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Six color flow cytometry detects plasma cells expressing aberrant immunophenotype in bone marrow of healthy donors.
Cytometry B Clin Cytom. 2011 Sep; 80(5):318-23.CB

Abstract

BACKGROUND

Both normal and malignant plasma cell (PC) populations can be identified using modern flow cytometry (FC) technique in multiple myeloma (MM) patients. Expression of CD19 and CD56 markers is heterogenous on bone marrow PCs of healthy individuals. Little is known about immunophenotypically aberrant (CD19-/CD56+) PCs subpopulation of healthy people.

METHODS

Using six color FC, we analyzed PCs in BM samples of 11 healthy donors (HD) and compared their immunophenotypic properties with clonal PC populations from MM patients.

RESULTS

Both immunophenotypically normal (CD19+/CD56-) and aberrant (CD19-/CD56+) PC populations could be detected in 10 of 11 HDs' BM samples and constituted the median of 60.3% (37.3-72.3) and 9.6% (0-35.7) of BM PCs, respectively. CD19, CD56, CD38, CD45, and CD20 marker expression characteristics were of little value discriminating clonal PCs of MM patients from immunophenotypically aberrant PCs of healthy donors.

CONCLUSIONS

Our findings suggest that aberrant immunophenotype is common in BM PCs of healthy people. Improvements in FC methodology to separate normal and malignant PCs remain an open area for future investigations.

Authors+Show Affiliations

Hematology, Oncology, and Transfusion Medicine Center, Vilnius University Hospital Santariskiu Clinics, Santariskiu 2, Vilnius, Lithuania. valdas.peceliunas@santa.ltNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21634008

Citation

Peceliunas, Valdas, et al. "Six Color Flow Cytometry Detects Plasma Cells Expressing Aberrant Immunophenotype in Bone Marrow of Healthy Donors." Cytometry. Part B, Clinical Cytometry, vol. 80, no. 5, 2011, pp. 318-23.
Peceliunas V, Janiulioniene A, Matuzeviciene R, et al. Six color flow cytometry detects plasma cells expressing aberrant immunophenotype in bone marrow of healthy donors. Cytometry B Clin Cytom. 2011;80(5):318-23.
Peceliunas, V., Janiulioniene, A., Matuzeviciene, R., & Griskevicius, L. (2011). Six color flow cytometry detects plasma cells expressing aberrant immunophenotype in bone marrow of healthy donors. Cytometry. Part B, Clinical Cytometry, 80(5), 318-23. https://doi.org/10.1002/cyto.b.20601
Peceliunas V, et al. Six Color Flow Cytometry Detects Plasma Cells Expressing Aberrant Immunophenotype in Bone Marrow of Healthy Donors. Cytometry B Clin Cytom. 2011;80(5):318-23. PubMed PMID: 21634008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Six color flow cytometry detects plasma cells expressing aberrant immunophenotype in bone marrow of healthy donors. AU - Peceliunas,Valdas, AU - Janiulioniene,Ausra, AU - Matuzeviciene,Reda, AU - Griskevicius,Laimonas, Y1 - 2011/06/01/ PY - 2010/08/22/received PY - 2011/03/18/revised PY - 2011/03/30/accepted PY - 2011/6/3/entrez PY - 2011/6/3/pubmed PY - 2012/12/10/medline SP - 318 EP - 23 JF - Cytometry. Part B, Clinical cytometry JO - Cytometry B Clin Cytom VL - 80 IS - 5 N2 - BACKGROUND: Both normal and malignant plasma cell (PC) populations can be identified using modern flow cytometry (FC) technique in multiple myeloma (MM) patients. Expression of CD19 and CD56 markers is heterogenous on bone marrow PCs of healthy individuals. Little is known about immunophenotypically aberrant (CD19-/CD56+) PCs subpopulation of healthy people. METHODS: Using six color FC, we analyzed PCs in BM samples of 11 healthy donors (HD) and compared their immunophenotypic properties with clonal PC populations from MM patients. RESULTS: Both immunophenotypically normal (CD19+/CD56-) and aberrant (CD19-/CD56+) PC populations could be detected in 10 of 11 HDs' BM samples and constituted the median of 60.3% (37.3-72.3) and 9.6% (0-35.7) of BM PCs, respectively. CD19, CD56, CD38, CD45, and CD20 marker expression characteristics were of little value discriminating clonal PCs of MM patients from immunophenotypically aberrant PCs of healthy donors. CONCLUSIONS: Our findings suggest that aberrant immunophenotype is common in BM PCs of healthy people. Improvements in FC methodology to separate normal and malignant PCs remain an open area for future investigations. SN - 1552-4957 UR - https://www.unboundmedicine.com/medline/citation/21634008/Six_color_flow_cytometry_detects_plasma_cells_expressing_aberrant_immunophenotype_in_bone_marrow_of_healthy_donors_ L2 - https://doi.org/10.1002/cyto.b.20601 DB - PRIME DP - Unbound Medicine ER -