Tags

Type your tag names separated by a space and hit enter

Hydrazone-based ligands for micro-solid phase extraction-high performance liquid chromatographic determination of biogenic amines in orange juice.
J Chromatogr A. 2011 Jul 15; 1218(28):4332-9.JC

Abstract

Eight hydrazone-based ligands were synthesized, trapped in a silica sol-gel matrix, and were subsequently used in the micro-solid phase extraction (μ-SPE) of biogenic amines (BAs). The BAs investigated were tryptamine, phenylethylamine, putrescine, histamine, tyramine and spermidine. Prior to the extraction, dansyl chloride was added to the samples which were heated to 70°C for 10 min. The samples were extracted with μ-SPE, after which analytes were desorbed using acetonitrile via ultrasonication. The extracts were analysed by high performance liquid chromatography (HPLC) with ultraviolet detection. Of the eight ligands investigated as sorbents, benzophenone 2,4-dinitrophenylhydrazone was found to be the most promising. The enhanced π-π interaction between the analytes and the ligand facilitated the adsorption process. Under the most suitable extraction conditions, the method demonstrated good linearity with correlation coefficient of more than 0.985 over a concentration range of 1-50 μg L(-1). Satisfactory repeatability with relative standard deviations of 7.43-11.30% (n=3) were obtained. Detection limits ranged from 3.8 to 31.3 ng L(-1). The μ-SPE method exhibited lower recoveries (71.5-87.4%) when compared to the solid phase extraction technique (79.7-95.0%), but enrichment factors of 94-460 were obtained. The proposed μ-SPE-HPLC method was applied to the determination of BAs in orange juice purchased from local supermarkets, with satisfactory results. The orange juices were characterized by the presence of relatively high levels of putrescine (range, 550-2210 μg L(-1)) but tryptamine and phenylethylamine were not detected in any of the tested samples.

Authors+Show Affiliations

Department of Chemistry, King Fahd University of Petroleum and Minerals, KFUPM Box 1059, Dhahran, Saudi Arabia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21621782

Citation

Basheer, Chanbasha, et al. "Hydrazone-based Ligands for Micro-solid Phase Extraction-high Performance Liquid Chromatographic Determination of Biogenic Amines in Orange Juice." Journal of Chromatography. A, vol. 1218, no. 28, 2011, pp. 4332-9.
Basheer C, Wong W, Makahleh A, et al. Hydrazone-based ligands for micro-solid phase extraction-high performance liquid chromatographic determination of biogenic amines in orange juice. J Chromatogr A. 2011;1218(28):4332-9.
Basheer, C., Wong, W., Makahleh, A., Tameem, A. A., Salhin, A., Saad, B., & Lee, H. K. (2011). Hydrazone-based ligands for micro-solid phase extraction-high performance liquid chromatographic determination of biogenic amines in orange juice. Journal of Chromatography. A, 1218(28), 4332-9. https://doi.org/10.1016/j.chroma.2011.04.073
Basheer C, et al. Hydrazone-based Ligands for Micro-solid Phase Extraction-high Performance Liquid Chromatographic Determination of Biogenic Amines in Orange Juice. J Chromatogr A. 2011 Jul 15;1218(28):4332-9. PubMed PMID: 21621782.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrazone-based ligands for micro-solid phase extraction-high performance liquid chromatographic determination of biogenic amines in orange juice. AU - Basheer,Chanbasha, AU - Wong,Weishan, AU - Makahleh,Ahmad, AU - Tameem,Abdassalam Abdelhafiz, AU - Salhin,Abdussalam, AU - Saad,Bahruddin, AU - Lee,Hian Kee, Y1 - 2011/05/06/ PY - 2011/02/18/received PY - 2011/04/15/revised PY - 2011/04/26/accepted PY - 2011/5/31/entrez PY - 2011/5/31/pubmed PY - 2011/9/17/medline SP - 4332 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1218 IS - 28 N2 - Eight hydrazone-based ligands were synthesized, trapped in a silica sol-gel matrix, and were subsequently used in the micro-solid phase extraction (μ-SPE) of biogenic amines (BAs). The BAs investigated were tryptamine, phenylethylamine, putrescine, histamine, tyramine and spermidine. Prior to the extraction, dansyl chloride was added to the samples which were heated to 70°C for 10 min. The samples were extracted with μ-SPE, after which analytes were desorbed using acetonitrile via ultrasonication. The extracts were analysed by high performance liquid chromatography (HPLC) with ultraviolet detection. Of the eight ligands investigated as sorbents, benzophenone 2,4-dinitrophenylhydrazone was found to be the most promising. The enhanced π-π interaction between the analytes and the ligand facilitated the adsorption process. Under the most suitable extraction conditions, the method demonstrated good linearity with correlation coefficient of more than 0.985 over a concentration range of 1-50 μg L(-1). Satisfactory repeatability with relative standard deviations of 7.43-11.30% (n=3) were obtained. Detection limits ranged from 3.8 to 31.3 ng L(-1). The μ-SPE method exhibited lower recoveries (71.5-87.4%) when compared to the solid phase extraction technique (79.7-95.0%), but enrichment factors of 94-460 were obtained. The proposed μ-SPE-HPLC method was applied to the determination of BAs in orange juice purchased from local supermarkets, with satisfactory results. The orange juices were characterized by the presence of relatively high levels of putrescine (range, 550-2210 μg L(-1)) but tryptamine and phenylethylamine were not detected in any of the tested samples. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/21621782/Hydrazone_based_ligands_for_micro_solid_phase_extraction_high_performance_liquid_chromatographic_determination_of_biogenic_amines_in_orange_juice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(11)00583-8 DB - PRIME DP - Unbound Medicine ER -