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A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae.
J Genet Genomics. 2011 May 20; 38(5):209-16.JG

Abstract

Rice blast, caused by Magnaporthe oryzae, is one of the most devastating diseases. Using map-based strategy and in silico approach we isolated a new rice (Oryza sativa L.) blast resistance allele of Pid3, designated Pi25, from a stable blast resistance cultivar Gumei2. Over-expression analysis and complementation test showed that Pi25 conferred blast resistance to M. oryzae isolate js001-20. Sequence analysis showed that Pi25 was an intronless gene of 2,772 nucleotides with single nucleotide substitution in comparison to Pid3 at the nucleotide position 459 and predicatively encoded a typical coiled coil--nucleotide binding site--leucine rich repeat (CC--NBS--LRR) protein of 924 amino acid residuals with 100% identity to Pid3 putative protein. The susceptible allele pi25 in Nipponbare contained a nonsense mutation at the nucleotide position 2,209 resulting in a truncated protein with 736 amino acid residuals. In addition, 14 nucleotide substitutions resulting in 10 amino acid substitutions were identified between Pi25 and pi25 upstream the premature stop codon in the susceptible allele. Although the mechanism of Pi25/Pid3-mediated resistance needs to be further investigated, the isolation of the allele would facilitate the utilization of Pi25/Pid3 in rice blast resistance breeding program via transgenic approach and marker assisted selection.

Authors+Show Affiliations

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21621742

Citation

Chen, Jie, et al. "A Pid3 Allele From Rice Cultivar Gumei2 Confers Resistance to Magnaporthe Oryzae." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 38, no. 5, 2011, pp. 209-16.
Chen J, Shi Y, Liu W, et al. A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae. J Genet Genomics. 2011;38(5):209-16.
Chen, J., Shi, Y., Liu, W., Chai, R., Fu, Y., Zhuang, J., & Wu, J. (2011). A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 38(5), 209-16. https://doi.org/10.1016/j.jgg.2011.03.010
Chen J, et al. A Pid3 Allele From Rice Cultivar Gumei2 Confers Resistance to Magnaporthe Oryzae. J Genet Genomics. 2011 May 20;38(5):209-16. PubMed PMID: 21621742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae. AU - Chen,Jie, AU - Shi,Yongfeng, AU - Liu,Wenzheng, AU - Chai,Rongyao, AU - Fu,Yaping, AU - Zhuang,Jieyun, AU - Wu,Jianli, Y1 - 2011/04/08/ PY - 2011/01/25/received PY - 2011/03/23/revised PY - 2011/03/28/accepted PY - 2011/5/31/entrez PY - 2011/5/31/pubmed PY - 2012/2/24/medline SP - 209 EP - 16 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 38 IS - 5 N2 - Rice blast, caused by Magnaporthe oryzae, is one of the most devastating diseases. Using map-based strategy and in silico approach we isolated a new rice (Oryza sativa L.) blast resistance allele of Pid3, designated Pi25, from a stable blast resistance cultivar Gumei2. Over-expression analysis and complementation test showed that Pi25 conferred blast resistance to M. oryzae isolate js001-20. Sequence analysis showed that Pi25 was an intronless gene of 2,772 nucleotides with single nucleotide substitution in comparison to Pid3 at the nucleotide position 459 and predicatively encoded a typical coiled coil--nucleotide binding site--leucine rich repeat (CC--NBS--LRR) protein of 924 amino acid residuals with 100% identity to Pid3 putative protein. The susceptible allele pi25 in Nipponbare contained a nonsense mutation at the nucleotide position 2,209 resulting in a truncated protein with 736 amino acid residuals. In addition, 14 nucleotide substitutions resulting in 10 amino acid substitutions were identified between Pi25 and pi25 upstream the premature stop codon in the susceptible allele. Although the mechanism of Pi25/Pid3-mediated resistance needs to be further investigated, the isolation of the allele would facilitate the utilization of Pi25/Pid3 in rice blast resistance breeding program via transgenic approach and marker assisted selection. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/21621742/A_Pid3_allele_from_rice_cultivar_Gumei2_confers_resistance_to_Magnaporthe_oryzae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1673-8527(11)00056-7 DB - PRIME DP - Unbound Medicine ER -