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Ischemic insult induced apoptotic changes in PC12 cells: protection by trans resveratrol.
Eur J Pharmacol. 2011 Sep; 666(1-3):5-11.EJ

Abstract

In this study, we determined the protective potential of trans resveratrol against oxygen-glucose deprivation (OGD) induced reactive oxygen species mediated apoptotic damages in PC12 cells. In vitro model of ischemic cerebral stroke was created by keeping cells in an OGD condition for 6h followed by 24h reoxygenation. Cells received biologically safe doses (5, 10, and 25 μM) of trans resveratrol in the following schedules for 24h prior to OGD; during 6h of OGD; for 24h post OGD and whole treatment group which starts from 24h before OGD and lasted to 24h post OGD. Anti-ischemic potential of trans resveratrol was assessed by measuring the regulation of lipid peroxidation, reactive oxygen species production, glutathione content, and expression (mRNA and protein) of apoptotic markers such as Bax, Bcl(2) and Caspase-3. Hypoxia inducible factor-1α (HIF-1α) was also assessed to correlate the changes with ischemic injuries. Significant (P<0.05) restoration in lipid peroxidation, reactive oxygen species, and glutathione content were observed following the treatment of trans resveratrol in cells receiving OGD and re-oxygenation. Changes induced by trans resveratrol could be correlated well with alterations in the expression of Bax, Bcl(2), Caspase-3 and HIF-1α. These results indicate that trans resveratrol administration attenuates free radical formation and mitochondria mediated apoptosis perhaps by regulating the expressions of Bax, Bcl(2,) and Caspase-3 in PC12 cells receiving OGD and re-oxygenation insult.

Authors+Show Affiliations

Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21620820

Citation

Agrawal, Megha, et al. "Ischemic Insult Induced Apoptotic Changes in PC12 Cells: Protection By Trans Resveratrol." European Journal of Pharmacology, vol. 666, no. 1-3, 2011, pp. 5-11.
Agrawal M, Kumar V, Kashyap MP, et al. Ischemic insult induced apoptotic changes in PC12 cells: protection by trans resveratrol. Eur J Pharmacol. 2011;666(1-3):5-11.
Agrawal, M., Kumar, V., Kashyap, M. P., Khanna, V. K., Randhawa, G. S., & Pant, A. B. (2011). Ischemic insult induced apoptotic changes in PC12 cells: protection by trans resveratrol. European Journal of Pharmacology, 666(1-3), 5-11. https://doi.org/10.1016/j.ejphar.2011.05.015
Agrawal M, et al. Ischemic Insult Induced Apoptotic Changes in PC12 Cells: Protection By Trans Resveratrol. Eur J Pharmacol. 2011;666(1-3):5-11. PubMed PMID: 21620820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ischemic insult induced apoptotic changes in PC12 cells: protection by trans resveratrol. AU - Agrawal,Megha, AU - Kumar,Vivek, AU - Kashyap,Mahendra P, AU - Khanna,Vinay K, AU - Randhawa,Gursharn S, AU - Pant,Aditya B, Y1 - 2011/05/23/ PY - 2010/12/23/received PY - 2011/04/25/revised PY - 2011/05/11/accepted PY - 2011/5/31/entrez PY - 2011/5/31/pubmed PY - 2011/10/26/medline SP - 5 EP - 11 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 666 IS - 1-3 N2 - In this study, we determined the protective potential of trans resveratrol against oxygen-glucose deprivation (OGD) induced reactive oxygen species mediated apoptotic damages in PC12 cells. In vitro model of ischemic cerebral stroke was created by keeping cells in an OGD condition for 6h followed by 24h reoxygenation. Cells received biologically safe doses (5, 10, and 25 μM) of trans resveratrol in the following schedules for 24h prior to OGD; during 6h of OGD; for 24h post OGD and whole treatment group which starts from 24h before OGD and lasted to 24h post OGD. Anti-ischemic potential of trans resveratrol was assessed by measuring the regulation of lipid peroxidation, reactive oxygen species production, glutathione content, and expression (mRNA and protein) of apoptotic markers such as Bax, Bcl(2) and Caspase-3. Hypoxia inducible factor-1α (HIF-1α) was also assessed to correlate the changes with ischemic injuries. Significant (P<0.05) restoration in lipid peroxidation, reactive oxygen species, and glutathione content were observed following the treatment of trans resveratrol in cells receiving OGD and re-oxygenation. Changes induced by trans resveratrol could be correlated well with alterations in the expression of Bax, Bcl(2), Caspase-3 and HIF-1α. These results indicate that trans resveratrol administration attenuates free radical formation and mitochondria mediated apoptosis perhaps by regulating the expressions of Bax, Bcl(2,) and Caspase-3 in PC12 cells receiving OGD and re-oxygenation insult. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/21620820/Ischemic_insult_induced_apoptotic_changes_in_PC12_cells:_protection_by_trans_resveratrol_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(11)00545-0 DB - PRIME DP - Unbound Medicine ER -