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Anti-inflammatory and anti-angiogenic effect of long chain n-3 polyunsaturated fatty acids in intestinal microvascular endothelium.
Clin Nutr. 2011 Oct; 30(5):678-87.CN

Abstract

BACKGROUND & AIMS

The role of endothelial cells in inflammatory bowel disease has been recently emphasized. Endothelial activation and expression of adhesion molecules are critical for leukocytes recruitment into the inflammatory wall. Compelling evidence demonstrated anti-inflammatory effects of long chain n-3 PUFA in inflammatory models. We previously showed that long chain n-3 PUFA (EPA and DHA) inhibited inflammatory response in epithelial and dendritic cells. As long chain n-3 PUFA treatment led to a decreased expression of adhesion molecules in endothelial cells from other organs, we have now investigated their effect on intestinal endothelial cells in vitro and in colitic rats.

METHODS

In vitro study: Primary culture of human intestinal microvascular endothelial cells (HIMEC) were pre-treated with DHA and then incubated with IL-1β. In vivo study: Colitis was induced in 2 groups at day0 by intrarectal injection of 2-4-6-trinitrobenzen sulfonic acid (TNBS). Rats received by gavage either fish oil, rich in EPA and DHA (TNBS+n-3) or an isocaloric isolipidic oil formula for 14 days.

RESULTS

DHA led to a decreased VCAM-1, TLR4, cyclooxygenase-2 and VEGFR2 expression and a decreased production of IL-6, IL-8 and GM-CSF and a reduced production of PGE(2) and LTB(4) (p < 0.001) in IL-1β-induced HIMEC. Similarly, dietary intervention with fish oil rich in EPA and DHA significantly decreased colon production of PGE(2) and LTB(4,) endothelial VCAM-1 and VEGFR2 in rats with colitis.

CONCLUSIONS

Data obtained from in vitro and in vivo studies reveal a potential anti-angiogenic role of long chain n-3 PUFA in intestinal endothelial cells. This protective effect of long chain n-3 PUFA may partly explain the observed benefit of dietary intake of long chain n-3 PUFA in IBD development.

Authors+Show Affiliations

Appareil Digestif Environnement Nutrition (ADEN EA 4311), Medicine, University of Rouen, I.F.R. 23, Institute of Biomedical Research, 22 boulevard Gambetta, 76183 Rouen cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21632157

Citation

Ibrahim, Ayman, et al. "Anti-inflammatory and Anti-angiogenic Effect of Long Chain N-3 Polyunsaturated Fatty Acids in Intestinal Microvascular Endothelium." Clinical Nutrition (Edinburgh, Scotland), vol. 30, no. 5, 2011, pp. 678-87.
Ibrahim A, Mbodji K, Hassan A, et al. Anti-inflammatory and anti-angiogenic effect of long chain n-3 polyunsaturated fatty acids in intestinal microvascular endothelium. Clin Nutr. 2011;30(5):678-87.
Ibrahim, A., Mbodji, K., Hassan, A., Aziz, M., Boukhettala, N., Coëffier, M., Savoye, G., Déchelotte, P., & Marion-Letellier, R. (2011). Anti-inflammatory and anti-angiogenic effect of long chain n-3 polyunsaturated fatty acids in intestinal microvascular endothelium. Clinical Nutrition (Edinburgh, Scotland), 30(5), 678-87. https://doi.org/10.1016/j.clnu.2011.05.002
Ibrahim A, et al. Anti-inflammatory and Anti-angiogenic Effect of Long Chain N-3 Polyunsaturated Fatty Acids in Intestinal Microvascular Endothelium. Clin Nutr. 2011;30(5):678-87. PubMed PMID: 21632157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory and anti-angiogenic effect of long chain n-3 polyunsaturated fatty acids in intestinal microvascular endothelium. AU - Ibrahim,Ayman, AU - Mbodji,Khaly, AU - Hassan,Aktham, AU - Aziz,Moutaz, AU - Boukhettala,Nabile, AU - Coëffier,Moïse, AU - Savoye,Guillaume, AU - Déchelotte,Pierre, AU - Marion-Letellier,Rachel, Y1 - 2011/05/31/ PY - 2011/01/03/received PY - 2011/04/22/revised PY - 2011/05/02/accepted PY - 2011/6/3/entrez PY - 2011/6/3/pubmed PY - 2012/2/24/medline SP - 678 EP - 87 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 30 IS - 5 N2 - BACKGROUND & AIMS: The role of endothelial cells in inflammatory bowel disease has been recently emphasized. Endothelial activation and expression of adhesion molecules are critical for leukocytes recruitment into the inflammatory wall. Compelling evidence demonstrated anti-inflammatory effects of long chain n-3 PUFA in inflammatory models. We previously showed that long chain n-3 PUFA (EPA and DHA) inhibited inflammatory response in epithelial and dendritic cells. As long chain n-3 PUFA treatment led to a decreased expression of adhesion molecules in endothelial cells from other organs, we have now investigated their effect on intestinal endothelial cells in vitro and in colitic rats. METHODS: In vitro study: Primary culture of human intestinal microvascular endothelial cells (HIMEC) were pre-treated with DHA and then incubated with IL-1β. In vivo study: Colitis was induced in 2 groups at day0 by intrarectal injection of 2-4-6-trinitrobenzen sulfonic acid (TNBS). Rats received by gavage either fish oil, rich in EPA and DHA (TNBS+n-3) or an isocaloric isolipidic oil formula for 14 days. RESULTS: DHA led to a decreased VCAM-1, TLR4, cyclooxygenase-2 and VEGFR2 expression and a decreased production of IL-6, IL-8 and GM-CSF and a reduced production of PGE(2) and LTB(4) (p < 0.001) in IL-1β-induced HIMEC. Similarly, dietary intervention with fish oil rich in EPA and DHA significantly decreased colon production of PGE(2) and LTB(4,) endothelial VCAM-1 and VEGFR2 in rats with colitis. CONCLUSIONS: Data obtained from in vitro and in vivo studies reveal a potential anti-angiogenic role of long chain n-3 PUFA in intestinal endothelial cells. This protective effect of long chain n-3 PUFA may partly explain the observed benefit of dietary intake of long chain n-3 PUFA in IBD development. SN - 1532-1983 UR - https://www.unboundmedicine.com/medline/citation/21632157/Anti_inflammatory_and_anti_angiogenic_effect_of_long_chain_n_3_polyunsaturated_fatty_acids_in_intestinal_microvascular_endothelium_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0261-5614(11)00075-6 DB - PRIME DP - Unbound Medicine ER -