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Improvement of H5N1 influenza vaccine viruses: influence of internal gene segments of avian and human origin on production and hemagglutinin content.
Vaccine. 2011 Jul 18; 29(32):5153-62.V

Abstract

The H5N1-clade 1 influenza vaccine strain NIBRG-14 produces exceptionally low amounts of antigen, a problem recently encountered also for initial pandemic H1N1-2009 vaccine seeds. Here, we report on a strategy that may contribute to overcome this obstacle. Influenza vaccine viruses usually consist of two segments coding for the antigenic HA and NA proteins of a wild-type strain and the six residual internal gene segments of the vaccine donor strain A/PR/8/34 (PR8). To enhance the antigen yield from H5N1 vaccine virus we generated by reverse genetics a set of PR8-based reassortant viruses expressing the HA and NA segments of the prototypic strain A/Vietnam/1203/2004 and additional replacements of the internal M or PB1 genes of PR8. The reassortants were compared to the parental PR8 and H5N1 viruses in terms of growth in embryonated chicken eggs and the amount of incorporated antigenic HA protein. Compared to NIBRG-14, three out of six viruses displayed an increased replication in embryonated chicken eggs and higher HA content that was also maintained after ether/detergent extraction of virions. Electron microscopic analysis showed that the reassortment hardly affected particle shape and size. Two selected H5N1 reassortant viruses were investigated concerning their pathogenicity in ferrets and found to behave as low pathogenic as the PR8 donor strain. In conclusion, this study shows that replication and antigen content of PR8-derived H5N1 influenza vaccine viruses can be improved by incorporation of heterologous internal gene segments without compromising their attenuated character.

Authors+Show Affiliations

Division of Influenza/Respiratory Viruses, Robert Koch-Institut, Nordufer 20, D-13353 Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21624413

Citation

Abt, Marion, et al. "Improvement of H5N1 Influenza Vaccine Viruses: Influence of Internal Gene Segments of Avian and Human Origin On Production and Hemagglutinin Content." Vaccine, vol. 29, no. 32, 2011, pp. 5153-62.
Abt M, de Jonge J, Laue M, et al. Improvement of H5N1 influenza vaccine viruses: influence of internal gene segments of avian and human origin on production and hemagglutinin content. Vaccine. 2011;29(32):5153-62.
Abt, M., de Jonge, J., Laue, M., & Wolff, T. (2011). Improvement of H5N1 influenza vaccine viruses: influence of internal gene segments of avian and human origin on production and hemagglutinin content. Vaccine, 29(32), 5153-62. https://doi.org/10.1016/j.vaccine.2011.05.036
Abt M, et al. Improvement of H5N1 Influenza Vaccine Viruses: Influence of Internal Gene Segments of Avian and Human Origin On Production and Hemagglutinin Content. Vaccine. 2011 Jul 18;29(32):5153-62. PubMed PMID: 21624413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improvement of H5N1 influenza vaccine viruses: influence of internal gene segments of avian and human origin on production and hemagglutinin content. AU - Abt,Marion, AU - de Jonge,Jørgen, AU - Laue,Michael, AU - Wolff,Thorsten, Y1 - 2011/05/30/ PY - 2010/09/10/received PY - 2011/03/31/revised PY - 2011/05/13/accepted PY - 2011/6/1/entrez PY - 2011/6/1/pubmed PY - 2012/1/19/medline SP - 5153 EP - 62 JF - Vaccine JO - Vaccine VL - 29 IS - 32 N2 - The H5N1-clade 1 influenza vaccine strain NIBRG-14 produces exceptionally low amounts of antigen, a problem recently encountered also for initial pandemic H1N1-2009 vaccine seeds. Here, we report on a strategy that may contribute to overcome this obstacle. Influenza vaccine viruses usually consist of two segments coding for the antigenic HA and NA proteins of a wild-type strain and the six residual internal gene segments of the vaccine donor strain A/PR/8/34 (PR8). To enhance the antigen yield from H5N1 vaccine virus we generated by reverse genetics a set of PR8-based reassortant viruses expressing the HA and NA segments of the prototypic strain A/Vietnam/1203/2004 and additional replacements of the internal M or PB1 genes of PR8. The reassortants were compared to the parental PR8 and H5N1 viruses in terms of growth in embryonated chicken eggs and the amount of incorporated antigenic HA protein. Compared to NIBRG-14, three out of six viruses displayed an increased replication in embryonated chicken eggs and higher HA content that was also maintained after ether/detergent extraction of virions. Electron microscopic analysis showed that the reassortment hardly affected particle shape and size. Two selected H5N1 reassortant viruses were investigated concerning their pathogenicity in ferrets and found to behave as low pathogenic as the PR8 donor strain. In conclusion, this study shows that replication and antigen content of PR8-derived H5N1 influenza vaccine viruses can be improved by incorporation of heterologous internal gene segments without compromising their attenuated character. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/21624413/Improvement_of_H5N1_influenza_vaccine_viruses:_influence_of_internal_gene_segments_of_avian_and_human_origin_on_production_and_hemagglutinin_content_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(11)00756-0 DB - PRIME DP - Unbound Medicine ER -