquat poisoned rats]. AU - Hu,Jun-li, AU - Shi,Han-wen, AU - Tian,Ying-ping, AU - Wang,Xia, AU - Gao,Heng-bo, AU - Tong,Fei, PY - 2011/5/31/entrez PY - 2011/5/31/pubmed PY - 2012/9/14/medline SP - 44 EP - 8 JF - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi VL - 29 IS - 1 N2 - OBJECTIVE: To investigate the expression of nuclear factor kappa B (NF-kgr;B) and tumor necrosis factor α (TNF-α) in lung tissue of acute paraquat poisoned rats. METHODS: 68 male Wistar rats were randomly divided into 2 groups: the control group (n = 8), the intoxication group (n = 60). On the 1st, the 3rd, the 7th, the 14th and the 28th day after intoxication, the expression of NF-κB p65 and TNF-α in lung tissue were detected by LSAB immunohistochemistry (IH) staining. Meanwhile, the level of malondialdehyde (MDA) in plasma, and lung homogenate, the content of malondialdehyde (HPY) in lung homogenate were detected. RESULTS: The levels of MDA in plasma on the 1st, the 3rd, the 7th day and in lung homogenate on the 1st, the 3rd day of the intoxication group [in plasma: (10.15 ± 3.15), (6.97 ± 1.65) and (5.44 ± 0.66) nmol/ml; in lung homogenate: (10.20 ± 2.43), (10.71 ± 171) nmol/ml] were significantly higher than that of the control group [in plasma: (3.84 ± 1.04) nmol/ml, in lung homogenate: (7.66 ± 0.66) nmol/ml]. The content of HPY in lung homogenate on the 14th and the 28th day after intoxication [(19.98 ± 2.86), (26.06 ± 4.06) µg/0.1 g lung homogenate] were higher than that of the control group [(8.80 ± 1.26) µg/0.1 g lung homogenate] significantly. The expression of NF-κB p65 and TNF-α in lung tissue were both significantly increased on the first day and the 3rd day of the intoxication group compared with the control group and weakened obviously after the 7th day. CONCLUSION: Acute paraquat poisoning can induce increased expression of both NF-κB p65 and TNF-α in lung tissue; the enhanced activity of NF-κB may take part in the process of pulmonary injury in PQ poisoning. SN - 1001-9391 UR - https://www.unboundmedicine.com/medline/citation/21619797/[Expression_of_nuclear_factor_kappaB_and_tumor_necrosis_factor_α_in_lung_tissue_of_paraquat_poisoned_rats]_ DB - PRIME DP - Unbound Medicine ER -
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