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Evaluation of enzyme immunoassay techniques for diagnosis of the most common intestinal protozoa in fecal samples.
Int J Infect Dis. 2011 Aug; 15(8):e541-4.IJ

Abstract

OBJECTIVE

This study was designed to evaluate the antigen capture enzyme immunoassays (EIAs) Triage parasite panel and TechLab Entamoeba histolytica II in detecting Giardia intestinalis, Cryptosporidium sp, and Entamoeba histolytica in fecal samples in comparison to microscopy, and in differentiating Entamoeba histolytica from Entamoeba dispar.

METHODS

The Triage EIA was evaluated using 100 stool specimens that were tested by standard ova and parasite examination, including staining with both trichrome and modified acid-fast stains. Differentiation between E. histolytica and E. dispar was performed using TechLab.

RESULTS

Microscopic examination revealed that 19% of the samples were positive for Giardia, 4% for Cryptosporidium, and 1% for E. histolytica/E. dispar, and other parasites were found in 5%. By Triage, 23% of the samples were infected with Giardia, 5% with Cryptosporidium, and 2% with E. histolytica/E. dispar. Triage showed a sensitivity of 100% and specificity of 91.5%. The TechLab assay was negative for both samples diagnosed as E. histolytica/E. dispar by Triage, which suggested that they were E. dispar. Both tests showed no cross-reactivity with other intestinal protozoa.

CONCLUSION

These results indicate that antigen detection by EIA has the potential to become a valuable tool, capable of making stool diagnostics more effective.

Authors+Show Affiliations

Parasitology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt. drmahagaafar@yahoo.com

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21636305

Citation

Gaafar, Maha R.. "Evaluation of Enzyme Immunoassay Techniques for Diagnosis of the Most Common Intestinal Protozoa in Fecal Samples." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 15, no. 8, 2011, pp. e541-4.
Gaafar MR. Evaluation of enzyme immunoassay techniques for diagnosis of the most common intestinal protozoa in fecal samples. Int J Infect Dis. 2011;15(8):e541-4.
Gaafar, M. R. (2011). Evaluation of enzyme immunoassay techniques for diagnosis of the most common intestinal protozoa in fecal samples. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 15(8), e541-4. https://doi.org/10.1016/j.ijid.2011.04.004
Gaafar MR. Evaluation of Enzyme Immunoassay Techniques for Diagnosis of the Most Common Intestinal Protozoa in Fecal Samples. Int J Infect Dis. 2011;15(8):e541-4. PubMed PMID: 21636305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of enzyme immunoassay techniques for diagnosis of the most common intestinal protozoa in fecal samples. A1 - Gaafar,Maha R, Y1 - 2011/06/01/ PY - 2010/11/27/received PY - 2011/02/18/revised PY - 2011/04/17/accepted PY - 2011/6/4/entrez PY - 2011/6/4/pubmed PY - 2012/9/21/medline SP - e541 EP - 4 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int J Infect Dis VL - 15 IS - 8 N2 - OBJECTIVE: This study was designed to evaluate the antigen capture enzyme immunoassays (EIAs) Triage parasite panel and TechLab Entamoeba histolytica II in detecting Giardia intestinalis, Cryptosporidium sp, and Entamoeba histolytica in fecal samples in comparison to microscopy, and in differentiating Entamoeba histolytica from Entamoeba dispar. METHODS: The Triage EIA was evaluated using 100 stool specimens that were tested by standard ova and parasite examination, including staining with both trichrome and modified acid-fast stains. Differentiation between E. histolytica and E. dispar was performed using TechLab. RESULTS: Microscopic examination revealed that 19% of the samples were positive for Giardia, 4% for Cryptosporidium, and 1% for E. histolytica/E. dispar, and other parasites were found in 5%. By Triage, 23% of the samples were infected with Giardia, 5% with Cryptosporidium, and 2% with E. histolytica/E. dispar. Triage showed a sensitivity of 100% and specificity of 91.5%. The TechLab assay was negative for both samples diagnosed as E. histolytica/E. dispar by Triage, which suggested that they were E. dispar. Both tests showed no cross-reactivity with other intestinal protozoa. CONCLUSION: These results indicate that antigen detection by EIA has the potential to become a valuable tool, capable of making stool diagnostics more effective. SN - 1878-3511 UR - https://www.unboundmedicine.com/medline/citation/21636305/Evaluation_of_enzyme_immunoassay_techniques_for_diagnosis_of_the_most_common_intestinal_protozoa_in_fecal_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1201-9712(11)00093-2 DB - PRIME DP - Unbound Medicine ER -