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Relevance of syndecan-1 in the trophoblastic BeWo cell syncytialization.
Am J Reprod Immunol. 2011 Nov; 66(5):385-93.AJ

Abstract

PROBLEM

To investigate the role of syndecan-1 in the differentiation of the BeWo cells into syncytiotrophoblast.

METHOD OF STUDY

BeWo cells were stimulated with forskolin to form syncytia, and the expression of syndecan-1, desmoplakin I+II, human chorionic gonadotrophin (hCG) and angiogenesis-associated factors was analyzed. Syndecan-1 was silenced by siRNA to evaluate its involvement in the forskolin-mediated syncytia formation.

RESULTS

Treatment of the BeWo cells with forskolin led to a significant increase in the syncytia formation. It was associated with an increase in the expression of syndecan-1 with a concomitant decrease in the expression of desmoplakin I+II. Forskolin treatment of the BeWo cells also led to an increase in the secretion of soluble endoglin, whereas no change was observed in the soluble fms-like tyrosine kinase-1. Silencing of the syndecan-1 expression in BeWo cells led to a significant decrease in cell fusion both in the presence and in the absence of forskolin. It was associated with a significant decrease in hCG level in the conditioned medium.

CONCLUSION

Syndecan-1 is up-regulated in BeWo cells during differentiation and its silencing inhibits syncytialization and thus could be a useful biomarker for syncytiotrophoblast formation.

Authors+Show Affiliations

Reproductive Cell Biology Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21623993

Citation

Prakash, Golla Jaya, et al. "Relevance of Syndecan-1 in the Trophoblastic BeWo Cell Syncytialization." American Journal of Reproductive Immunology (New York, N.Y. : 1989), vol. 66, no. 5, 2011, pp. 385-93.
Prakash GJ, Suman P, Gupta SK. Relevance of syndecan-1 in the trophoblastic BeWo cell syncytialization. Am J Reprod Immunol. 2011;66(5):385-93.
Prakash, G. J., Suman, P., & Gupta, S. K. (2011). Relevance of syndecan-1 in the trophoblastic BeWo cell syncytialization. American Journal of Reproductive Immunology (New York, N.Y. : 1989), 66(5), 385-93. https://doi.org/10.1111/j.1600-0897.2011.01017.x
Prakash GJ, Suman P, Gupta SK. Relevance of Syndecan-1 in the Trophoblastic BeWo Cell Syncytialization. Am J Reprod Immunol. 2011;66(5):385-93. PubMed PMID: 21623993.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relevance of syndecan-1 in the trophoblastic BeWo cell syncytialization. AU - Prakash,Golla Jaya, AU - Suman,Pankaj, AU - Gupta,Satish Kumar, Y1 - 2011/05/30/ PY - 2011/6/1/entrez PY - 2011/6/1/pubmed PY - 2012/1/27/medline SP - 385 EP - 93 JF - American journal of reproductive immunology (New York, N.Y. : 1989) JO - Am J Reprod Immunol VL - 66 IS - 5 N2 - PROBLEM: To investigate the role of syndecan-1 in the differentiation of the BeWo cells into syncytiotrophoblast. METHOD OF STUDY: BeWo cells were stimulated with forskolin to form syncytia, and the expression of syndecan-1, desmoplakin I+II, human chorionic gonadotrophin (hCG) and angiogenesis-associated factors was analyzed. Syndecan-1 was silenced by siRNA to evaluate its involvement in the forskolin-mediated syncytia formation. RESULTS: Treatment of the BeWo cells with forskolin led to a significant increase in the syncytia formation. It was associated with an increase in the expression of syndecan-1 with a concomitant decrease in the expression of desmoplakin I+II. Forskolin treatment of the BeWo cells also led to an increase in the secretion of soluble endoglin, whereas no change was observed in the soluble fms-like tyrosine kinase-1. Silencing of the syndecan-1 expression in BeWo cells led to a significant decrease in cell fusion both in the presence and in the absence of forskolin. It was associated with a significant decrease in hCG level in the conditioned medium. CONCLUSION: Syndecan-1 is up-regulated in BeWo cells during differentiation and its silencing inhibits syncytialization and thus could be a useful biomarker for syncytiotrophoblast formation. SN - 1600-0897 UR - https://www.unboundmedicine.com/medline/citation/21623993/Relevance_of_syndecan_1_in_the_trophoblastic_BeWo_cell_syncytialization_ L2 - https://doi.org/10.1111/j.1600-0897.2011.01017.x DB - PRIME DP - Unbound Medicine ER -