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Inhibition of selenocysteine tRNA[Ser]Sec aminoacylation provides evidence that aminoacylation is required for regulatory methylation of this tRNA.
Biochem Biophys Res Commun. 2011 Jun 17; 409(4):814-9.BB

Abstract

There are two isoforms of selenocysteine (Sec) tRNA([Ser]Sec) that differ by a single methyl group, Um34. The non-Um34 isoform supports the synthesis of a subclass of selenoproteins, designated housekeeping, while the Um34 isoform supports the expression of another subclass, designated stress-related selenoproteins. Herein, we investigated the relationship between tRNA([Ser]Sec) aminoacylation and Um34 synthesis which is the last step in the maturation of this tRNA. Mutation of the discriminator base at position 73 in tRNA([Ser]Sec) dramatically reduced aminoacylation with serine, as did an inhibitor of seryl-tRNA synthetase, SB-217452. Although both the mutation and the inhibitor prevented Um34 synthesis, neither precluded the synthesis of any other of the known base modifications on tRNA([Ser]Sec) following microinjection and incubation of the mutant tRNA([Ser]Sec) transcript, or the wild type transcript along with inhibitor, in Xenopus oocytes. The data demonstrate that Sec tRNA([Ser]Sec) must be aminoacylated for Um34 addition. The fact that selenium is required for Um34 methylation suggests that Sec must be attached to its tRNA for Um34 methylation. This would explain why selenium is essential for the function of Um34 methylase and provides further insights into the hierarchy of selenoprotein expression.

Authors+Show Affiliations

Laboratory of Molecular Genetics and Genomics, School of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21624347

Citation

Kim, Jin Young, et al. "Inhibition of Selenocysteine tRNA[Ser]Sec Aminoacylation Provides Evidence That Aminoacylation Is Required for Regulatory Methylation of This TRNA." Biochemical and Biophysical Research Communications, vol. 409, no. 4, 2011, pp. 814-9.
Kim JY, Carlson BA, Xu XM, et al. Inhibition of selenocysteine tRNA[Ser]Sec aminoacylation provides evidence that aminoacylation is required for regulatory methylation of this tRNA. Biochem Biophys Res Commun. 2011;409(4):814-9.
Kim, J. Y., Carlson, B. A., Xu, X. M., Zeng, Y., Chen, S., Gladyshev, V. N., Lee, B. J., & Hatfield, D. L. (2011). Inhibition of selenocysteine tRNA[Ser]Sec aminoacylation provides evidence that aminoacylation is required for regulatory methylation of this tRNA. Biochemical and Biophysical Research Communications, 409(4), 814-9. https://doi.org/10.1016/j.bbrc.2011.05.096
Kim JY, et al. Inhibition of Selenocysteine tRNA[Ser]Sec Aminoacylation Provides Evidence That Aminoacylation Is Required for Regulatory Methylation of This TRNA. Biochem Biophys Res Commun. 2011 Jun 17;409(4):814-9. PubMed PMID: 21624347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of selenocysteine tRNA[Ser]Sec aminoacylation provides evidence that aminoacylation is required for regulatory methylation of this tRNA. AU - Kim,Jin Young, AU - Carlson,Bradley A, AU - Xu,Xue-Ming, AU - Zeng,Yu, AU - Chen,Shawn, AU - Gladyshev,Vadim N, AU - Lee,Byeong Jae, AU - Hatfield,Dolph L, Y1 - 2011/05/23/ PY - 2011/05/06/received PY - 2011/05/15/accepted PY - 2011/6/1/entrez PY - 2011/6/1/pubmed PY - 2011/9/3/medline SP - 814 EP - 9 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 409 IS - 4 N2 - There are two isoforms of selenocysteine (Sec) tRNA([Ser]Sec) that differ by a single methyl group, Um34. The non-Um34 isoform supports the synthesis of a subclass of selenoproteins, designated housekeeping, while the Um34 isoform supports the expression of another subclass, designated stress-related selenoproteins. Herein, we investigated the relationship between tRNA([Ser]Sec) aminoacylation and Um34 synthesis which is the last step in the maturation of this tRNA. Mutation of the discriminator base at position 73 in tRNA([Ser]Sec) dramatically reduced aminoacylation with serine, as did an inhibitor of seryl-tRNA synthetase, SB-217452. Although both the mutation and the inhibitor prevented Um34 synthesis, neither precluded the synthesis of any other of the known base modifications on tRNA([Ser]Sec) following microinjection and incubation of the mutant tRNA([Ser]Sec) transcript, or the wild type transcript along with inhibitor, in Xenopus oocytes. The data demonstrate that Sec tRNA([Ser]Sec) must be aminoacylated for Um34 addition. The fact that selenium is required for Um34 methylation suggests that Sec must be attached to its tRNA for Um34 methylation. This would explain why selenium is essential for the function of Um34 methylase and provides further insights into the hierarchy of selenoprotein expression. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/21624347/Inhibition_of_selenocysteine_tRNA[Ser]Sec_aminoacylation_provides_evidence_that_aminoacylation_is_required_for_regulatory_methylation_of_this_tRNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(11)00863-1 DB - PRIME DP - Unbound Medicine ER -