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A novel deletion of the MEN1 gene in a large family of multiple endocrine neoplasia type 1 (MEN1) with aggressive phenotype.
Clin Endocrinol (Oxf). 2011 Dec; 75(6):791-800.CE

Abstract

CONTEXT

The MEN1 syndrome is associated with parathyroid, pancreatic and pituitary tumours and is caused by mutations in the MEN1 gene. In general, there is no genotype-phenotype correlation.

OBJECTIVES

To characterize a large family with MEN1 with aggressive tumour behaviour: malignant pancreatic endocrine tumours were present in five affected subjects and were the presenting features in three subjects.

DESIGN

The coding region of MEN1 was sequenced. Gene copy number analysis was performed by multiplex ligation-dependent probe amplification (MLPA) and array comparative genomic hybridization (aCGH). Loss of heterozygosity (LOH) in tumour tissue was studied by microsatellite analysis. Insulin-like growth factor II (IGF-II) and CDKN1C/p57KIP2 expression were investigated by immunohistochemistry.

RESULTS

Mutation screening by conventional PCR sequence analysis of patients' peripheral blood DNA did not reveal any mutation in the MEN1 or CDKN1B gene. Gene copy number analysis by MLPA and aCGH demonstrated a novel monoallelic deletion of 5 kb genomic DNA involving the MEN1 promoter and exons 1 and 2. LOH analysis indicated somatic deletion of maternal chromosome 11, including MEN1 locus (11q13) and 11p15 imprinting control regions (ICR). Methylation analysis of ICR demonstrated ICR1 hypermethylation and ICR2 hypomethylation in the tumour specimens. ICR1 and ICR2 control the expression of IGF-2 and CDKN1C/p57KIP2, respectively. Immunohistochemistry showed that expression of paternally expressed IGF-2 was up-regulated and the maternally expressed CDKN1C/p57KIP2 was lost in the pancreatic endocrine tumours.

CONCLUSIONS

Gene copy number analysis by MLPA should be considered in patients with negative conventional mutation screening. Although large MEN1 deletion causes MEN1, disruption of imprinted CDKN1C/p57KIP2 and IGF-2 gene expression may contribute to tumour progression and aggressive phenotype.

Authors+Show Affiliations

Department of Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21627674

Citation

Raef, Hussein, et al. "A Novel Deletion of the MEN1 Gene in a Large Family of Multiple Endocrine Neoplasia Type 1 (MEN1) With Aggressive Phenotype." Clinical Endocrinology, vol. 75, no. 6, 2011, pp. 791-800.
Raef H, Zou M, Baitei EY, et al. A novel deletion of the MEN1 gene in a large family of multiple endocrine neoplasia type 1 (MEN1) with aggressive phenotype. Clin Endocrinol (Oxf). 2011;75(6):791-800.
Raef, H., Zou, M., Baitei, E. Y., Al-Rijjal, R. A., Kaya, N., Al-Hamed, M., Monies, D., Abu-Dheim, N. N., Al-Hindi, H., Al-Ghamdi, M. H., Meyer, B. F., & Shi, Y. (2011). A novel deletion of the MEN1 gene in a large family of multiple endocrine neoplasia type 1 (MEN1) with aggressive phenotype. Clinical Endocrinology, 75(6), 791-800. https://doi.org/10.1111/j.1365-2265.2011.04134.x
Raef H, et al. A Novel Deletion of the MEN1 Gene in a Large Family of Multiple Endocrine Neoplasia Type 1 (MEN1) With Aggressive Phenotype. Clin Endocrinol (Oxf). 2011;75(6):791-800. PubMed PMID: 21627674.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel deletion of the MEN1 gene in a large family of multiple endocrine neoplasia type 1 (MEN1) with aggressive phenotype. AU - Raef,Hussein, AU - Zou,Minjing, AU - Baitei,Essa Y, AU - Al-Rijjal,Roua A, AU - Kaya,Namik, AU - Al-Hamed,Mohamed, AU - Monies,Dorota, AU - Abu-Dheim,Nada N, AU - Al-Hindi,Hindi, AU - Al-Ghamdi,Mohammed H, AU - Meyer,Brian F, AU - Shi,Yufei, PY - 2011/6/2/entrez PY - 2011/6/2/pubmed PY - 2012/3/6/medline SP - 791 EP - 800 JF - Clinical endocrinology JO - Clin Endocrinol (Oxf) VL - 75 IS - 6 N2 - CONTEXT: The MEN1 syndrome is associated with parathyroid, pancreatic and pituitary tumours and is caused by mutations in the MEN1 gene. In general, there is no genotype-phenotype correlation. OBJECTIVES: To characterize a large family with MEN1 with aggressive tumour behaviour: malignant pancreatic endocrine tumours were present in five affected subjects and were the presenting features in three subjects. DESIGN: The coding region of MEN1 was sequenced. Gene copy number analysis was performed by multiplex ligation-dependent probe amplification (MLPA) and array comparative genomic hybridization (aCGH). Loss of heterozygosity (LOH) in tumour tissue was studied by microsatellite analysis. Insulin-like growth factor II (IGF-II) and CDKN1C/p57KIP2 expression were investigated by immunohistochemistry. RESULTS: Mutation screening by conventional PCR sequence analysis of patients' peripheral blood DNA did not reveal any mutation in the MEN1 or CDKN1B gene. Gene copy number analysis by MLPA and aCGH demonstrated a novel monoallelic deletion of 5 kb genomic DNA involving the MEN1 promoter and exons 1 and 2. LOH analysis indicated somatic deletion of maternal chromosome 11, including MEN1 locus (11q13) and 11p15 imprinting control regions (ICR). Methylation analysis of ICR demonstrated ICR1 hypermethylation and ICR2 hypomethylation in the tumour specimens. ICR1 and ICR2 control the expression of IGF-2 and CDKN1C/p57KIP2, respectively. Immunohistochemistry showed that expression of paternally expressed IGF-2 was up-regulated and the maternally expressed CDKN1C/p57KIP2 was lost in the pancreatic endocrine tumours. CONCLUSIONS: Gene copy number analysis by MLPA should be considered in patients with negative conventional mutation screening. Although large MEN1 deletion causes MEN1, disruption of imprinted CDKN1C/p57KIP2 and IGF-2 gene expression may contribute to tumour progression and aggressive phenotype. SN - 1365-2265 UR - https://www.unboundmedicine.com/medline/citation/21627674/A_novel_deletion_of_the_MEN1_gene_in_a_large_family_of_multiple_endocrine_neoplasia_type_1__MEN1__with_aggressive_phenotype_ L2 - https://doi.org/10.1111/j.1365-2265.2011.04134.x DB - PRIME DP - Unbound Medicine ER -