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Molecular analysis of NDM-1-producing enterobacterial isolates from Geneva, Switzerland.
J Antimicrob Chemother. 2011 Aug; 66(8):1730-3.JA

Abstract

OBJECTIVES

To analyse the mechanisms responsible for decreased susceptibility or resistance to carbapenems in several enterobacterial isolates recovered in 2009-10 in Geneva University Hospitals, Switzerland.

METHODS

PCR and sequencing were used to identify β-lactamases, 16S RNA methylases and plasmid-mediated quinolone resistance genes. The transferable properties of the plasmids were analysed, as well as their plasmid type. The strains were typed by multilocus sequence typing.

RESULTS

Three patients were found to be positive for NDM-1-producing enterobacterial isolates (one with Escherichia coli and Klebsiella pneumoniae, one with K. pneumoniae only and one with Proteus mirabilis), where NDM-1 stands for New Delhi metallo-β-lactamase-1. The bla(NDM-1) carbapenemase gene was detected in all isolates in addition to genes encoding narrow-spectrum β-lactamases (TEM-1, SHV-11, OXA-1, OXA-9 and OXA-10), extended-spectrum β-lactamases (CTX-M-15, CMY-16 and CMY-30), ArmA and quinolone resistance determinants (Qnr). The bla(NDM-1) gene was located on conjugative IncA/C- or IncF-type plasmids. Upstream of the bla(NDM-1) gene, part of ISAba125, previously identified in NDM-1-negative Acinetobacter baumannii, was found. Downstream of the bla(NDM-1) gene, variable sequences were found.

CONCLUSIONS

This work constitutes the first identification of NDM-1 producers in Switzerland. Interestingly, patients from whom these NDM-1-producing isolates were recovered had a link with the Indian subcontinent or the Balkans.

Authors+Show Affiliations

Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, K.-Bicêtre, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21628303

Citation

Poirel, Laurent, et al. "Molecular Analysis of NDM-1-producing Enterobacterial Isolates From Geneva, Switzerland." The Journal of Antimicrobial Chemotherapy, vol. 66, no. 8, 2011, pp. 1730-3.
Poirel L, Schrenzel J, Cherkaoui A, et al. Molecular analysis of NDM-1-producing enterobacterial isolates from Geneva, Switzerland. J Antimicrob Chemother. 2011;66(8):1730-3.
Poirel, L., Schrenzel, J., Cherkaoui, A., Bernabeu, S., Renzi, G., & Nordmann, P. (2011). Molecular analysis of NDM-1-producing enterobacterial isolates from Geneva, Switzerland. The Journal of Antimicrobial Chemotherapy, 66(8), 1730-3. https://doi.org/10.1093/jac/dkr174
Poirel L, et al. Molecular Analysis of NDM-1-producing Enterobacterial Isolates From Geneva, Switzerland. J Antimicrob Chemother. 2011;66(8):1730-3. PubMed PMID: 21628303.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular analysis of NDM-1-producing enterobacterial isolates from Geneva, Switzerland. AU - Poirel,Laurent, AU - Schrenzel,Jacques, AU - Cherkaoui,Abdessalam, AU - Bernabeu,Sandrine, AU - Renzi,Gesuele, AU - Nordmann,Patrice, Y1 - 2011/05/31/ PY - 2011/6/2/entrez PY - 2011/6/2/pubmed PY - 2011/11/1/medline SP - 1730 EP - 3 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 66 IS - 8 N2 - OBJECTIVES: To analyse the mechanisms responsible for decreased susceptibility or resistance to carbapenems in several enterobacterial isolates recovered in 2009-10 in Geneva University Hospitals, Switzerland. METHODS: PCR and sequencing were used to identify β-lactamases, 16S RNA methylases and plasmid-mediated quinolone resistance genes. The transferable properties of the plasmids were analysed, as well as their plasmid type. The strains were typed by multilocus sequence typing. RESULTS: Three patients were found to be positive for NDM-1-producing enterobacterial isolates (one with Escherichia coli and Klebsiella pneumoniae, one with K. pneumoniae only and one with Proteus mirabilis), where NDM-1 stands for New Delhi metallo-β-lactamase-1. The bla(NDM-1) carbapenemase gene was detected in all isolates in addition to genes encoding narrow-spectrum β-lactamases (TEM-1, SHV-11, OXA-1, OXA-9 and OXA-10), extended-spectrum β-lactamases (CTX-M-15, CMY-16 and CMY-30), ArmA and quinolone resistance determinants (Qnr). The bla(NDM-1) gene was located on conjugative IncA/C- or IncF-type plasmids. Upstream of the bla(NDM-1) gene, part of ISAba125, previously identified in NDM-1-negative Acinetobacter baumannii, was found. Downstream of the bla(NDM-1) gene, variable sequences were found. CONCLUSIONS: This work constitutes the first identification of NDM-1 producers in Switzerland. Interestingly, patients from whom these NDM-1-producing isolates were recovered had a link with the Indian subcontinent or the Balkans. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/21628303/Molecular_analysis_of_NDM_1_producing_enterobacterial_isolates_from_Geneva_Switzerland_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkr174 DB - PRIME DP - Unbound Medicine ER -