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Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus.
Antimicrob Agents Chemother. 2011 Aug; 55(8):3765-73.AA

Abstract

Methicillin resistance in staphylococci is mediated by penicillin binding protein 2a (PBP 2a), encoded by mecA on mobile staphylococcal cassette chromosome mec (SCCmec) elements. In this study, two clonal complex 130 (CC130) methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening. The isolates were identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay. Whole-genome sequencing of one isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. The open reading frames (ORFs) identified within SCCmec XI of M10/0061 exhibited 21 to 93% amino acid identity to ORFs in GenBank. A third DR was identified ca. 3 kb downstream of SCCmec XI, indicating the presence of a possible SCC remnant. SCCmec XI was also identified in the second CC130 MRSA isolate by PCR and sequencing. The CC130 MRSA isolates may be of animal origin as previously reported CC130 S. aureus strains were predominantly from bovine sources. The highly divergent nature of SCCmec XI relative to other SCCmec elements indicates that it may have originated in another taxon.

Authors+Show Affiliations

Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21636525

Citation

Shore, Anna C., et al. "Detection of Staphylococcal Cassette Chromosome Mec Type XI Carrying Highly Divergent mecA, mecI, mecR1, blaZ, and Ccr Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-resistant Staphylococcus Aureus." Antimicrobial Agents and Chemotherapy, vol. 55, no. 8, 2011, pp. 3765-73.
Shore AC, Deasy EC, Slickers P, et al. Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2011;55(8):3765-73.
Shore, A. C., Deasy, E. C., Slickers, P., Brennan, G., O'Connell, B., Monecke, S., Ehricht, R., & Coleman, D. C. (2011). Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 55(8), 3765-73. https://doi.org/10.1128/AAC.00187-11
Shore AC, et al. Detection of Staphylococcal Cassette Chromosome Mec Type XI Carrying Highly Divergent mecA, mecI, mecR1, blaZ, and Ccr Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-resistant Staphylococcus Aureus. Antimicrob Agents Chemother. 2011;55(8):3765-73. PubMed PMID: 21636525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. AU - Shore,Anna C, AU - Deasy,Emily C, AU - Slickers,Peter, AU - Brennan,Grainne, AU - O'Connell,Brian, AU - Monecke,Stefan, AU - Ehricht,Ralf, AU - Coleman,David C, Y1 - 2011/06/02/ PY - 2011/6/4/entrez PY - 2011/6/4/pubmed PY - 2012/2/4/medline SP - 3765 EP - 73 JF - Antimicrobial agents and chemotherapy JO - Antimicrob Agents Chemother VL - 55 IS - 8 N2 - Methicillin resistance in staphylococci is mediated by penicillin binding protein 2a (PBP 2a), encoded by mecA on mobile staphylococcal cassette chromosome mec (SCCmec) elements. In this study, two clonal complex 130 (CC130) methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening. The isolates were identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay. Whole-genome sequencing of one isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. The open reading frames (ORFs) identified within SCCmec XI of M10/0061 exhibited 21 to 93% amino acid identity to ORFs in GenBank. A third DR was identified ca. 3 kb downstream of SCCmec XI, indicating the presence of a possible SCC remnant. SCCmec XI was also identified in the second CC130 MRSA isolate by PCR and sequencing. The CC130 MRSA isolates may be of animal origin as previously reported CC130 S. aureus strains were predominantly from bovine sources. The highly divergent nature of SCCmec XI relative to other SCCmec elements indicates that it may have originated in another taxon. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/21636525/Detection_of_staphylococcal_cassette_chromosome_mec_type_XI_carrying_highly_divergent_mecA_mecI_mecR1_blaZ_and_ccr_genes_in_human_clinical_isolates_of_clonal_complex_130_methicillin_resistant_Staphylococcus_aureus_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=21636525 DB - PRIME DP - Unbound Medicine ER -